FastQCFastQC Report
Thu 26 May 2016
SRR1545478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545478_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2266462
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132920.5864647190202175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58800.259435190177466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC453.8562575E-1045.029
GTACGAT207.03474E-445.035
AATCCGT302.16644E-644.99999610
CACGACG2450.043.16326526
CGACGGT2350.043.08510628
CGTTTTT118600.042.7424131
ATCGCCA2250.042.024
CGGTCTA2500.041.431
ACGGGTA2600.039.8076935
TCGATTG850.039.7058831
GACGGTC2550.039.70588329
TAACGGG7600.039.6710553
TACGCGA403.460209E-739.37512
CCGTAAA403.460209E-739.37518
GTAGGGT10900.038.8073434
TCACGAC2800.038.57142625
CGCGAAA356.250979E-638.57142617
TCGCTTA356.250979E-638.57142635
ACTCGAC356.250979E-638.57142632
TAATGCG700.038.5714261
Produced by FastQC (version 0.11.5)