FastQCFastQC Report
Thu 26 May 2016
SRR1545480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545480_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1771633
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116850.6595609813093344No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32210.18180966373961197No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25630.1446687886260868No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19310.10899548608543644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA302.1661708E-645.00000427
GTACCGA253.891664E-545.036
ATCTACG253.891664E-545.01
CGGATTA207.0343236E-445.020
GGACGTA207.0343236E-445.09
CTAACGG1150.043.043482
AATACGG1150.043.043482
ACGGGTA1000.042.755
CGTTTTT92200.040.387741
GCGATAT1300.039.8076939
CGGTCTA1200.039.37500431
GGTACCT12700.038.976388
ACCGCGA356.250204E-638.5714325
CAAGCGT700.038.5714317
GGACTAA14450.038.30458
AGTACGG1950.038.0769272
TTGGGAC6150.038.048785
GTTGATC12800.037.9687516
AGGGCGA19750.037.936716
TAGTAGG6250.037.82
Produced by FastQC (version 0.11.5)