FastQCFastQC Report
Thu 26 May 2016
SRR1545479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545479_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences896943
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51190.5707163108469546No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21160.23591242698811407No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10650.11873664212776064No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10150.1131621518870207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG351.2115197E-745.0000041
TAAACGC351.2115197E-745.00000427
TTATACG453.8380676E-1045.0000041
TTAAACG453.8380676E-1045.0000041
GTACGAG453.8380676E-1045.0000041
AATCCGG406.8121153E-945.02
TCGTTAG800.045.01
GTCGAAA207.0324575E-445.010
ATTCGGT253.8901184E-545.014
ACGGGTC207.0324575E-445.05
GACCCGT207.0324575E-445.032
GGACCTA406.8121153E-945.08
ACGTACG207.0324575E-445.01
CAATCTA207.0324575E-445.018
CCGTTAT253.8901184E-545.023
ACGACTG207.0324575E-445.01
ATCGAAG253.8901184E-545.01
ACGCGTA207.0324575E-445.028
ATAGACG302.1649666E-644.9999961
CTAAGCG302.1649666E-644.9999961