##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545481_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1412973
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.932811171904913	31.0	31.0	33.0	28.0	34.0
2	31.214855485561294	31.0	31.0	34.0	28.0	34.0
3	31.29171682686081	31.0	31.0	34.0	28.0	34.0
4	35.105928421845285	35.0	35.0	37.0	32.0	37.0
5	34.960610712306604	35.0	35.0	37.0	32.0	37.0
6	34.99466656475389	37.0	35.0	37.0	32.0	37.0
7	35.57429759804327	37.0	35.0	37.0	35.0	37.0
8	35.5253221399135	37.0	35.0	37.0	35.0	37.0
9	37.27926577507143	39.0	37.0	39.0	35.0	39.0
10	36.681334321321074	39.0	37.0	39.0	32.0	39.0
11	36.218389169502885	38.0	35.0	39.0	32.0	39.0
12	34.79734715383804	35.0	33.0	39.0	30.0	39.0
13	34.00586352322373	35.0	33.0	39.0	25.0	39.0
14	34.849837187264015	35.0	33.0	40.0	25.0	41.0
15	35.45341772277319	35.0	33.0	40.0	30.0	41.0
16	35.70707083574845	35.0	34.0	40.0	31.0	41.0
17	35.651340117610175	35.0	34.0	40.0	31.0	41.0
18	35.619793159529586	36.0	34.0	39.0	30.0	41.0
19	35.46805282195768	36.0	34.0	39.0	30.0	41.0
20	35.249389054143286	35.0	34.0	39.0	30.0	41.0
21	34.987505776826595	35.0	34.0	39.0	29.0	41.0
22	34.872023740014846	35.0	33.0	39.0	29.0	41.0
23	34.929077908778154	35.0	34.0	39.0	29.0	41.0
24	34.829260714819036	35.0	34.0	39.0	29.0	41.0
25	34.70983946614691	35.0	34.0	39.0	29.0	41.0
26	34.51781244227597	35.0	34.0	39.0	28.0	41.0
27	34.354601963377924	35.0	33.0	39.0	27.0	41.0
28	34.47624547673593	35.0	33.0	39.0	27.0	41.0
29	34.61810169054893	35.0	34.0	39.0	29.0	41.0
30	34.53180917115897	35.0	34.0	39.0	28.0	41.0
31	34.250957378520326	35.0	33.0	39.0	27.0	41.0
32	33.911679133288466	35.0	33.0	39.0	24.0	41.0
33	33.60361662961713	35.0	33.0	39.0	23.0	41.0
34	33.41914318249535	35.0	33.0	39.0	23.0	41.0
35	33.18916355797315	35.0	32.0	38.0	23.0	41.0
36	32.91705927855663	35.0	32.0	38.0	21.0	41.0
37	32.86519841497325	35.0	32.0	38.0	21.0	41.0
38	32.92929376569828	35.0	32.0	38.0	23.0	41.0
39	32.90273841042964	35.0	32.0	38.0	22.0	41.0
40	32.655512171853246	35.0	31.0	38.0	20.0	41.0
41	32.68906624542719	35.0	32.0	38.0	20.0	40.0
42	32.657602091476626	35.0	32.0	38.0	21.0	40.0
43	32.46820073702753	35.0	31.0	38.0	20.0	40.0
44	32.39766931144474	35.0	31.0	38.0	19.0	40.0
45	32.35684758307484	35.0	31.0	38.0	20.0	40.0
46	32.32463819195413	35.0	31.0	38.0	20.0	40.0
47	32.255479757928846	35.0	31.0	38.0	20.0	40.0
48	32.269745423302496	35.0	31.0	37.0	20.0	40.0
49	32.33839712436119	35.0	31.0	37.0	19.0	40.0
50	32.165340031267405	35.0	31.0	37.0	18.0	40.0
51	31.97166258661701	35.0	31.0	37.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	6.0
14	18.0
15	84.0
16	217.0
17	616.0
18	1364.0
19	2775.0
20	4601.0
21	7084.0
22	10299.0
23	14411.0
24	19961.0
25	27575.0
26	34968.0
27	40087.0
28	42639.0
29	46209.0
30	53408.0
31	63282.0
32	77480.0
33	99997.0
34	173529.0
35	243222.0
36	81766.0
37	92768.0
38	119694.0
39	154831.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.6682951478903	24.284894332729642	28.517388513439396	17.529422005940663
2	30.314308907530435	26.45591953986382	30.017275630886083	13.212495921719663
3	26.91219152807591	25.12001290895155	33.790242276391695	14.177553286580846
4	23.671365270249325	28.36069762125674	32.81506440675087	15.152872701743062
5	22.044582592873326	31.76607054770332	32.406705577530495	13.782641281892861
6	20.80372377957682	38.63251456326483	30.373899572037118	10.18986208512123
7	84.4379899686689	5.3064708242832666	8.554798994743706	1.7007402123041278
8	86.74829596885432	3.6297225778553446	8.111619967260522	1.510361486029811
9	83.95383351274228	4.203123485020591	9.620424452555003	2.2226185496821245
10	62.522001481981604	15.404469866020086	13.166776718309551	8.906751933688755
11	57.49635697214314	15.754582713187018	17.37888834393863	9.370171970731217
12	49.47214136434312	19.513748670356758	20.657578028737987	10.356531936562128
13	19.00807729517832	49.258053763235395	21.56672491264872	10.167144028937566
14	11.993010482153586	49.45933149465701	27.98114330563995	10.56651471754945
15	10.878622592222214	19.9052635825313	58.57224448025546	10.643869344991023
16	12.051398009728425	14.237002405566134	56.34665347462408	17.364946110081366
17	12.493515445801158	14.242310362618394	30.160661244057742	43.1035129475227
18	20.562459438361525	20.086795713718523	39.03599007199713	20.314754775922825
19	32.10684139045827	20.404423863725636	26.942977678979002	20.545757066837087
20	34.522244940278405	20.15042042558492	25.653356433562426	19.67397820057425
21	20.332660284379106	29.322924075690054	30.262290928418306	20.082124711512535
22	21.840898587588015	24.67124283337332	24.82991536285548	28.657943216183185
23	17.07838720202014	31.778172689782462	24.247101678517566	26.896338429679833
24	18.36800844743672	21.768498053395216	45.05769041588197	14.805803083286092
25	14.256606460279142	22.713455954218517	43.47875012473699	19.55118746076535
26	13.906847476915695	36.20805210007552	29.990028118017825	19.895072304990965
27	16.183111779205973	37.885508074110405	29.590657429405944	16.340722717277682
28	12.642209016025078	29.156254224249157	43.53699610679043	14.664540652935335
29	12.487712079424023	23.479358770478985	42.42260821685907	21.61032093323793
30	15.624573151787047	32.93559041821747	34.34382681056184	17.096009619433634
31	32.28455179256787	26.018685424279163	24.98653548227744	16.71022730087553
32	34.18954219224288	24.03301407740983	27.448436735875347	14.329006994471941
33	31.900326474745093	25.560431798767564	24.265573369059425	18.27366835742792
34	18.829588392701062	26.332987254533524	29.308344886986514	25.529079465778892
35	19.116784255608565	24.227002214479683	33.918057882210064	22.738155647701692
36	35.35877897171425	22.3059463981265	25.7766425826962	16.558632047463046
37	19.768176745061652	34.25118526680977	29.980403022563063	16.000234965565514
38	19.473478969520293	35.759635888300764	23.008436820802665	21.758448321376274
39	20.642220339666785	34.4259232129701	26.86916168957227	18.062694757790844
40	26.15357830616721	24.96056187910172	23.719561520283825	25.16629829444724
41	15.511973689518483	22.424915408857775	27.52876381926618	34.534347082357556
42	21.807776935581927	23.078147990088983	23.744119668245606	31.369955406083484
43	21.88633469995534	24.788867161651353	26.101701872576477	27.223096265816828
44	17.720013050497073	30.215227042554954	30.199869353483756	21.86489055346422
45	14.898869263602348	41.71735765651573	22.49002634869881	20.893746731183114
46	21.665240595538627	35.066204378993795	25.052212604204044	18.216342421263533
47	21.02078383663382	27.86210352214798	26.466747772250425	24.650364868967774
48	23.49804277930293	22.746223742421122	33.43475069941181	20.32098277886414
49	21.709332025452717	22.092566524625738	34.65551004867043	21.542591401251123
50	19.23900881333189	36.54592125964191	25.820309376046108	18.394760550980095
51	17.202097987718094	37.31019630240635	22.39922489672485	23.08848081315071
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1348.0
1	1666.5
2	1985.0
3	7984.0
4	13983.0
5	10634.5
6	7286.0
7	8109.0
8	8932.0
9	9904.5
10	10877.0
11	11283.0
12	11689.0
13	11418.5
14	11148.0
15	10701.0
16	10254.0
17	9538.5
18	8823.0
19	7969.5
20	7116.0
21	6896.0
22	6676.0
23	6336.0
24	5996.0
25	6376.5
26	6208.5
27	5660.0
28	7201.0
29	8742.0
30	10875.0
31	13008.0
32	16066.0
33	19124.0
34	20465.5
35	21807.0
36	23552.0
37	25297.0
38	39711.0
39	54125.0
40	87182.5
41	120240.0
42	143789.5
43	167339.0
44	167262.0
45	167185.0
46	154805.0
47	142425.0
48	127647.5
49	112870.0
50	105879.0
51	98888.0
52	88574.0
53	78260.0
54	73025.5
55	67791.0
56	62362.0
57	56933.0
58	50951.5
59	44970.0
60	39406.0
61	33842.0
62	28535.0
63	23228.0
64	19809.0
65	16390.0
66	12991.5
67	9593.0
68	7734.5
69	5876.0
70	4395.5
71	2915.0
72	2341.0
73	1767.0
74	1383.0
75	715.5
76	432.0
77	310.0
78	188.0
79	129.0
80	70.0
81	75.0
82	80.0
83	49.0
84	18.0
85	18.0
86	18.0
87	17.0
88	16.0
89	11.5
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1412973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.78945163387836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56920836322723	14.866503902591338
2	6.4269616280190025	2.2866424606887237
3	2.054713767452161	1.096566935626626
4	0.9512642764176291	0.6768987934547085
5	0.550551188016037	0.48970018655927805
6	0.38683241777826766	0.4128921951889632
7	0.2772537528979886	0.3452534559243021
8	0.22676705014591314	0.3227249176582393
9	0.18823254221281882	0.30136983350552166
>10	2.55119613487	11.184546041385484
>50	1.0853432068623754	14.017205173990789
>100	1.7208901488833726	51.82205919577299
>500	0.009587131748653886	1.0387194452148774
>1k	3.9946382286057863E-4	0.07120454840974941
>5k	7.989276457211573E-4	1.067712914028428
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8226	0.5821767294916463	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6799	0.4811839999773527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02066564612345742	0.0
2	0.0	0.0	0.0	0.08959831504211332	0.0
3	0.0	0.0	0.0	0.1285941061860347	0.0
4	0.0	0.0	0.0	0.211681327244045	0.0
5	0.0	0.0	0.0	0.34020466066938293	0.0
6	0.0	0.0	0.0	0.49045523162862986	0.0
7	0.0	0.0	0.0	0.5748871351398788	0.0
8	0.0	0.0	0.0	0.76604436178186	0.0
9	0.0	0.0	0.0	0.8415588974453156	0.0
10	1.4154552139354395E-4	0.0	0.0	0.9598909533303184	0.0
11	1.4154552139354395E-4	0.0	0.0	1.1035596575447655	0.0
12	1.4154552139354395E-4	0.0	0.0	1.2224578955153425	0.0
13	1.4154552139354395E-4	0.0	0.0	1.2719280552423862	0.0
14	1.4154552139354395E-4	0.0	0.0	1.2921690648016628	0.0
15	1.4154552139354395E-4	0.0	0.0	1.3213982149694297	0.0
16	1.4154552139354395E-4	0.0	0.0	1.3896231562811179	0.0
17	1.4154552139354395E-4	0.0	0.0	1.4601835986957996	0.0
18	1.4154552139354395E-4	0.0	0.0	1.5778786997345313	0.0
19	1.4154552139354395E-4	0.0	0.0	1.6222532206914073	0.0
20	1.4154552139354395E-4	0.0	0.0	1.6699540614010318	0.0
21	1.4154552139354395E-4	0.0	0.0	1.737188184062965	0.0
22	1.4154552139354395E-4	0.0	0.0	1.8000343955616986	0.0
23	1.4154552139354395E-4	0.0	0.0	1.8773890230032704	0.0
24	1.4154552139354395E-4	0.0	0.0	1.9308224573293333	0.0
25	1.4154552139354395E-4	0.0	0.0	1.9816372995096156	0.0
26	1.4154552139354395E-4	0.0	0.0	2.0285596398515753	0.0
27	1.4154552139354395E-4	0.0	0.0	2.0723679787228773	0.0
28	2.1231828209031595E-4	0.0	0.0	2.1178040910902047	0.0
29	2.1231828209031595E-4	0.0	0.0	2.1669203870137648	0.0
30	2.1231828209031595E-4	0.0	0.0	2.24243492267722	0.0
31	2.1231828209031595E-4	0.0	0.0	2.305847316261528	0.0
32	2.1231828209031595E-4	0.0	0.0	2.364517934879152	0.0
33	2.1231828209031595E-4	0.0	0.0	2.42354241730026	0.0
34	2.1231828209031595E-4	0.0	0.0	2.488016402295019	0.0
35	2.1231828209031595E-4	0.0	0.0	2.5806579460470935	0.0
36	2.1231828209031595E-4	0.0	0.0	2.6541908444110396	0.0
37	2.1231828209031595E-4	0.0	0.0	2.7246805140650245	0.0
38	2.1231828209031595E-4	0.0	0.0	2.7897914539060547	0.0
39	2.1231828209031595E-4	0.0	0.0	2.8587948955854077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	35	1.2121927E-7	45.000004	9
TACCCGC	25	3.8912607E-5	45.000004	24
ACCGGAT	25	3.8912607E-5	45.000004	11
TACCAGA	30	2.1658561E-6	45.000004	27
GACACGC	30	2.1658561E-6	45.000004	35
CTATATG	70	0.0	45.000004	1
TTAGATC	35	1.2121927E-7	45.000004	42
CTATACG	30	2.1658561E-6	45.000004	1
CACGCTA	30	2.1658561E-6	45.000004	37
ACCGACA	35	1.2121927E-7	45.000004	13
TTCGTCA	25	3.8912607E-5	45.000004	34
CGCGATA	25	3.8912607E-5	45.000004	11
TCGATTG	25	3.8912607E-5	45.000004	1
AGGCGAT	30	2.1658561E-6	45.000004	6
TGACCGG	60	0.0	45.000004	2
CGTAGGT	25	3.8912607E-5	45.000004	16
TACGACT	30	2.1658561E-6	45.000004	32
CTCATAG	25	3.8912607E-5	45.000004	25
ATAAGTC	25	3.8912607E-5	45.000004	12
TACCTGC	25	3.8912607E-5	45.000004	40
>>END_MODULE