##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545488_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2443758
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.036703306955925	31.0	31.0	33.0	30.0	34.0
2	31.325420520362492	31.0	31.0	34.0	30.0	34.0
3	31.41626503115284	31.0	31.0	34.0	30.0	34.0
4	35.2168160677121	37.0	35.0	37.0	33.0	37.0
5	35.08358970078052	37.0	35.0	37.0	32.0	37.0
6	35.13693008882222	37.0	35.0	37.0	32.0	37.0
7	35.73770111443113	37.0	35.0	37.0	35.0	37.0
8	35.738880854814596	37.0	35.0	37.0	35.0	37.0
9	37.427638906962144	39.0	37.0	39.0	35.0	39.0
10	36.58003533901475	39.0	35.0	39.0	32.0	39.0
11	36.303530464145794	38.0	35.0	39.0	32.0	39.0
12	35.746115204533346	37.0	35.0	39.0	30.0	39.0
13	35.51018104083956	37.0	35.0	39.0	30.0	39.0
14	36.46436799388483	38.0	35.0	40.0	30.0	41.0
15	36.65191070474245	38.0	35.0	40.0	31.0	41.0
16	36.71613719525419	38.0	35.0	40.0	31.0	41.0
17	36.636279860771815	38.0	35.0	40.0	31.0	41.0
18	36.52144238504795	38.0	35.0	40.0	31.0	41.0
19	36.449433209016604	38.0	35.0	40.0	30.0	41.0
20	36.332958091594996	38.0	35.0	40.0	30.0	41.0
21	36.20702786446121	38.0	34.0	40.0	30.0	41.0
22	36.12674741115937	38.0	34.0	40.0	30.0	41.0
23	36.0527511316587	38.0	34.0	40.0	30.0	41.0
24	35.956458045354736	38.0	34.0	40.0	30.0	41.0
25	35.83345077540411	37.0	34.0	40.0	30.0	41.0
26	35.67929844117134	37.0	34.0	40.0	29.0	41.0
27	35.59284880090418	37.0	34.0	40.0	29.0	41.0
28	35.57537612153086	37.0	34.0	40.0	29.0	41.0
29	35.53664438131763	37.0	34.0	40.0	29.0	41.0
30	35.42823389222664	37.0	34.0	40.0	29.0	41.0
31	35.27071624931765	37.0	34.0	40.0	27.0	41.0
32	35.12807405643276	37.0	34.0	40.0	27.0	41.0
33	34.889840156022	37.0	33.0	40.0	25.0	41.0
34	34.69291230964768	37.0	33.0	40.0	25.0	41.0
35	34.500116214453314	37.0	33.0	40.0	24.0	41.0
36	34.358386959756245	37.0	33.0	40.0	23.0	41.0
37	34.299601679053325	37.0	33.0	40.0	23.0	41.0
38	34.24684604613059	36.0	33.0	40.0	23.0	41.0
39	34.172028081340294	36.0	33.0	40.0	23.0	41.0
40	34.05418007838747	36.0	33.0	40.0	23.0	41.0
41	33.95704689253191	36.0	33.0	40.0	23.0	41.0
42	33.9066679270206	36.0	33.0	40.0	23.0	41.0
43	33.82910910163773	36.0	33.0	40.0	23.0	41.0
44	33.7071170713303	36.0	33.0	40.0	23.0	41.0
45	33.62855978374291	35.0	32.0	40.0	23.0	41.0
46	33.54931380275789	35.0	32.0	40.0	23.0	41.0
47	33.451795963430094	35.0	32.0	39.0	22.0	41.0
48	33.37407018207204	35.0	32.0	39.0	22.0	41.0
49	33.33161262285382	35.0	32.0	39.0	22.0	41.0
50	33.274600430975575	35.0	32.0	39.0	22.0	41.0
51	33.111329354215925	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	11.0
14	34.0
15	114.0
16	308.0
17	830.0
18	2035.0
19	3649.0
20	6081.0
21	9439.0
22	14107.0
23	19695.0
24	27392.0
25	37627.0
26	46966.0
27	53949.0
28	59842.0
29	66917.0
30	79503.0
31	94434.0
32	114308.0
33	142445.0
34	213083.0
35	274444.0
36	197455.0
37	236604.0
38	316374.0
39	425924.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56995987327714	23.44761633516903	27.520155432739244	16.46226835881458
2	32.67258869331579	25.115580184289932	29.23616004530727	12.97567107708701
3	28.24133977259614	25.20053131283867	32.81302813126341	13.745100783301783
4	24.816941775740478	28.2096263214279	32.38385306564726	14.58957883718437
5	22.182147332100804	31.786903613205563	31.999649719816777	14.031299334876856
6	20.1662357729366	40.770239933741394	28.608274632758235	10.455249660563771
7	86.76067761210398	3.7492664985649156	7.896649340892184	1.5934065484389206
8	87.87101668823182	2.8842463124417392	7.5932232242308775	1.65151377509557
9	83.03048010482217	4.465131162741973	9.462843702199645	3.041545030236218
10	41.655884093269464	26.12300399630405	17.38547761275871	14.835634297667772
11	36.725608673199226	24.63656384961195	23.41258831684643	15.22523916034239
12	34.067407656568285	21.87139643123419	26.51571882322227	17.545477088975257
13	23.941159476511174	29.998796934884712	26.944975730002724	19.115067858601385
14	19.71238559628245	32.5334996345792	28.98093837442169	18.77317639471666
15	19.518544798625722	23.390695805394806	39.16979504517223	17.92096435080724
16	21.53863025716949	20.50636765178876	38.451393304901714	19.503608786140035
17	21.42523932402472	20.85153276224569	30.064106184000217	27.659121729729375
18	23.436404095659228	22.621798066748017	32.704383985648334	21.237413851944424
19	28.009033627716	24.927222744641657	26.938428436858313	20.12531519078403
20	30.16706236869608	24.461341916834645	25.919219497184255	19.452376217285018
21	25.195088875412374	26.487933747940673	28.78938912936551	19.52758824728144
22	24.340298834827344	23.982243740992356	27.69263568651233	23.984821737667968
23	22.340428143866948	27.39522489542745	27.228923649559405	23.035423311146193
24	22.405000822503702	24.185700875454934	34.13918235766389	19.270115944377473
25	22.25768672675445	24.704451095403062	31.98725078342455	21.050611394417942
26	20.646561566243467	29.242911941362443	28.097872211569232	22.01265428082486
27	20.569999157036005	28.422126904546197	28.99648819563967	22.01138574277813
28	19.459823763236788	27.328565267100917	33.11285323669529	20.098757732967012
29	20.717517855696023	25.100644171804248	32.90976438747209	21.272073585027652
30	22.495271626732272	26.729733467880205	30.39007954142759	20.384915363959934
31	26.64952912686117	25.516233604145743	27.419368038897467	20.414869230095615
32	28.708816503107098	25.069544529368294	27.06339989475226	19.158239072772346
33	26.6690891651301	25.59594689817895	26.71316063210842	21.02180330458253
34	22.741654451872893	26.92746172084143	28.39213211782836	21.938751709457318
35	22.451732127321936	25.89335768926383	30.345803471538506	21.309106711875724
36	27.377383521608934	26.786408474161515	25.716785377275492	20.11942262695406
37	22.90144932517868	30.35034565615744	27.197210198391165	19.55099482027271
38	23.54222472110577	29.82324763745019	25.321574394845968	21.312953246598067
39	22.716815658506285	29.581284235182043	25.703486188075907	21.998413918235766
40	25.138372948548916	25.476254195382687	27.13873468649514	22.246638169573256
41	20.506244890042304	25.606586249538623	28.11583634713421	25.771332513284868
42	22.68878505973177	26.6826338778226	25.31036215533617	25.318218907109458
43	22.18857186349876	26.088303342638675	27.11414141662145	24.608983377241117
44	21.99685893611397	27.70744893725156	28.2693294507885	22.026362675845974
45	21.115920643533443	30.461526877865975	25.605194949745435	22.817357528855148
46	22.859628490218753	29.17473825149626	26.544526913057677	21.421106345227308
47	22.319886011626355	27.489628678453432	27.253557840015258	22.93692746990496
48	23.054492302429292	25.45321590763079	28.940590680419255	22.551701109520664
49	22.524939048792884	25.502647970871095	29.52456830831858	22.447844672017442
50	21.477535828015704	29.224170314736565	27.426815584849233	21.871478272398495
51	20.45026553365759	29.881804990510517	27.024607182871623	22.64332229296027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2586.0
1	3147.0
2	3708.0
3	17220.5
4	30733.0
5	21406.5
6	12080.0
7	12502.5
8	12925.0
9	13467.5
10	14010.0
11	14374.0
12	14738.0
13	14671.5
14	14605.0
15	14059.0
16	13513.0
17	13051.0
18	12589.0
19	12521.5
20	12454.0
21	12302.5
22	12151.0
23	12067.5
24	11984.0
25	12644.0
26	15405.0
27	17506.0
28	20065.0
29	22624.0
30	27720.0
31	32816.0
32	40413.0
33	48010.0
34	53164.0
35	58318.0
36	65058.5
37	71799.0
38	79243.0
39	86687.0
40	109829.5
41	132972.0
42	152638.0
43	172304.0
44	177279.5
45	182255.0
46	182146.5
47	182038.0
48	181676.0
49	181314.0
50	175041.0
51	168768.0
52	162681.0
53	156594.0
54	145335.0
55	134076.0
56	131584.5
57	129093.0
58	124356.5
59	119620.0
60	113616.5
61	107613.0
62	92548.0
63	77483.0
64	67190.0
65	56897.0
66	48197.5
67	39498.0
68	34216.0
69	28934.0
70	23991.0
71	19048.0
72	17187.0
73	15326.0
74	12769.0
75	7608.0
76	5004.0
77	3980.0
78	2956.0
79	2254.0
80	1552.0
81	1092.0
82	632.0
83	427.0
84	222.0
85	172.0
86	122.0
87	82.5
88	43.0
89	34.0
90	25.0
91	18.0
92	11.0
93	6.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2443758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.863455774269774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5889514580615	14.824592101753773
2	5.862442412813859	2.2117184636663527
3	2.4731301821377585	1.3995534544430208
4	1.489432804040714	1.123833993110745
5	1.0200563255744493	0.9620893692368876
6	0.7596512881935205	0.859778908722332
7	0.6081917333613988	0.8030818505177456
8	0.4585887563654458	0.6920454979421571
9	0.4124613625586546	0.7002402004098209
>10	5.344836983244095	24.55730526109798
>50	2.1075642385215057	28.624197055429708
>100	0.8712024121580453	21.86064885887556
>500	0.002617532226821299	0.33715376137186603
>1k	6.543830567053247E-4	0.27033197610607274
>5k	0.0	0.0
>10k+	2.181276855684416E-4	0.7734292473159589
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18797	0.7691841827218571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016572835771790823	0.0
2	0.0	0.0	0.0	0.05818906782095445	0.0
3	0.0	0.0	0.0	0.10136028199191573	0.0
4	0.0	0.0	0.0	0.15234732735401788	0.0
5	0.0	0.0	0.0	0.2394263261746867	0.0
6	0.0	0.0	0.0	0.453768335489848	0.0
7	0.0	0.0	0.0	0.5690825359957901	0.0
8	0.0	0.0	0.0	0.9058998476935932	0.0
9	0.0	0.0	0.0	1.087464470704546	0.0
10	0.0	0.0	0.0	1.3284457790010304	0.0
11	0.0	0.0	0.0	1.452762507580538	0.0
12	0.0	0.0	0.0	1.5522404427934353	0.0
13	4.092058215256993E-5	0.0	0.0	1.6079742756852355	0.0
14	4.092058215256993E-5	0.0	0.0	1.6384191888067476	0.0
15	4.092058215256993E-5	0.0	0.0	1.6611710324835764	0.0
16	4.092058215256993E-5	0.0	0.0	1.7071248462409125	0.0
17	4.092058215256993E-5	0.0	0.0	1.75246485126596	0.0
18	4.092058215256993E-5	0.0	0.0	1.8266129461264167	0.0
19	4.092058215256993E-5	0.0	0.0	1.8617228056133217	0.0
20	4.092058215256993E-5	0.0	0.0	1.904894019784283	0.0
21	4.092058215256993E-5	0.0	0.0	1.9526074185741795	0.0
22	4.092058215256993E-5	0.0	0.0	2.004331034415028	0.0
23	4.092058215256993E-5	0.0	0.0	2.059901184978218	0.0
24	4.092058215256993E-5	0.0	0.0	2.107082616200131	0.0
25	4.092058215256993E-5	0.0	0.0	2.152545382971636	0.0
26	8.184116430513987E-5	0.0	0.0	2.193711488617122	0.0
27	8.184116430513987E-5	0.0	0.0	2.241915934392849	0.0
28	1.227617464577098E-4	0.0	0.0	2.2868058130142184	0.0
29	1.227617464577098E-4	0.0	0.0	2.338979555258745	0.0
30	2.0460291076284968E-4	0.0	0.0	2.423194113328734	0.0
31	2.0460291076284968E-4	0.0	0.0	2.4818742281355193	0.0
32	2.0460291076284968E-4	0.0	0.0	2.539817772463558	0.0
33	2.0460291076284968E-4	0.0	0.0	2.6005029957958192	0.0
34	2.0460291076284968E-4	8.184116430513987E-5	0.0	2.6664260536436095	0.0
35	2.0460291076284968E-4	8.184116430513987E-5	0.0	2.737382343096166	0.0
36	2.0460291076284968E-4	1.227617464577098E-4	0.0	2.802814353958125	0.0
37	2.0460291076284968E-4	1.227617464577098E-4	0.0	2.8716427731387477	0.0
38	2.0460291076284968E-4	1.227617464577098E-4	0.0	2.955448125387211	0.0
39	2.0460291076284968E-4	1.227617464577098E-4	0.0	3.0769822543803436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCG	20	7.034848E-4	45.0	1
CGTTTTT	12780	0.0	42.728874	1
CGGTCTA	320	0.0	41.484375	31
TATAGCG	195	0.0	40.384617	1
GCGATAT	290	0.0	40.344826	9
GTACGTC	45	1.9297659E-8	40.0	44
CGCATCG	315	0.0	38.57143	21
ATAGGGC	1490	0.0	38.355705	4
TAACGCC	360	0.0	38.125	12
CGACGGT	355	0.0	38.028168	28
GACCGAT	1575	0.0	38.0	9
TAGGGCG	1475	0.0	37.983047	5
GCGCGAC	1105	0.0	37.873302	9
CGTAAGG	435	0.0	37.75862	2
ACGGGAC	620	0.0	37.741936	5
GGCGATA	1130	0.0	37.234516	8
AGGGCGA	3305	0.0	37.102875	6
TAAGGGA	2615	0.0	36.91205	4
GGGCGAT	5655	0.0	36.68435	7
ACGGGTA	505	0.0	36.534657	5
>>END_MODULE