##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545483_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3186802
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.024845911355648	31.0	31.0	33.0	30.0	34.0
2	31.32256318403214	31.0	31.0	34.0	30.0	34.0
3	31.39297797603993	31.0	31.0	34.0	28.0	34.0
4	35.20544891085169	37.0	35.0	37.0	32.0	37.0
5	35.088671966441595	37.0	35.0	37.0	32.0	37.0
6	35.13221185376437	37.0	35.0	37.0	32.0	37.0
7	35.71773552294746	37.0	35.0	37.0	35.0	37.0
8	35.7039539952592	37.0	35.0	37.0	35.0	37.0
9	37.35847567561461	39.0	37.0	39.0	35.0	39.0
10	36.5847928424797	39.0	35.0	39.0	32.0	39.0
11	36.302652941726535	38.0	35.0	39.0	32.0	39.0
12	35.75943469346385	37.0	35.0	39.0	30.0	39.0
13	35.54578226071152	37.0	35.0	39.0	30.0	39.0
14	36.513321819177975	38.0	35.0	40.0	30.0	41.0
15	36.70661151838112	38.0	35.0	40.0	31.0	41.0
16	36.748866732228734	38.0	35.0	40.0	31.0	41.0
17	36.64618354074084	38.0	35.0	40.0	31.0	41.0
18	36.510582395768544	38.0	35.0	40.0	31.0	41.0
19	36.39997370404562	38.0	35.0	40.0	30.0	41.0
20	36.25701377117248	38.0	35.0	40.0	30.0	41.0
21	36.136365233861405	38.0	34.0	40.0	30.0	41.0
22	36.0267104765216	38.0	34.0	40.0	30.0	41.0
23	35.960443416315165	37.0	34.0	40.0	30.0	41.0
24	35.84366709949347	37.0	34.0	40.0	30.0	41.0
25	35.71767558825431	37.0	34.0	40.0	29.0	41.0
26	35.56992935237269	37.0	34.0	40.0	29.0	41.0
27	35.46154734432827	37.0	34.0	40.0	29.0	41.0
28	35.41043999595833	37.0	34.0	40.0	28.0	41.0
29	35.3441864289027	37.0	34.0	40.0	28.0	41.0
30	35.198992281290145	37.0	34.0	40.0	27.0	41.0
31	35.03291073621769	37.0	34.0	40.0	27.0	41.0
32	34.80265607966858	37.0	33.0	40.0	25.0	41.0
33	34.52357975173857	37.0	33.0	40.0	24.0	41.0
34	34.25875752556952	37.0	33.0	40.0	23.0	41.0
35	34.02801397764907	36.0	33.0	40.0	23.0	41.0
36	33.848764372559074	36.0	33.0	40.0	21.0	41.0
37	33.78322876664443	36.0	33.0	40.0	22.0	41.0
38	33.67884230021194	36.0	33.0	40.0	21.0	41.0
39	33.57693449420454	36.0	32.0	40.0	21.0	41.0
40	33.47231487867775	36.0	32.0	40.0	21.0	41.0
41	33.36317003692103	35.0	32.0	40.0	20.0	41.0
42	33.30633060980883	35.0	32.0	40.0	20.0	41.0
43	33.22965436823499	35.0	32.0	40.0	20.0	41.0
44	33.09900489581719	35.0	32.0	40.0	19.0	41.0
45	33.01907492213197	35.0	31.0	39.0	20.0	41.0
46	32.93911984491035	35.0	31.0	39.0	20.0	41.0
47	32.819597201206726	35.0	31.0	39.0	20.0	41.0
48	32.71285100235283	35.0	31.0	39.0	19.0	41.0
49	32.672250111553836	35.0	31.0	39.0	18.0	41.0
50	32.62225547743474	35.0	31.0	39.0	18.0	41.0
51	32.46667442784334	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	10.0
13	7.0
14	56.0
15	177.0
16	538.0
17	1421.0
18	3171.0
19	5780.0
20	9749.0
21	14688.0
22	21000.0
23	29730.0
24	40868.0
25	56156.0
26	71202.0
27	79760.0
28	85084.0
29	93582.0
30	107124.0
31	125454.0
32	150694.0
33	186346.0
34	273544.0
35	346604.0
36	263546.0
37	314576.0
38	401671.0
39	504065.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.28029290806269	23.580065532781767	26.12455998207607	19.015081577079467
2	33.6440105158714	25.25679348764059	27.93910635175954	13.160089644728476
3	28.324477014888277	25.77220046931061	33.11219209728122	12.791130418519883
4	25.417707155951323	27.846882234917636	32.551316335310446	14.184094273820588
5	22.566635768397283	31.209595073682017	31.628353440220007	14.595415717700691
6	20.688702969309045	39.42168355611676	29.114673581854163	10.774939892720036
7	84.52759223823759	3.901058176818014	9.821884133372578	1.7494654515718264
8	85.05941065682775	3.2137861090836517	9.737347974552545	1.9894552595360488
9	79.90097910067836	5.238668734361282	11.746729166104453	3.1136229988559063
10	41.36033553386749	27.35193463541193	18.180702786053228	13.10702704466735
11	36.091793591192676	24.636485103247708	24.877855605713815	14.3938656998458
12	32.042342134842386	21.262569811365754	28.910958383984948	17.784129669806912
13	23.12923739849542	28.94393815492773	29.762784132807752	18.1640403137691
14	19.643391713699188	31.271004599595457	31.42714859599059	17.658455090714767
15	19.9623949024759	22.651830895047762	39.43900499623133	17.946769206245005
16	21.993898585478483	20.416674773016965	38.37348539382114	19.215941247683414
17	21.872271951630506	20.621739285967564	31.111063693320133	26.394925069081793
18	23.806248395727128	21.868067109283853	33.606951420263954	20.71873307472507
19	28.54654917374848	23.73244399871721	27.85645295816935	19.86455386936496
20	29.526465717041724	23.64166333521819	27.466783314432462	19.365087633307624
21	25.681043252765622	25.46028902956632	29.715746381482127	19.142921336185932
22	24.80122706085913	23.23941054386184	28.588315182430534	23.37104721284849
23	22.858119205397763	26.219608246762743	28.454638851111554	22.467633696727944
24	22.2278635447072	23.788550402566585	34.37169927720643	19.611886775519785
25	22.53086322903023	24.387269745657246	32.138206264461985	20.943660760850534
26	20.92075378388742	28.06387092765726	28.686846562792418	22.328528725662906
27	20.746409723603787	26.93461972221682	30.093868398475966	22.22510215570343
28	19.581134943432318	26.60890133745366	33.772979934115774	20.036983784998252
29	20.97183948045721	23.903210805064138	33.64096671208315	21.483983002395505
30	22.369949560719494	25.56861078912339	31.99746956353109	20.06397008662603
31	26.700592004147104	25.24524586089754	27.6604570977425	20.393705037212857
32	27.979648563042197	24.194443206700637	27.80508484681508	20.020823383442085
33	26.748163205621182	25.07633044035996	26.399286808530935	21.776219545487923
34	22.677028569707186	26.47167913161847	28.687191736417887	22.164100562256454
35	22.663190245267824	25.85595214261821	29.885979737680596	21.594877874433365
36	27.49609169317705	26.499638195281666	25.68609533946571	20.31817477207558
37	23.328308442131014	28.943310566517784	27.41350105842785	20.31487993292335
38	24.13479092833505	29.150759915426182	25.51332025020695	21.201128906031816
39	23.22805119364178	27.958404695365445	26.334958996511237	22.47858511448154
40	25.277943217055842	25.566822162155038	26.790368526190207	22.364866094598913
41	20.59742023508207	25.222087848570446	28.203321072347766	25.97717084399972
42	23.138117774496187	26.077992922057913	25.867970460668722	24.91591884277718
43	22.514043859643618	25.971553927730685	27.00741997777082	24.50698223485488
44	21.87741817659208	26.978111599026235	28.372362010567333	22.772108213814352
45	21.154153913547187	29.48620592054354	26.278130865990416	23.081509299918853
46	23.19510280211949	28.581066536295634	26.652393214263075	21.5714374473218
47	22.27182611282408	27.200403413829914	27.871923012474575	22.65584746087143
48	23.392636254150712	25.648471414289308	29.11162977806591	21.84726255349407
49	22.72758081612852	25.457370743460057	29.31408979911523	22.500958641296194
50	21.872146433948515	28.554017475826864	27.556465698214073	22.017370392010548
51	21.625629706520833	29.27106861361327	26.312742366799068	22.79055931306683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3525.0
1	4374.0
2	5223.0
3	26805.5
4	48388.0
5	35018.5
6	21649.0
7	22160.5
8	22672.0
9	23211.0
10	23750.0
11	24013.5
12	24277.0
13	23889.5
14	23502.0
15	22685.0
16	21868.0
17	21518.5
18	21169.0
19	20252.0
20	19335.0
21	18881.0
22	18427.0
23	18454.0
24	18481.0
25	19403.0
26	22849.5
27	25374.0
28	29140.0
29	32906.0
30	38306.5
31	43707.0
32	52215.5
33	60724.0
34	68800.5
35	76877.0
36	82294.0
37	87711.0
38	98646.5
39	109582.0
40	135164.0
41	160746.0
42	185806.0
43	210866.0
44	212513.0
45	214160.0
46	211161.5
47	208163.0
48	210442.0
49	212721.0
50	211302.0
51	209883.0
52	204698.5
53	199514.0
54	187527.5
55	175541.0
56	167114.5
57	158688.0
58	153314.5
59	147941.0
60	140748.5
61	133556.0
62	123889.0
63	114222.0
64	101595.0
65	88968.0
66	76812.5
67	64657.0
68	56903.5
69	49150.0
70	42717.0
71	36284.0
72	32233.0
73	28182.0
74	23078.0
75	15288.0
76	12602.0
77	9799.5
78	6997.0
79	5519.5
80	4042.0
81	2817.5
82	1593.0
83	1054.0
84	515.0
85	369.0
86	223.0
87	145.5
88	68.0
89	53.5
90	39.0
91	29.5
92	20.0
93	13.0
94	6.0
95	5.0
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3186802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.440696268808964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26151716083895	15.603397771976683
2	5.863144380519861	2.279672181637214
3	2.2384587267042906	1.305515886483689
4	1.3063516702810438	1.0158554416874017
5	0.9351488933779002	0.9089972801136288
6	0.6645709063329539	0.7751832683463628
7	0.5253275608958562	0.7148913487107409
8	0.42888305538491006	0.6670228171661448
9	0.34153616921623553	0.5975730837490839
>10	4.521339376614091	21.611830830563814
>50	1.9614586821288051	27.509112370794654
>100	0.9469010742876859	25.307256157699886
>500	0.004387371887055265	0.5413614114714846
>1k	8.124762753806046E-4	0.31211126482688145
>5k	0.0	0.0
>10k+	1.6249525507612092E-4	0.8502188847723368
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26914	0.8445457232674011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01622316039716305	0.0
2	0.0	0.0	0.0	0.058553998648174566	0.0
3	0.0	0.0	0.0	0.10502692040484474	0.0
4	0.0	0.0	0.0	0.15319433086837525	0.0
5	0.0	0.0	0.0	0.2544557208135303	0.0
6	0.0	0.0	0.0	0.4977403679299812	0.0
7	0.0	0.0	0.0	0.6366570624720331	0.0
8	0.0	0.0	0.0	0.9352950073459223	0.0
9	0.0	0.0	0.0	1.1117414888028814	0.0
10	0.0	0.0	0.0	1.3473381778974658	0.0
11	3.137942049741402E-5	0.0	0.0	1.486945219690461	0.0
12	3.137942049741402E-5	0.0	0.0	1.6143456669099618	0.0
13	3.137942049741402E-5	0.0	0.0	1.6847297070856615	0.0
14	3.137942049741402E-5	0.0	0.0	1.7194039667353038	0.0
15	3.137942049741402E-5	0.0	0.0	1.7481161364904378	0.0
16	3.137942049741402E-5	0.0	0.0	1.7948400936110873	0.0
17	3.137942049741402E-5	0.0	0.0	1.8451099252479446	0.0
18	3.137942049741402E-5	0.0	0.0	1.9301795342164338	0.0
19	3.137942049741402E-5	0.0	0.0	1.966956215039403	0.0
20	6.275884099482804E-5	0.0	0.0	2.0074356674810674	0.0
21	6.275884099482804E-5	0.0	0.0	2.0528730683613228	0.0
22	9.413826149224207E-5	0.0	0.0	2.1024839321677344	0.0
23	9.413826149224207E-5	0.0	0.0	2.157429297458706	0.0
24	9.413826149224207E-5	0.0	0.0	2.204780842989304	0.0
25	9.413826149224207E-5	0.0	0.0	2.248680652265186	0.0
26	9.413826149224207E-5	0.0	0.0	2.2917332171876383	0.0
27	2.1965594348189815E-4	0.0	0.0	2.3412499427325577	0.0
28	2.1965594348189815E-4	0.0	0.0	2.390170459288026	0.0
29	2.1965594348189815E-4	0.0	0.0	2.445868930670936	0.0
30	2.1965594348189815E-4	0.0	0.0	2.5366495941699547	0.0
31	2.1965594348189815E-4	0.0	0.0	2.596458769638026	0.0
32	2.1965594348189815E-4	0.0	0.0	2.6621045173186157	0.0
33	2.1965594348189815E-4	0.0	0.0	2.7244554258469775	0.0
34	2.1965594348189815E-4	0.0	0.0	2.785959090021909	0.0
35	2.1965594348189815E-4	0.0	0.0	2.856280371356614	0.0
36	2.5103536397931217E-4	0.0	0.0	2.921173012945266	0.0
37	2.5103536397931217E-4	0.0	0.0	2.988827043537691	0.0
38	2.5103536397931217E-4	0.0	0.0	3.0636042025830283	0.0
39	2.5103536397931217E-4	0.0	0.0	3.1768525311581954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	150	0.0	43.500004	1
CGTTTTT	22270	0.0	42.65604	1
CTAAGCG	90	0.0	40.0	1
AACGGGA	1035	0.0	38.913044	4
GTTGATC	2230	0.0	38.441704	16
ATAACGC	370	0.0	38.31081	11
AGGGTAC	1590	0.0	38.207546	6
TTGATCC	2250	0.0	38.1	17
CGTTCGA	220	0.0	37.840908	14
GCGATAT	435	0.0	37.75862	9
GGTACGA	60	1.5643309E-10	37.500004	8
CATATGC	2295	0.0	37.352943	33
GCCAGTA	2290	0.0	37.336243	25
AACACGT	1465	0.0	37.32082	41
GTTTTTT	26750	0.0	37.244858	2
CGTTAGG	375	0.0	37.2	2
GGGCGAT	6910	0.0	37.087555	7
ATCCTGC	2320	0.0	37.047417	20
ACGGGTA	280	0.0	36.964283	5
GGCGATA	1620	0.0	36.944443	8
>>END_MODULE