FastQCFastQC Report
Thu 26 May 2016
SRR1545477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545477_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1761973
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95720.5432546355704656No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38440.21816452351994045No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26200.14869694371026115No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25440.14438359725149022No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24660.13995674167538322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC453.8562575E-1045.0000049
TCTAACG453.8562575E-1045.0000041
TAATACG253.891655E-545.01
GATACGT502.1827873E-1145.09
CGTGCAC551.8189894E-1245.017
CTATACG207.034313E-445.01
TGACGTA207.034313E-445.014
ATCGTTC253.891655E-545.010
CCCGTTC207.034313E-445.018
CCCGTGC207.034313E-445.017
TGCGCGA207.034313E-445.015
TCTACGA207.034313E-445.029
CATACGT207.034313E-445.024
CGTAACG253.891655E-545.032
TACTACC207.034313E-445.039
CCACGAA253.891655E-545.010
ATACCCG207.034313E-445.043
TACACCG253.891655E-545.045
AATACGG1250.043.1999972
ACGTAAG1100.042.9545441