##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545485_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	395629
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.045249463512533	31.0	31.0	33.0	30.0	34.0
2	31.310432248394328	31.0	31.0	34.0	28.0	34.0
3	31.425666470354802	31.0	31.0	34.0	30.0	34.0
4	35.19107800489853	37.0	35.0	37.0	32.0	37.0
5	35.050565049579276	37.0	35.0	37.0	32.0	37.0
6	35.095326682321065	37.0	35.0	37.0	32.0	37.0
7	35.710180497385174	37.0	35.0	37.0	35.0	37.0
8	35.705501871703035	37.0	35.0	37.0	35.0	37.0
9	37.36343897944792	39.0	37.0	39.0	35.0	39.0
10	36.632109880721586	39.0	35.0	39.0	32.0	39.0
11	36.23462637976488	38.0	35.0	39.0	31.0	39.0
12	35.05255934221202	35.0	34.0	39.0	30.0	39.0
13	34.454716413609724	35.0	33.0	39.0	26.0	39.0
14	35.32240305943194	37.0	33.0	40.0	26.0	41.0
15	35.80490055076852	37.0	34.0	40.0	30.0	41.0
16	36.03504040401487	37.0	34.0	40.0	31.0	41.0
17	35.98488735658913	36.0	34.0	40.0	31.0	41.0
18	35.927967868887265	36.0	34.0	40.0	30.0	41.0
19	35.825960685389596	36.0	34.0	40.0	30.0	41.0
20	35.669008085858216	36.0	34.0	40.0	30.0	41.0
21	35.49732956886376	35.0	34.0	40.0	30.0	41.0
22	35.35661187627803	35.0	34.0	40.0	29.0	41.0
23	35.347360784977845	35.0	34.0	40.0	29.0	41.0
24	35.254827123390854	35.0	34.0	40.0	29.0	41.0
25	35.15408375018009	35.0	34.0	40.0	29.0	41.0
26	34.935017402667654	35.0	34.0	40.0	29.0	41.0
27	34.859598259985994	35.0	34.0	40.0	28.0	41.0
28	34.880451129720015	35.0	34.0	40.0	28.0	41.0
29	34.91461950463692	36.0	34.0	40.0	29.0	41.0
30	34.854831167583775	36.0	34.0	40.0	28.0	41.0
31	34.65032391457653	35.0	33.0	40.0	27.0	41.0
32	34.455646577980886	35.0	33.0	40.0	26.0	41.0
33	34.25947036238496	35.0	33.0	40.0	25.0	41.0
34	34.13965356432415	35.0	33.0	40.0	25.0	41.0
35	33.95843075204295	35.0	33.0	39.0	24.0	41.0
36	33.71006169921821	35.0	33.0	39.0	23.0	41.0
37	33.620043020102166	35.0	33.0	39.0	23.0	41.0
38	33.61298843108063	35.0	33.0	39.0	23.0	41.0
39	33.57523589019005	35.0	33.0	39.0	23.0	41.0
40	33.437806631970865	35.0	32.0	39.0	23.0	41.0
41	33.41167609047871	35.0	32.0	39.0	23.0	41.0
42	33.365860439957636	35.0	32.0	39.0	23.0	41.0
43	33.234371090086924	35.0	32.0	39.0	23.0	41.0
44	33.1325812819586	35.0	32.0	39.0	23.0	41.0
45	33.03346822401796	35.0	32.0	38.0	23.0	41.0
46	32.9828526220272	35.0	32.0	38.0	23.0	40.0
47	32.92431292953752	35.0	32.0	38.0	23.0	40.0
48	32.90078078199525	35.0	32.0	38.0	23.0	40.0
49	32.89124406957023	35.0	32.0	38.0	22.0	40.0
50	32.78717182006375	35.0	32.0	38.0	22.0	40.0
51	32.59994338129915	35.0	31.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	7.0
15	13.0
16	64.0
17	158.0
18	343.0
19	686.0
20	1146.0
21	1795.0
22	2594.0
23	3631.0
24	4836.0
25	6187.0
26	7929.0
27	9533.0
28	10532.0
29	11884.0
30	13826.0
31	16778.0
32	20667.0
33	26343.0
34	44371.0
35	62815.0
36	26725.0
37	30718.0
38	39583.0
39	52435.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.410341506815726	24.37182309689128	27.37210871801612	14.845726678276872
2	30.004625545650093	26.292309208880038	30.312995255656183	13.390069989813687
3	28.86795457360305	25.971301396004844	31.331879108963197	13.828864921428915
4	25.215037320317773	28.657151017746425	31.16328681668937	14.96452484524643
5	22.909594594936163	33.18184460694236	29.962919806182054	13.945640991939417
6	20.962315704864913	41.69891489248766	26.841055635456453	10.497713767190978
7	86.97264356252954	4.853031501735212	6.483599533906766	1.6907254018284807
8	88.05219030960825	3.958253818602782	6.0551678466442045	1.9343880251447694
9	83.53331024773209	5.534225246379815	8.172302839276192	2.7601616666119018
10	55.32683397829785	21.74562532069186	12.312545339194044	10.614995361816247
11	51.77097735504728	19.400751714358655	17.06952725912408	11.758743671469988
12	45.254013229566084	21.09223540235928	20.50633295334771	13.147418414726927
13	20.51012438420844	44.3079248487851	21.772670860831738	13.409279906174723
14	15.277949796400161	45.28889439348481	25.88055981740469	13.552595992710343
15	13.894835818405626	21.820695651734326	50.674748312181364	13.609720217678683
16	15.142216571586006	17.022260754393635	49.290623285957295	18.54489938806306
17	15.97304545420078	17.123112815289073	27.900128655887208	39.003713074622944
18	21.19713165617283	20.92237929979855	36.318368976996126	21.5621200670325
19	31.469381668179025	22.39016856701607	24.985023848100113	21.155425916704793
20	33.21950615349228	21.93266924315457	23.998493538138003	20.84933106521514
21	22.636106048848795	28.98574169234308	27.312962396588723	21.065189862219402
22	24.172393833616848	24.661235652593717	23.57006185087544	27.596308662913994
23	19.153297660181636	31.53535256515574	22.748837926441187	26.562511848221437
24	19.45206241200721	22.82314997131151	40.45203966342205	17.27274795325924
25	17.717861936309017	24.04146308789295	37.82508360105048	20.415591374747557
26	15.786507055852834	35.664473534548776	26.89084976076071	21.658169648837674
27	18.10307131175925	35.03964572870038	27.002823352180954	19.85445960735942
28	15.630805628505492	28.83231512351218	38.6890243131823	16.847854934800026
29	15.985936319127264	24.298016576135723	37.60315851466905	22.112888590067968
30	18.406638542675083	32.35758753781952	30.18029517553061	19.055478743974785
31	31.36347436613595	26.313035697585363	22.763498125769345	19.55999181050934
32	32.9391930318556	24.59450646944486	25.735474396467396	16.73082610223214
33	29.962161520009907	26.07114240867075	23.081725556013335	20.884970515306005
34	20.030634761354705	27.630431540660567	26.934830358745188	25.40410333923954
35	20.843012013780587	24.0773553000412	32.05907554805133	23.020557138126883
36	33.10348836915393	24.07710253798382	23.95476570221091	18.86464339065134
37	21.14784305498333	33.04459480978391	27.515172042494356	18.292390092738398
38	21.579307886934476	34.40217981998286	22.040598641656704	21.977913651425958
39	20.66481476332625	32.99454792242227	25.567134866250957	20.773502448000524
40	25.680372267958113	25.44631460282234	23.636791034024302	25.236522095195248
41	17.700168592292272	23.285704536320644	26.654011712993743	32.36011515839334
42	22.303218419276647	24.458267720515938	23.16134560408868	30.077168256118735
43	21.34929441471682	25.491053486978966	26.28169320246999	26.87795889583423
44	19.817303584924257	29.07092250568083	28.242621243639874	22.86915266575504
45	16.897396298046907	37.92745223429021	23.259922806467674	21.91522866119521
46	22.869658189869803	32.81837276842698	25.224389516440908	19.087579525262306
47	22.00066223659034	26.73590661958552	26.459890452924327	24.80354069089981
48	23.444691870413948	23.809680281273618	30.872357688642644	21.87327015966979
49	21.713524539404343	23.197996102409075	32.74203862709761	22.346440731088972
50	20.703992882220465	33.61027629420492	25.04138978689631	20.644341036678302
51	19.20486111988757	35.36495049655106	22.807984247868585	22.622204135692783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	626.0
1	693.5
2	761.0
3	1778.0
4	2795.0
5	2073.0
6	1351.0
7	1529.5
8	1708.0
9	1872.5
10	2037.0
11	2124.0
12	2211.0
13	2141.0
14	2071.0
15	2038.5
16	2006.0
17	1904.0
18	1802.0
19	1802.0
20	1802.0
21	1709.5
22	1617.0
23	1619.0
24	1621.0
25	1744.5
26	2267.5
27	2667.0
28	2894.5
29	3122.0
30	3454.0
31	3786.0
32	4572.5
33	5359.0
34	6416.5
35	7474.0
36	8196.0
37	8918.0
38	11318.0
39	13718.0
40	20874.5
41	28031.0
42	34808.5
43	41586.0
44	42092.0
45	42598.0
46	39126.5
47	35655.0
48	33531.0
49	31407.0
50	29285.5
51	27164.0
52	25075.0
53	22986.0
54	21237.0
55	19488.0
56	18102.0
57	16716.0
58	15660.0
59	14604.0
60	13106.0
61	11608.0
62	10855.5
63	10103.0
64	8681.5
65	7260.0
66	6319.0
67	5378.0
68	4485.0
69	3592.0
70	3445.5
71	3299.0
72	2548.5
73	1798.0
74	1540.5
75	1085.5
76	888.0
77	683.5
78	479.0
79	366.0
80	253.0
81	167.5
82	82.0
83	52.0
84	22.0
85	23.0
86	24.0
87	13.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.124947867825664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68462875318345	17.733280421809322
2	5.447649444195477	2.5195321879841974
3	2.1401479959339373	1.4847243250621163
4	1.2908655685382944	1.1940479590727677
5	1.0143295915355943	1.1728159462526762
6	0.7345145318016374	1.0191366153643944
7	0.698444621757807	1.1306046826698752
8	0.6525374635202046	1.207191586056634
9	0.5377695679261988	1.1192303900876832
>10	9.735596629102952	54.55565694122524
>50	1.0077714260730797	14.438021479719637
>100	0.05355835127720273	1.7129684628781006
>500	0.0	0.0
>1k	0.00218605515417154	0.7127890018173592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.4152880602786954	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1177	0.29750094153866374	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	481	0.12157854960076232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02123201282009155	0.0
2	0.0	0.0	0.0	0.07254271046864613	0.0
3	0.0	0.0	0.0	0.10262139529710916	0.0
4	0.0	0.0	0.0	0.19058259126605986	0.0
5	0.0	0.0	0.0	0.30457827914536095	0.0
6	0.0	0.0	0.0	0.5482409024616497	0.0
7	0.0	0.0	0.0	0.6614783041688046	0.0
8	0.0	0.0	0.0	1.04087415229925	0.0
9	0.0	0.0	0.0	1.190003766154655	0.0
10	0.0	0.0	0.0	1.435182961815236	0.0
11	0.0	0.0	0.0	1.5618167525636393	0.0
12	0.0	0.0	0.0	1.6909781638858628	0.0
13	0.0	0.0	0.0	1.746080292395148	0.0
14	0.0	0.0	0.0	1.7746424048793188	0.0
15	0.0	0.0	0.0	1.8026989932487254	0.0
16	0.0	0.0	0.0	1.8676588419959104	0.0
17	0.0	0.0	0.0	1.9217499222756673	0.0
18	0.0	0.0	0.0	2.0448450442207218	0.0
19	0.0	0.0	0.0	2.0794734460820616	0.0
20	0.0	0.0	0.0	2.122695757894391	0.0
21	0.0	0.0	0.0	2.1740064555429455	0.0
22	0.0	0.0	0.0	2.22405334290459	0.0
23	0.0	0.0	0.0	2.294321194856798	0.0
24	0.0	0.0	0.0	2.3395656031281833	0.0
25	0.0	0.0	0.0	2.3769743876207254	0.0
26	0.0	0.0	0.0	2.420702223547819	0.0
27	0.0	0.0	0.0	2.4608913906715637	0.0
28	0.0	0.0	0.0	2.5008277957379264	0.0
29	0.0	0.0	0.0	2.5448083937224015	0.0
30	0.0	0.0	0.0	2.625186727969891	0.0
31	0.0	0.0	0.0	2.6873661940858735	0.0
32	0.0	0.0	0.0	2.738171367619664	0.0
33	0.0	0.0	0.0	2.786196158522252	0.0
34	0.0	0.0	0.0	2.8364958079412785	0.0
35	0.0	0.0	0.0	2.919149000705206	0.0
36	0.0	0.0	0.0	2.983603325337627	0.0
37	2.527620573820423E-4	0.0	0.0	3.044518981166699	0.0
38	2.527620573820423E-4	0.0	0.0	3.098104537331692	0.0
39	2.527620573820423E-4	0.0	0.0	3.1620533378493487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	20	7.0276705E-4	45.000004	1
CACGGCA	20	7.0276705E-4	45.000004	35
CGACGGT	40	6.7957444E-9	45.000004	28
GGCGTAG	20	7.0276705E-4	45.000004	1
TCGATAG	30	2.161878E-6	45.000004	1
GTTGTAC	20	7.0276705E-4	45.000004	44
CTACGAA	30	2.161878E-6	45.000004	11
TACGAAT	30	2.161878E-6	45.000004	12
GGAACTA	30	2.161878E-6	45.000004	8
TATTACG	20	7.0276705E-4	45.000004	1
GTGTACG	20	7.0276705E-4	45.000004	1
CGAATAT	30	2.161878E-6	45.000004	14
AATTCGC	55	1.8189894E-12	45.000004	13
TCGTAAG	20	7.0276705E-4	45.000004	1
TGCTACG	20	7.0276705E-4	45.000004	1
ACCCGCT	120	0.0	45.000004	34
TTGCACG	20	7.0276705E-4	45.000004	1
GCTAATT	20	7.0276705E-4	45.000004	20
CAATACG	20	7.0276705E-4	45.000004	1
ATCGCGG	40	6.7957444E-9	45.000004	2
>>END_MODULE