##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545479_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	896943
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.918506527170624	31.0	31.0	33.0	28.0	34.0
2	31.198196540917316	31.0	31.0	34.0	28.0	34.0
3	31.314626458983458	31.0	31.0	34.0	28.0	34.0
4	35.157454821543844	35.0	35.0	37.0	32.0	37.0
5	35.01883285782932	35.0	35.0	37.0	32.0	37.0
6	35.07274709764165	37.0	35.0	37.0	32.0	37.0
7	35.52217587962669	37.0	35.0	37.0	35.0	37.0
8	35.425291239242625	37.0	35.0	37.0	35.0	37.0
9	37.14580636673679	39.0	37.0	39.0	34.0	39.0
10	36.583845350261946	39.0	35.0	39.0	32.0	39.0
11	36.261992122130394	38.0	35.0	39.0	32.0	39.0
12	35.29441781696273	37.0	35.0	39.0	30.0	39.0
13	34.76632182870038	37.0	33.0	39.0	27.0	39.0
14	35.72539392135286	37.0	34.0	40.0	28.0	41.0
15	36.13580573124491	38.0	34.0	40.0	30.0	41.0
16	36.269473088033465	37.0	34.0	40.0	31.0	41.0
17	36.18915025815464	37.0	34.0	40.0	31.0	41.0
18	36.15201857866107	37.0	35.0	40.0	31.0	41.0
19	36.05276143523056	37.0	35.0	40.0	30.0	41.0
20	35.88094115233633	36.0	34.0	40.0	30.0	41.0
21	35.693223538173555	36.0	34.0	40.0	30.0	41.0
22	35.55703539689813	36.0	34.0	40.0	30.0	41.0
23	35.52965907532586	36.0	34.0	40.0	30.0	41.0
24	35.416088870753214	35.0	34.0	40.0	29.0	41.0
25	35.2995508075764	35.0	34.0	40.0	29.0	41.0
26	35.14263559668786	35.0	34.0	40.0	29.0	41.0
27	35.01830439615449	35.0	34.0	40.0	29.0	41.0
28	35.077787551717336	36.0	34.0	40.0	29.0	41.0
29	35.14772399137961	36.0	34.0	40.0	29.0	41.0
30	35.063257085455824	36.0	34.0	40.0	29.0	41.0
31	34.878846258903856	35.0	34.0	40.0	27.0	41.0
32	34.62025123112617	35.0	33.0	40.0	27.0	41.0
33	34.38563431567	35.0	33.0	40.0	25.0	41.0
34	34.23946672196561	35.0	33.0	40.0	24.0	41.0
35	34.064069846132924	35.0	33.0	40.0	23.0	41.0
36	33.814559007651546	35.0	33.0	40.0	23.0	41.0
37	33.76730962837103	35.0	33.0	40.0	23.0	41.0
38	33.79982005545503	35.0	33.0	40.0	23.0	41.0
39	33.763889121159316	35.0	33.0	40.0	23.0	41.0
40	33.55556261657653	35.0	32.0	40.0	23.0	41.0
41	33.544759254489975	35.0	33.0	39.0	23.0	41.0
42	33.49669822943041	35.0	32.0	39.0	23.0	41.0
43	33.41731191391203	35.0	32.0	39.0	23.0	41.0
44	33.363300677969505	35.0	32.0	39.0	23.0	41.0
45	33.3190570638268	35.0	32.0	39.0	23.0	41.0
46	33.27485804560602	35.0	32.0	39.0	23.0	41.0
47	33.20053002253209	35.0	32.0	39.0	23.0	41.0
48	33.1579331128065	35.0	32.0	39.0	23.0	41.0
49	33.18397044182295	35.0	32.0	39.0	22.0	41.0
50	33.07618098362995	35.0	32.0	39.0	22.0	41.0
51	32.89831237882452	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	4.0
14	10.0
15	29.0
16	110.0
17	288.0
18	719.0
19	1392.0
20	2265.0
21	3534.0
22	5420.0
23	7490.0
24	10300.0
25	14435.0
26	18775.0
27	21982.0
28	23870.0
29	26464.0
30	30737.0
31	36920.0
32	45854.0
33	58211.0
34	96228.0
35	127523.0
36	62949.0
37	73071.0
38	97253.0
39	131054.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.792942249396006	23.700391217725095	27.70633139452563	15.800335138353272
2	32.09568501008425	25.692714029765547	29.649375712837937	12.56222524731226
3	29.430632715791305	25.31387167300486	31.88864844254317	13.366847168660662
4	26.994691970392772	27.350344447751972	30.85725625820147	14.797707323653789
5	23.230238710821087	31.987874368828344	30.573626194752624	14.208260725597949
6	21.549975862457256	40.58273491180599	27.80611476983487	10.061174455901881
7	84.96548833091958	5.134662960745555	8.328288419665464	1.5715602886694027
8	86.29734553923717	3.9525365602942437	8.001177332338845	1.7489405681297472
9	81.05977748864755	6.153791266557629	9.700504937326006	3.0859263074688137
10	52.7045754300998	21.51028549194319	14.996270666028947	10.788868411928071
11	47.631343351807196	20.22347016477078	19.683525040052714	12.46166144336931
12	42.51061661666349	21.25296702243063	24.133529109430587	12.10288725147529
13	21.271362840225073	40.57716042156525	24.185037399255023	13.96643933895465
14	15.286478627961866	42.81442633478382	28.08829546582113	13.810799571433192
15	14.60538741034826	22.75584959133412	48.89719859567442	13.7415644026432
16	15.61135991919219	17.935922349580743	48.22848274639526	18.22423498483181
17	16.18965753676655	18.32111962521587	30.4348213877582	35.054401450259384
18	20.980374449658452	22.82017920871226	35.79491673383927	20.404529607790014
19	30.56091635700373	23.300365798049597	26.556982996689865	19.5817348482568
20	33.04769645339782	21.552540127967998	26.08772240822438	19.312041010409803
21	22.891421193988915	28.79469486912769	28.540163644735507	19.773720292147885
22	23.320545452721074	25.393363903837813	25.13058243388933	26.155508209551776
23	20.641779912435908	30.197236613697857	26.16152865901178	22.999454814854456
24	19.59355276756717	23.268033754653306	40.59098515736228	16.54742832041724
25	17.664444674856707	24.59587732999756	37.630373390505305	20.10930460464043
26	16.91634808454941	34.29392949161764	28.68153271724067	20.10818970659228
27	18.49069561833918	34.367289783185775	29.142989019369125	17.99902557910592
28	15.300637833173344	29.044543521717657	39.155442430566936	16.49937621454206
29	16.16412637146396	24.80547816304938	38.111563388085976	20.91883207740068
30	18.174510531884412	31.66098626111135	32.12801705348055	18.03648615352369
31	29.269418458029108	27.216333702364587	25.67833184494444	17.835915994661868
32	31.222831328189194	25.90465614871848	26.91787549487537	15.954637028216956
33	29.2083220449906	27.911918594604117	24.58963390092793	18.29012545947736
34	19.558321989245695	27.991187845827437	28.572830157546242	23.877660007380623
35	20.983161694778822	25.900308046330707	31.253379534708447	21.863150724182027
36	30.88000017838369	25.965195112732914	25.127014760135257	18.027789948748136
37	20.46116642863593	34.58157318803982	28.247837376511104	16.709423006813143
38	21.04659939371844	34.3056359211232	23.786126877627677	20.861637807530688
39	21.380734338748393	33.14759131851188	26.554307241374314	18.917367101365414
40	24.764672894487163	26.594778040522083	25.63819551521111	23.00235354977964
41	17.506798090848584	25.830515428516637	27.597405855221567	29.06528062541321
42	21.330563926581732	26.800476730405386	24.3814824353387	27.48747690767418
43	22.086799272640516	27.13305081816793	25.67476417119037	25.10538573800119
44	19.576940786649764	30.310510255389694	28.87151134464509	21.24103761331545
45	17.470452414478956	37.69481449768826	23.363246047965145	21.47148703986764
46	20.868438685624394	34.40430439838429	25.47185272642743	19.255404189563883
47	22.788962063364117	27.964207313062257	26.521083279539504	22.725747344034126
48	23.491013364282903	24.094730657355036	31.41704656817657	20.997209410185487
49	21.475166203426525	25.26927575107894	32.302944557234966	20.952613488259566
50	19.393763037339053	34.57309996287389	26.493656787555064	19.539480212231993
51	18.87455501631653	34.6093341494387	24.386276496945737	22.129834337299027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	691.0
1	1146.0
2	1601.0
3	5556.0
4	9511.0
5	7108.5
6	4706.0
7	4908.0
8	5110.0
9	5322.0
10	5534.0
11	5675.0
12	5816.0
13	5692.5
14	5569.0
15	5455.0
16	5341.0
17	4864.5
18	4388.0
19	4142.0
20	3896.0
21	3862.5
22	3829.0
23	4127.5
24	4426.0
25	4907.5
26	5852.5
27	6316.0
28	8477.5
29	10639.0
30	12929.0
31	15219.0
32	17494.0
33	19769.0
34	21011.0
35	22253.0
36	22774.5
37	23296.0
38	28871.0
39	34446.0
40	46830.5
41	59215.0
42	73415.5
43	87616.0
44	90348.5
45	93081.0
46	86206.0
47	79331.0
48	73997.0
49	68663.0
50	64168.0
51	59673.0
52	56979.0
53	54285.0
54	48570.5
55	42856.0
56	42012.5
57	41169.0
58	38376.0
59	35583.0
60	32418.0
61	29253.0
62	23834.0
63	18415.0
64	15393.0
65	12371.0
66	9894.5
67	7418.0
68	5743.5
69	4069.0
70	3314.0
71	2559.0
72	2094.5
73	1630.0
74	1304.0
75	709.5
76	441.0
77	383.0
78	325.0
79	239.5
80	154.0
81	112.0
82	70.0
83	45.5
84	21.0
85	16.0
86	11.0
87	10.0
88	9.0
89	4.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	896943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.263207308633657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45628384348856	15.498460749593928
2	5.25283939409307	2.0237306841474485
3	1.7720272466653253	1.024047846271844
4	1.0624759854029566	0.8186678066904792
5	0.7226461018300062	0.6960240835163698
6	0.5938559531119708	0.6863742201757274
7	0.46480271886217484	0.6267513791741048
8	0.4303809835482379	0.6632414486226687
9	0.40515499643885117	0.7024126219677287
>10	5.780848265467053	27.522743932304472
>50	2.440353621841193	33.27780811275523
>100	0.6137035299297241	15.139940831680645
>500	0.002313679660432513	0.28189823522510493
>1k	0.001735259745324385	0.4675276660096997
>5k	5.784199151081282E-4	0.5703703818645502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5119	0.5707163108469546	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2116	0.23591242698811407	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1065	0.11873664212776064	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1015	0.1131621518870207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009699613018887488	0.0
2	0.0	0.0	0.0	0.040136329733327536	0.0
3	0.0	0.0	0.0	0.05607937182184375	0.0
4	0.0	0.0	0.0	0.08517821087850622	0.0
5	0.0	0.0	0.0	0.13546011284998044	0.0
6	0.0	0.0	0.0	0.19689099530293452	0.0
7	0.0	0.0	0.0	0.23000346733292973	0.0
8	0.0	0.0	0.0	0.31585061704032474	0.0
9	0.0	0.0	0.0	0.35130437497143074	0.0
10	0.0	0.0	0.0	0.4069377875740153	0.0
11	0.0	0.0	0.0	0.47048697631845054	0.0
12	0.0	0.0	0.0	0.5276812461884423	0.0
13	0.0	0.0	0.0	0.5519860236380684	0.0
14	0.0	0.0	0.0	0.5625775550954742	0.0
15	0.0	0.0	0.0	0.5759563316732501	0.0
16	0.0	0.0	0.0	0.6094032731176897	0.0
17	0.0	0.0	0.0	0.6509889703136097	0.0
18	0.0	0.0	0.0	0.7070683421354534	0.0
19	0.0	0.0	0.0	0.7291433234887835	0.0
20	0.0	0.0	0.0	0.7552319378154465	0.0
21	0.0	0.0	0.0	0.7928040020380336	0.0
22	0.0	0.0	0.0	0.829707127431732	0.0
23	0.0	0.0	0.0	0.8737456003335775	0.0
24	0.0	0.0	0.0	0.9098682970935723	0.0
25	0.0	0.0	0.0	0.9359569114202352	0.0
26	0.0	0.0	0.0	0.9666166077443048	0.0
27	0.0	0.0	0.0	0.9927052220709677	0.0
28	0.0	0.0	0.0	1.0235878980046669	0.0
29	0.0	0.0	0.0	1.0575922884731805	0.0
30	0.0	0.0	0.0	1.0998469244979894	0.0
31	0.0	0.0	0.0	1.1474530711539084	0.0
32	0.0	0.0	0.0	1.1891502581546431	0.0
33	0.0	0.0	0.0	1.2304014859361185	0.0
34	0.0	0.0	0.0	1.2747744282524085	0.0
35	0.0	0.0	0.0	1.3384351068016584	0.0
36	0.0	0.0	0.0	1.389162967992392	0.0
37	0.0	0.0	0.0	1.440113808792755	0.0
38	0.0	0.0	0.0	1.4940748743231176	0.0
39	0.0	0.0	0.0	1.5473670010245912	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	35	1.2115197E-7	45.000004	1
TAAACGC	35	1.2115197E-7	45.000004	27
TTATACG	45	3.8380676E-10	45.000004	1
TTAAACG	45	3.8380676E-10	45.000004	1
GTACGAG	45	3.8380676E-10	45.000004	1
AATCCGG	40	6.8121153E-9	45.0	2
TCGTTAG	80	0.0	45.0	1
GTCGAAA	20	7.0324575E-4	45.0	10
ATTCGGT	25	3.8901184E-5	45.0	14
ACGGGTC	20	7.0324575E-4	45.0	5
GACCCGT	20	7.0324575E-4	45.0	32
GGACCTA	40	6.8121153E-9	45.0	8
ACGTACG	20	7.0324575E-4	45.0	1
CAATCTA	20	7.0324575E-4	45.0	18
CCGTTAT	25	3.8901184E-5	45.0	23
ACGACTG	20	7.0324575E-4	45.0	1
ATCGAAG	25	3.8901184E-5	45.0	1
ACGCGTA	20	7.0324575E-4	45.0	28
ATAGACG	30	2.1649666E-6	44.999996	1
CTAAGCG	30	2.1649666E-6	44.999996	1
>>END_MODULE