FastQCFastQC Report
Thu 26 May 2016
SRR1545482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545482_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1316598
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.5280275376386718No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29990.22778403126846616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15740.11955053858505026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG551.8189894E-1245.0000041
ACGTTAC253.8911152E-545.00000445
GCGATAC502.1827873E-1145.0000049
TTACGAG453.8562575E-1045.0000041
CTTAACG207.0336607E-445.01
AACGCAT207.0336607E-445.042
ACGTGCG351.2121018E-745.015
TATCGCC207.0336607E-445.032
CCGTTGT207.0336607E-445.029
TCGAATA302.1657434E-644.99999612
AATGCGG1950.041.5384642
CGTTTTT52600.041.449621
CGTTAGG2800.040.982142
CGTTCGA556.184564E-1140.90909214
TGCGTAG1550.040.645161
TACGTTG451.9286745E-840.0000041
TCGAGTA403.4587356E-739.37524
AGGGTAC6450.039.0697676
AGGGCGA14000.039.0535746
TCGTTAG750.039.01
Produced by FastQC (version 0.11.5)