FastQCFastQC Report
Thu 26 May 2016
SRR1545488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545488_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2443758
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187970.7691841827218571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG207.034848E-445.01
CGTTTTT127800.042.7288741
CGGTCTA3200.041.48437531
TATAGCG1950.040.3846171
GCGATAT2900.040.3448269
GTACGTC451.9297659E-840.044
CGCATCG3150.038.5714321
ATAGGGC14900.038.3557054
TAACGCC3600.038.12512
CGACGGT3550.038.02816828
GACCGAT15750.038.09
TAGGGCG14750.037.9830475
GCGCGAC11050.037.8733029
CGTAAGG4350.037.758622
ACGGGAC6200.037.7419365
GGCGATA11300.037.2345168
AGGGCGA33050.037.1028756
TAAGGGA26150.036.912054
GGGCGAT56550.036.684357
ACGGGTA5050.036.5346575
Produced by FastQC (version 0.11.5)