FastQCFastQC Report
Thu 26 May 2016
SRR1545486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545486_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4354041
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214990.49377118864980835No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80530.18495462031708015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG406.8230293E-945.036
CACTACG253.892605E-545.01
ACGTTAG2600.042.403851
CGTAAGG6550.041.5648842
CGTTTTT174150.041.072351
ACGTAAG2850.041.052631
CGTTGAT7000.040.8214325
ACGGGAT13800.039.945655
ACGGGTA3600.039.3750045
TTACGAG1150.039.1304361
CATACGA10650.039.08450718
GCGATCG1800.038.7500049
ACATACG10800.038.7517
GGCGATA18500.038.5540548
GACCGAT25550.038.1311159
AACGGGA11400.038.0921064
TCGAATC659.094947E-1238.07692744
CACGACG3500.037.92857426
CTAGTCG1900.037.8947371
TCGTTGA7900.037.87974524
Produced by FastQC (version 0.11.5)