FastQCFastQC Report
Thu 26 May 2016
SRR1545483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545483_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3186802
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269140.8445457232674011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1500.043.5000041
CGTTTTT222700.042.656041
CTAAGCG900.040.01
AACGGGA10350.038.9130444
GTTGATC22300.038.44170416
ATAACGC3700.038.3108111
AGGGTAC15900.038.2075466
TTGATCC22500.038.117
CGTTCGA2200.037.84090814
GCGATAT4350.037.758629
GGTACGA601.5643309E-1037.5000048
CATATGC22950.037.35294333
GCCAGTA22900.037.33624325
AACACGT14650.037.3208241
GTTTTTT267500.037.2448582
CGTTAGG3750.037.22
GGGCGAT69100.037.0875557
ATCCTGC23200.037.04741720
ACGGGTA2800.036.9642835
GGCGATA16200.036.9444438
Produced by FastQC (version 0.11.5)