FastQCFastQC Report
Thu 26 May 2016
SRR1545485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545485_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences395629
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.4152880602786954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11770.29750094153866374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.12157854960076232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG207.0276705E-445.0000041
CACGGCA207.0276705E-445.00000435
CGACGGT406.7957444E-945.00000428
GGCGTAG207.0276705E-445.0000041
TCGATAG302.161878E-645.0000041
GTTGTAC207.0276705E-445.00000444
CTACGAA302.161878E-645.00000411
TACGAAT302.161878E-645.00000412
GGAACTA302.161878E-645.0000048
TATTACG207.0276705E-445.0000041
GTGTACG207.0276705E-445.0000041
CGAATAT302.161878E-645.00000414
AATTCGC551.8189894E-1245.00000413
TCGTAAG207.0276705E-445.0000041
TGCTACG207.0276705E-445.0000041
ACCCGCT1200.045.00000434
TTGCACG207.0276705E-445.0000041
GCTAATT207.0276705E-445.00000420
CAATACG207.0276705E-445.0000041
ATCGCGG406.7957444E-945.0000042