FastQCFastQC Report
Thu 26 May 2016
SRR1545487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545487_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3863144
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210220.5441681697601746No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC98110.2539641286993185No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAC207.035356E-445.00000441
TACGTCG207.035356E-445.00000440
TAGTCCG351.2129203E-745.01
CCGCGTA302.1668384E-644.99999642
CGGTCTA2900.044.2241431
CGTTAGG6550.043.2824442
CGTTTTT190250.042.9540061
TACGGGA12200.040.9426234
CGCATCG2000.040.521
CCGGATA501.0822987E-940.520
CGACGGT3250.040.15384728
AGGGCGA34350.039.8908276
TAATGCG850.039.7058831
CTATACG1650.039.5454521
CGTAAGG4950.039.5454522
TCACGAC3350.038.95522325
ATGGTCG2100.038.5714344
ATACCGG2450.038.571432
CGTTGAT5250.038.57142625
CTACGGG6750.038.3333323
Produced by FastQC (version 0.11.5)