FastQCFastQC Report
Thu 26 May 2016
SRR1545484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545484_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences548293
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.5393101863419741No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6690.12201505399485311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG302.1634169E-645.0000041
AGGTACG207.030055E-445.01
TCATTAC207.030055E-445.019
TTATACG253.8881277E-545.01
CCGTAGG207.030055E-445.02
TACGCGG406.8030204E-945.02
GCGATCG207.030055E-445.09
GCGATAT253.8881277E-545.09
ACGTAAG207.030055E-445.01
TCGACAA750.045.019
GACCTAT453.8380676E-1045.033
TAAGACG351.2103737E-745.01
ATACCGG207.030055E-445.02
AATCGCC207.030055E-445.014
TACGGGC750.042.04
CGTTTTT20500.041.9268261
ACGTAGG700.041.7857132
TTACGGG1300.041.5384643
GTTGATC3000.040.516
TATAGCG501.0786607E-940.51
Produced by FastQC (version 0.11.5)