FastQCFastQC Report
Thu 26 May 2016
SRR1545476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545476_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1158917
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99290.8567481536641537No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24960.21537349094024852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAT302.1655178E-645.00000416
TATCGCG453.8562575E-1045.0000041
TAATACG253.8908256E-545.01
TAGACGT253.8908256E-545.036
ATTACGT207.0333126E-445.017
CTTATCG253.8908256E-545.01
ACGTAAG406.8157533E-945.01
CGTTTTT69750.042.838711
AATACGG900.042.5000042
TATACGG1450.040.3448262
CTATGCG750.039.01
TCGTTGA1450.038.79310224
TACGGGA2850.038.684214
AACGTCG356.248334E-638.57142612
TTATACG356.248334E-638.5714261
TATCGAC356.248334E-638.57142613
GTACGAG1000.038.251
AGGGCGA9950.038.216086
TCGATAG659.094947E-1238.0769271
GCCCGAA659.094947E-1238.07692725
Produced by FastQC (version 0.11.5)