##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545480_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1771633
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.931356550707736	31.0	31.0	33.0	28.0	34.0
2	31.216367046673888	31.0	31.0	34.0	28.0	34.0
3	31.30507277748834	31.0	31.0	34.0	28.0	34.0
4	35.1490506216581	35.0	35.0	37.0	32.0	37.0
5	35.015530304526955	35.0	35.0	37.0	32.0	37.0
6	35.073444669409525	37.0	35.0	37.0	32.0	37.0
7	35.570396351840365	37.0	35.0	37.0	35.0	37.0
8	35.48344267689753	37.0	35.0	37.0	35.0	37.0
9	37.158182874218305	39.0	37.0	39.0	34.0	39.0
10	36.556155817824575	39.0	35.0	39.0	32.0	39.0
11	36.25076412552713	38.0	35.0	39.0	32.0	39.0
12	35.562200523471844	37.0	35.0	39.0	30.0	39.0
13	35.138549010997195	37.0	34.0	39.0	30.0	39.0
14	36.16794956969079	38.0	35.0	40.0	30.0	41.0
15	36.48526020908393	38.0	35.0	40.0	31.0	41.0
16	36.56623070353736	38.0	35.0	40.0	31.0	41.0
17	36.45616840508164	38.0	35.0	40.0	31.0	41.0
18	36.39089755045204	38.0	35.0	40.0	31.0	41.0
19	36.29291732542801	37.0	35.0	40.0	31.0	41.0
20	36.14772359738163	37.0	34.0	40.0	30.0	41.0
21	35.99266721719453	37.0	34.0	40.0	30.0	41.0
22	35.87051099183635	37.0	34.0	40.0	30.0	41.0
23	35.79664862869454	37.0	34.0	40.0	30.0	41.0
24	35.68724560899464	37.0	34.0	40.0	30.0	41.0
25	35.550142721432714	36.0	34.0	40.0	29.0	41.0
26	35.38910485410918	36.0	34.0	40.0	29.0	41.0
27	35.29820002223937	36.0	34.0	40.0	29.0	41.0
28	35.33264507942672	36.0	34.0	40.0	29.0	41.0
29	35.355518326876954	36.0	34.0	40.0	29.0	41.0
30	35.23004990311199	36.0	34.0	40.0	29.0	41.0
31	35.08672168558612	36.0	34.0	40.0	27.0	41.0
32	34.877948762525875	36.0	34.0	40.0	27.0	41.0
33	34.64125527126668	36.0	33.0	40.0	25.0	41.0
34	34.466898618393316	36.0	33.0	40.0	24.0	41.0
35	34.277936796164894	36.0	33.0	40.0	24.0	41.0
36	34.10005288905772	36.0	33.0	40.0	23.0	41.0
37	34.049993424145974	36.0	33.0	40.0	23.0	41.0
38	34.01654744521015	36.0	33.0	40.0	23.0	41.0
39	33.96962237664347	36.0	33.0	40.0	23.0	41.0
40	33.797301134038484	35.0	33.0	40.0	23.0	41.0
41	33.71886163782228	35.0	33.0	40.0	23.0	41.0
42	33.68110438222815	35.0	33.0	40.0	23.0	41.0
43	33.58377327584212	35.0	32.0	40.0	23.0	41.0
44	33.498524242887775	35.0	32.0	40.0	22.0	41.0
45	33.448894889630076	35.0	32.0	40.0	23.0	41.0
46	33.3822648370176	35.0	32.0	39.0	22.0	41.0
47	33.305108337900684	35.0	32.0	39.0	22.0	41.0
48	33.25318336246841	35.0	32.0	39.0	22.0	41.0
49	33.262218529458416	35.0	32.0	39.0	22.0	41.0
50	33.16727561520924	35.0	32.0	39.0	22.0	41.0
51	32.979703471317144	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	6.0
13	10.0
14	31.0
15	83.0
16	215.0
17	602.0
18	1353.0
19	2590.0
20	4402.0
21	6986.0
22	10275.0
23	14355.0
24	20086.0
25	27433.0
26	35776.0
27	41899.0
28	45741.0
29	51177.0
30	59359.0
31	71247.0
32	86747.0
33	109908.0
34	171432.0
35	220884.0
36	135902.0
37	160754.0
38	213136.0
39	279133.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.88152794625072	23.87622041359582	26.957106804851794	16.28514483530167
2	32.863860630277266	25.50539530478378	28.71571030794753	12.91503375699143
3	29.20023503739206	25.10965871599818	32.45271452947648	13.23739171713329
4	27.271506006040752	27.191297520423248	30.89861161990096	14.638584853635036
5	23.15107022729877	31.520241494711378	30.69399813618283	14.634690141807022
6	21.70472101163164	40.50878483297613	27.981924021510096	9.80457013388213
7	84.17234269174259	4.873469843923657	8.984648626436739	1.9695388378970138
8	84.60787307529269	4.467742472622716	8.895634705381985	2.0287497467026183
9	79.32472470314111	6.3026597495079395	10.820073909212574	3.5525416381383725
10	46.23282587307868	24.578340999518524	17.022769388468152	12.166063738934644
11	40.78231778252042	22.640242081740407	22.964575620345748	13.612864515393424
12	37.73778203499257	21.214100211499787	26.701410506577826	14.34670724692981
13	22.058123776199697	34.93912113852022	25.77164683656265	17.231108248717426
14	15.914752095947637	37.446807549870655	29.477719143863318	17.16072121031839
15	16.329115567388957	23.607541742561803	43.9920683346946	16.071274355354635
16	17.925439410984104	19.22858741059802	43.64555187219927	19.20042130621861
17	17.73629188438012	20.094229448198355	30.764215839284997	31.405262828136525
18	21.098669984133284	23.454801304784908	34.16926643384945	21.27726227723236
19	29.3466536240858	23.714222979590016	27.37570365871487	19.563419737609316
20	31.51104094358143	22.184165682170065	27.10008224050918	19.204711133739323
21	23.93136727527654	27.3713573860952	28.415817497190442	20.281457841437813
22	23.78709360234315	25.265108518524997	26.332936900588326	24.614860978543522
23	21.380839033817953	29.17466540756466	26.411056917544435	23.033438641072955
24	20.579205738434542	23.30048040423722	37.56562448317457	18.554689374153675
25	19.483719257882417	24.909730175493458	34.79044474786821	20.81610581875592
26	18.315475044775077	32.03011007358747	28.78733913852361	20.867075743113837
27	19.74477784055727	31.76747102814183	29.212652959162533	19.27509817213836
28	17.013963953030906	28.825496025418357	36.35911049297456	17.80142952857618
29	18.44721790574007	25.501500592955768	34.87872488263653	21.172556618667638
30	19.388214150447638	29.75808195038137	31.1771681832524	19.676535715918593
31	26.887792223332934	27.55677953616804	26.720263169629376	18.835165070869646
32	28.856202159250817	26.042357531159105	27.641560074801045	17.459880234789033
33	26.99063519363209	27.123111840883524	26.12256601677661	19.76368694870777
34	20.807131048021795	27.913907677267247	28.191730454332248	23.087230820378714
35	21.565188727010618	25.758495128505736	30.40533790011814	22.27097824436551
36	27.301534798685733	28.426880736585964	25.095378105962123	19.17620635876618
37	21.630721486899375	32.755824710874094	28.02945079483166	17.584003007394873
38	21.367630880662077	31.82239210942673	25.9080181956421	20.901958814269094
39	21.875693216371562	30.90566725727055	26.67036570215163	20.548273824206255
40	23.950615054020783	26.756500923159592	27.455291248243853	21.83759277457577
41	17.920246461880083	26.726133459920874	28.86732184374529	26.48629823445375
42	21.654371983362243	27.201796308829202	25.243659380921446	25.900172326887116
43	22.048753889772883	27.55395728122021	26.731044183530113	23.6662446454768
44	20.23861601132966	29.404340515219573	28.448273429090566	21.908770044360203
45	18.300291313155714	34.24456419585772	24.948564403575684	22.506580087410878
46	20.640448670802588	33.010279216971014	25.991895612691795	20.35737649953461
47	22.190543978352174	28.50342029077128	27.055603502531277	22.25043222834526
48	22.436644609803498	26.132331018896128	30.353747079671695	21.077277291628686
49	20.797140265506457	27.555029738100384	30.33517664211493	21.312653354278225
50	19.60744691479556	32.57813553935832	27.277206961035382	20.537210584810737
51	18.931629744986687	32.20593655683767	25.762446285432706	23.099987412742934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1694.0
1	2740.5
2	3787.0
3	12771.5
4	21756.0
5	16288.0
6	10820.0
7	10965.0
8	11110.0
9	11272.0
10	11434.0
11	11625.0
12	11816.0
13	11615.0
14	11414.0
15	10877.5
16	10341.0
17	10020.0
18	9699.0
19	9593.5
20	9488.0
21	9459.0
22	9430.0
23	9116.0
24	8802.0
25	10760.5
26	15174.0
27	17629.0
28	20387.5
29	23146.0
30	26027.5
31	28909.0
32	33466.0
33	38023.0
34	42377.0
35	46731.0
36	48198.0
37	49665.0
38	58927.5
39	68190.0
40	88156.5
41	108123.0
42	126727.0
43	145331.0
44	151234.0
45	157137.0
46	152351.0
47	147565.0
48	135688.0
49	123811.0
50	119953.5
51	116096.0
52	109966.0
53	103836.0
54	95994.5
55	88153.0
56	86320.0
57	84487.0
58	84011.5
59	83536.0
60	76073.0
61	68610.0
62	58976.5
63	49343.0
64	40086.5
65	30830.0
66	24752.0
67	18674.0
68	14645.0
69	10616.0
70	8930.5
71	7245.0
72	6033.5
73	4822.0
74	4011.5
75	2390.5
76	1580.0
77	1350.5
78	1121.0
79	807.0
80	493.0
81	368.5
82	244.0
83	161.5
84	79.0
85	77.0
86	75.0
87	45.0
88	15.0
89	8.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1771633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.890994433421444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67342161299534	15.847835857957302
2	6.502499369394721	2.5868235751991366
3	2.4097047712758903	1.4379427257491348
4	1.2722104664318017	1.0122212522374188
5	0.8172843199077996	0.8128298928904336
6	0.5655060630634909	0.6749086771477185
7	0.4746402466537014	0.6608746552846588
8	0.3935693775457122	0.6262789038341523
9	0.33727422393508677	0.6037847739746414
>10	4.800844273243987	22.800492943673202
>50	1.8421088183212186	26.60972459416961
>100	0.9046678923086436	24.40903427284864
>500	0.004274021538134674	0.5900008980461194
>1k	0.0017096086152538696	0.6649837211119232
>5k	0.0	0.0
>10k+	2.849347692089783E-4	0.6622632558759757
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11685	0.6595609813093344	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3221	0.18180966373961197	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2563	0.1446687886260868	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1931	0.10899548608543644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013829049244397682	0.0
2	0.0	0.0	0.0	0.050744143962095985	0.0
3	0.0	0.0	0.0	0.07727334047175685	0.0
4	0.0	0.0	0.0	0.11655912934563761	0.0
5	0.0	0.0	0.0	0.18982486779146696	0.0
6	5.644509895672524E-5	0.0	0.0	0.30277151080387416	0.0
7	5.644509895672524E-5	0.0	0.0	0.37140875113525207	0.0
8	5.644509895672524E-5	0.0	0.0	0.541308498994995	0.0
9	5.644509895672524E-5	0.0	0.0	0.6329753397007168	0.0
10	5.644509895672524E-5	0.0	0.0	0.7723382890248714	0.0
11	5.644509895672524E-5	0.0	0.0	0.8929050203964365	0.0
12	5.644509895672524E-5	0.0	0.0	0.9965946671799407	0.0
13	5.644509895672524E-5	0.0	0.0	1.0433312091161093	0.0
14	5.644509895672524E-5	0.0	0.0	1.0634821094436602	0.0
15	5.644509895672524E-5	0.0	0.0	1.0918739942188929	0.0
16	5.644509895672524E-5	0.0	0.0	1.1463999598110894	0.0
17	5.644509895672524E-5	0.0	0.0	1.2060059843093913	0.0
18	5.644509895672524E-5	0.0	0.0	1.2971648191245027	0.0
19	5.644509895672524E-5	0.0	0.0	1.3375230648785612	0.0
20	5.644509895672524E-5	0.0	0.0	1.3805906753825425	0.0
21	5.644509895672524E-5	0.0	0.0	1.437995341021532	0.0
22	5.644509895672524E-5	0.0	0.0	1.5005929557645403	0.0
23	5.644509895672524E-5	0.0	0.0	1.5686093000073944	0.0
24	5.644509895672524E-5	0.0	0.0	1.6256752950526436	0.0
25	5.644509895672524E-5	0.0	0.0	1.6732020683742062	0.0
26	5.644509895672524E-5	0.0	0.0	1.7208417318936822	0.0
27	5.644509895672524E-5	0.0	0.0	1.7649253541788847	0.0
28	5.644509895672524E-5	0.0	0.0	1.8123392373025338	0.0
29	5.644509895672524E-5	0.0	0.0	1.8629704910667164	0.0
30	5.644509895672524E-5	0.0	0.0	1.932736633377229	0.0
31	5.644509895672524E-5	0.0	0.0	1.99476979713067	0.0
32	5.644509895672524E-5	0.0	0.0	2.0565771804882838	0.0
33	5.644509895672524E-5	0.0	0.0	2.1162396500855425	0.0
34	5.644509895672524E-5	0.0	0.0	2.1803048374014256	0.0
35	5.644509895672524E-5	0.0	0.0	2.2616422249980666	0.0
36	5.644509895672524E-5	0.0	0.0	2.3323679339908434	0.0
37	5.644509895672524E-5	0.0	0.0	2.402303411598226	0.0
38	5.644509895672524E-5	0.0	0.0	2.47359357158057	0.0
39	5.644509895672524E-5	0.0	0.0	2.542456592307775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGTA	30	2.1661708E-6	45.000004	27
GTACCGA	25	3.891664E-5	45.0	36
ATCTACG	25	3.891664E-5	45.0	1
CGGATTA	20	7.0343236E-4	45.0	20
GGACGTA	20	7.0343236E-4	45.0	9
CTAACGG	115	0.0	43.04348	2
AATACGG	115	0.0	43.04348	2
ACGGGTA	100	0.0	42.75	5
CGTTTTT	9220	0.0	40.38774	1
GCGATAT	130	0.0	39.807693	9
CGGTCTA	120	0.0	39.375004	31
GGTACCT	1270	0.0	38.97638	8
ACCGCGA	35	6.250204E-6	38.57143	25
CAAGCGT	70	0.0	38.57143	17
GGACTAA	1445	0.0	38.3045	8
AGTACGG	195	0.0	38.076927	2
TTGGGAC	615	0.0	38.04878	5
GTTGATC	1280	0.0	37.96875	16
AGGGCGA	1975	0.0	37.93671	6
TAGTAGG	625	0.0	37.8	2
>>END_MODULE