##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545476_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1158917 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06020362113939 31.0 31.0 33.0 30.0 34.0 2 31.35874527684036 31.0 31.0 34.0 30.0 34.0 3 31.44554269201332 31.0 31.0 34.0 30.0 34.0 4 35.28689457484876 37.0 35.0 37.0 33.0 37.0 5 35.19216561669214 37.0 35.0 37.0 32.0 37.0 6 35.24000683396654 37.0 35.0 37.0 32.0 37.0 7 35.65808940588498 37.0 35.0 37.0 35.0 37.0 8 35.5747987129363 37.0 35.0 37.0 35.0 37.0 9 37.27710957730364 39.0 37.0 39.0 34.0 39.0 10 36.71887719310356 39.0 37.0 39.0 32.0 39.0 11 36.4636751380815 38.0 35.0 39.0 32.0 39.0 12 35.90215175029791 37.0 35.0 39.0 31.0 39.0 13 35.52773408276865 37.0 35.0 39.0 30.0 39.0 14 36.565559052115034 38.0 35.0 40.0 31.0 41.0 15 36.83514436322877 38.0 35.0 40.0 31.0 41.0 16 36.892194177840175 38.0 35.0 40.0 32.0 41.0 17 36.80859457579792 38.0 35.0 40.0 31.0 41.0 18 36.73460308201536 38.0 35.0 40.0 31.0 41.0 19 36.65515649524513 38.0 35.0 40.0 31.0 41.0 20 36.51737699938822 38.0 35.0 40.0 31.0 41.0 21 36.353630156430526 38.0 35.0 40.0 31.0 41.0 22 36.25553253597971 38.0 35.0 40.0 30.0 41.0 23 36.19542124241857 38.0 35.0 40.0 30.0 41.0 24 36.09262613284644 37.0 34.0 40.0 30.0 41.0 25 35.96329245321279 37.0 34.0 40.0 30.0 41.0 26 35.85619591394379 37.0 34.0 40.0 30.0 41.0 27 35.76818357138605 37.0 34.0 40.0 30.0 41.0 28 35.77808764562087 37.0 34.0 40.0 30.0 41.0 29 35.78944307487076 37.0 34.0 40.0 30.0 41.0 30 35.67009975692824 37.0 34.0 40.0 30.0 41.0 31 35.52157919851033 37.0 34.0 40.0 29.0 41.0 32 35.29425403199711 37.0 34.0 40.0 28.0 41.0 33 35.038384974937806 37.0 34.0 40.0 27.0 41.0 34 34.81954963125055 37.0 34.0 40.0 25.0 41.0 35 34.6181736914723 37.0 33.0 40.0 24.0 41.0 36 34.45985950676364 37.0 33.0 40.0 23.0 41.0 37 34.39678078758013 37.0 33.0 40.0 23.0 41.0 38 34.34183207252978 37.0 33.0 40.0 23.0 41.0 39 34.286026523038316 36.0 33.0 40.0 23.0 41.0 40 34.13371104229207 36.0 33.0 40.0 23.0 41.0 41 34.04496611923028 36.0 33.0 40.0 23.0 41.0 42 34.003988206230474 36.0 33.0 40.0 23.0 41.0 43 33.89775885589736 36.0 33.0 40.0 23.0 41.0 44 33.8291611910085 36.0 33.0 40.0 23.0 41.0 45 33.76679434333951 35.0 33.0 40.0 23.0 41.0 46 33.72193004330767 35.0 33.0 40.0 23.0 41.0 47 33.641226248299056 35.0 33.0 40.0 23.0 41.0 48 33.57766604510935 35.0 33.0 40.0 23.0 41.0 49 33.579267540298396 35.0 33.0 40.0 22.0 41.0 50 33.49028877823002 35.0 32.0 39.0 22.0 41.0 51 33.32494389158154 35.0 32.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 6.0 12 7.0 13 7.0 14 20.0 15 47.0 16 113.0 17 292.0 18 698.0 19 1328.0 20 2421.0 21 3781.0 22 5611.0 23 8325.0 24 11783.0 25 16835.0 26 22079.0 27 25563.0 28 26880.0 29 29583.0 30 34345.0 31 42278.0 32 52546.0 33 66913.0 34 106633.0 35 137826.0 36 93163.0 37 112655.0 38 152850.0 39 204278.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.56366331669999 22.301338232159853 27.242330555164866 16.892667895975293 2 33.487644067694234 24.3778458681683 28.962039559347218 13.17247050479025 3 29.066533668934014 24.70047466729714 33.139215319129846 13.093776344639005 4 26.060796415964216 26.88837940939688 32.625200941913874 14.425623232725036 5 22.191494300282073 31.633326631674226 32.18194227886898 13.99323678917472 6 21.56073299468383 38.86792583075406 29.730860795035362 9.840480379526747 7 83.79763175447422 4.593857886285213 9.79578347716014 1.812726882080425 8 84.95845690416138 3.746342490445821 9.414306632830478 1.8808939725623146 9 79.37729794282076 5.682287860131485 11.424890652220995 3.515523544826765 10 44.858605059723864 23.644316202109383 18.92214886829687 12.574929869869885 11 37.80797071748883 23.91094444209551 24.51374861185055 13.767336228565116 12 34.32609928062148 22.108917204597052 28.67867155283769 14.886311961943779 13 22.17389165919561 33.077778650239836 28.65899801279988 16.08933167776467 14 17.517647942001023 34.98145251126699 31.42036918951055 16.08053035722144 15 16.78446342576733 23.52437663784378 43.46549407766044 16.22566585872845 16 18.94967456685854 20.00574674458999 41.85390325622974 19.190675432321726 17 18.83939919769923 20.801144516820443 32.2546826045351 28.10477368094523 18 22.072072460754306 22.94443864400988 35.333419045539934 19.650069849695882 19 27.55607174629417 23.49132854207851 29.32634519987195 19.626254511755373 20 29.078009900622735 22.383742752932264 29.545515338889672 18.99273200755533 21 23.564759167395078 25.930416069485563 30.743444094788497 19.761380668330865 22 23.4256637878295 24.026224483720576 29.153511424890656 23.394600303559272 23 20.844805969711377 27.127309375908716 29.32021879047421 22.7076658639057 24 19.83783135461815 24.08895546445518 38.06036152718443 18.012851653742242 25 19.715475741575972 24.85492921408522 35.3970991882939 20.03249585604491 26 18.4750935571745 29.777542308897015 30.460938962842032 21.286425171086453 27 18.62523373114727 30.868733481345085 31.125093514030773 19.380939273476873 28 17.68979141733187 27.720880787838993 36.03088055486286 18.558447239966277 29 18.179213869500575 25.20758604800861 35.566481465022946 21.04671861746786 30 19.44341139184256 28.287099076120203 33.520347013634286 18.749142518402955 31 25.417091991920042 27.17839155004198 29.192427067684747 18.212089390353235 32 27.25441079904773 25.629013984608047 29.274055001350398 17.842520214993826 33 25.618832064763915 26.983468186246295 28.28356129041165 19.11413845857814 34 20.479810029536196 27.380217910342157 30.494936220626673 21.645035839494977 35 21.17804812596588 26.54883826883202 31.084279547197944 21.188834058004154 36 26.76119169880155 27.3459617901886 27.102027151211 18.790819359798846 37 21.422500489681315 30.915673857575648 29.731809957054733 17.930015695688304 38 21.48445488330916 30.59753200617473 27.080455287134452 20.83755782338166 39 21.06095604775838 30.5859694870297 28.729149714776813 19.623924750435105 40 23.688150229912928 27.045077430048913 28.076989120014634 21.18978322002352 41 19.120092293063266 26.691902871387686 29.656394720243124 24.531610115305927 42 21.824427461155544 26.49335543442714 27.25993319625133 24.422283908165987 43 21.47919134847448 26.68310155084445 28.923900503659883 22.913806597021185 44 20.512685550388852 28.932184099465275 29.568985527004955 20.986144823140915 45 18.12183271105696 33.79750232328976 27.22723025031128 20.853434715341994 46 21.354074536830506 31.309662383069707 27.398338276166456 19.937924803933328 47 21.300921463745894 28.96816596874496 29.004061550568334 20.726851016940817 48 22.155512431002393 26.96992105560623 30.823432566784337 20.051133946607045 49 21.548652750800965 26.544955333298244 31.82134699896541 20.08504491693538 50 20.148552484776737 30.97659280172782 29.470617826815896 19.404236886679545 51 19.439873606134004 31.34314191611651 27.38737976921557 21.829604708533914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 918.0 1 1684.0 2 2450.0 3 9915.0 4 17380.0 5 12504.0 6 7628.0 7 7750.5 8 7873.0 9 8239.0 10 8605.0 11 8524.0 12 8443.0 13 8248.5 14 8054.0 15 7521.0 16 6988.0 17 6676.5 18 6365.0 19 6163.0 20 5961.0 21 6299.0 22 6637.0 23 7244.5 24 7852.0 25 8198.0 26 9929.0 27 11314.0 28 14888.5 29 18463.0 30 20806.0 31 23149.0 32 27520.5 33 31892.0 34 33472.0 35 35052.0 36 37366.0 37 39680.0 38 45395.5 39 51111.0 40 60537.5 41 69964.0 42 80751.0 43 91538.0 44 92502.5 45 93467.0 46 93384.5 47 93302.0 48 86842.0 49 80382.0 50 75968.0 51 71554.0 52 67553.5 53 63553.0 54 61879.5 55 60206.0 56 58303.5 57 56401.0 58 51770.0 59 47139.0 60 42871.5 61 38604.0 62 33734.5 63 28865.0 64 24169.5 65 19474.0 66 15654.5 67 11835.0 68 9749.0 69 7663.0 70 5947.5 71 4232.0 72 3527.5 73 2823.0 74 2246.5 75 1307.0 76 944.0 77 755.0 78 566.0 79 373.0 80 180.0 81 133.5 82 87.0 83 75.5 84 64.0 85 41.0 86 18.0 87 15.5 88 13.0 89 12.0 90 11.0 91 6.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1158917.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.11988006490879 #Duplication Level Percentage of deduplicated Percentage of total 1 77.46339631105693 15.585542431989632 2 5.52497729286033 2.2232376098738857 3 2.3625296133811458 1.4260143741307199 4 1.437447931760528 1.1568511994629207 5 1.060849102000078 1.0672078349603882 6 0.8301809932047086 1.0021885209267358 7 0.6954534512583631 0.9794708021031612 8 0.5875894432929566 0.9457783301168649 9 0.5023273442756113 0.9096089328134503 >10 6.936730250871063 33.95952489669855 >50 2.3119486729527705 32.20520201293926 >100 0.284845866209845 7.339057483273096 >500 8.618634378512707E-4 0.12303147896846303 >1k 4.3093171892563534E-4 0.2164105507436813 >5k 4.3093171892563534E-4 0.8608735409992032 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9929 0.8567481536641537 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2496 0.21537349094024852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007679583611250849 0.0 2 0.0 0.0 0.0 0.029596597513022935 0.0 3 0.0 0.0 0.0 0.04081396683282754 0.0 4 0.0 0.0 0.0 0.057294870987309704 0.0 5 0.0 0.0 0.0 0.09008410438366164 0.0 6 0.0 0.0 0.0 0.1325375328863068 0.0 7 0.0 0.0 0.0 0.15523113389483456 0.0 8 0.0 0.0 0.0 0.22270792472627463 0.0 9 0.0 0.0 0.0 0.2508376354820923 0.0 10 0.0 0.0 0.0 0.3026101092658059 0.0 11 0.0 0.0 0.0 0.3515350969914153 0.0 12 0.0 0.0 0.0 0.39053702724181283 0.0 13 0.0 0.0 0.0 0.40727679376521353 0.0 14 0.0 0.0 0.0 0.41616440176475106 0.0 15 0.0 0.0 0.0 0.4291938076669856 0.0 16 0.0 0.0 0.0 0.4527502832385753 0.0 17 0.0 0.0 0.0 0.47993083197502495 0.0 18 0.0 0.0 0.0 0.5230745601281196 0.0 19 0.0 0.0 0.0 0.544214986923136 0.0 20 0.0 0.0 0.0 0.5646651140677028 0.0 21 0.0 0.0 0.0 0.592967399736133 0.0 22 0.0 0.0 0.0 0.6228228596180745 0.0 23 0.0 0.0 0.0 0.6549217933639769 0.0 24 0.0 0.0 0.0 0.6831377915761008 0.0 25 0.0 0.0 0.0 0.7082474413612019 0.0 26 0.0 0.0 0.0 0.7324942165832411 0.0 27 0.0 0.0 0.0 0.7600199151449155 0.0 28 0.0 0.0 0.0 0.7870278889687528 0.0 29 0.0 0.0 0.0 0.8171422112196128 0.0 30 0.0 0.0 0.0 0.8565755787515413 0.0 31 0.0 0.0 0.0 0.8877253504780757 0.0 32 0.0 0.0 0.0 0.9235346448451442 0.0 33 0.0 0.0 0.0 0.9613285507072551 0.0 34 0.0 0.0 0.0 0.9998127562198156 0.0 35 0.0 0.0 0.0 1.0463216951688517 0.0 36 0.0 0.0 0.0 1.0862727874386173 0.0 37 0.0 0.0 0.0 1.1301931026984675 0.0 38 8.628745630618931E-5 0.0 0.0 1.1760980294533603 0.0 39 8.628745630618931E-5 0.0 0.0 1.2371032610618362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGAGAT 30 2.1655178E-6 45.000004 16 TATCGCG 45 3.8562575E-10 45.000004 1 TAATACG 25 3.8908256E-5 45.0 1 TAGACGT 25 3.8908256E-5 45.0 36 ATTACGT 20 7.0333126E-4 45.0 17 CTTATCG 25 3.8908256E-5 45.0 1 ACGTAAG 40 6.8157533E-9 45.0 1 CGTTTTT 6975 0.0 42.83871 1 AATACGG 90 0.0 42.500004 2 TATACGG 145 0.0 40.344826 2 CTATGCG 75 0.0 39.0 1 TCGTTGA 145 0.0 38.793102 24 TACGGGA 285 0.0 38.68421 4 AACGTCG 35 6.248334E-6 38.571426 12 TTATACG 35 6.248334E-6 38.571426 1 TATCGAC 35 6.248334E-6 38.571426 13 GTACGAG 100 0.0 38.25 1 AGGGCGA 995 0.0 38.21608 6 TCGATAG 65 9.094947E-12 38.076927 1 GCCCGAA 65 9.094947E-12 38.076927 25 >>END_MODULE