##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545475_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1016912
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93985418600626	31.0	31.0	33.0	28.0	34.0
2	31.222449926837328	31.0	31.0	34.0	28.0	34.0
3	31.287712211086113	31.0	31.0	34.0	28.0	34.0
4	35.11334314080274	35.0	35.0	37.0	32.0	37.0
5	34.966308785814306	35.0	35.0	37.0	32.0	37.0
6	35.024070912724014	37.0	35.0	37.0	32.0	37.0
7	35.57798118224586	37.0	35.0	37.0	35.0	37.0
8	35.5218740657992	37.0	35.0	37.0	35.0	37.0
9	37.226951791305446	39.0	37.0	39.0	34.0	39.0
10	36.603546816243686	39.0	35.0	39.0	32.0	39.0
11	36.24854363012729	38.0	35.0	39.0	32.0	39.0
12	35.168037155624084	37.0	35.0	39.0	30.0	39.0
13	34.56001895935932	37.0	33.0	39.0	27.0	39.0
14	35.4787533237881	37.0	33.0	40.0	27.0	41.0
15	35.945081776987585	37.0	34.0	40.0	30.0	41.0
16	36.12855979671791	37.0	34.0	40.0	31.0	41.0
17	36.05771689192378	37.0	34.0	40.0	31.0	41.0
18	35.981023923407335	36.0	34.0	40.0	30.0	41.0
19	35.83950331985462	36.0	34.0	40.0	30.0	41.0
20	35.65520615353147	36.0	34.0	40.0	30.0	41.0
21	35.452517031955566	35.0	34.0	40.0	30.0	41.0
22	35.323975919253584	35.0	34.0	40.0	29.0	41.0
23	35.32983384992998	35.0	34.0	40.0	29.0	41.0
24	35.233998615416084	35.0	34.0	40.0	29.0	41.0
25	35.112056893811854	35.0	34.0	40.0	29.0	41.0
26	34.92224892616077	35.0	34.0	40.0	29.0	41.0
27	34.78384560315937	35.0	34.0	40.0	27.0	41.0
28	34.86425570747518	35.0	34.0	40.0	28.0	41.0
29	34.93875084569756	36.0	34.0	40.0	29.0	41.0
30	34.828554486523906	36.0	34.0	40.0	28.0	41.0
31	34.598665371241566	35.0	33.0	40.0	27.0	41.0
32	34.323372130528504	35.0	33.0	40.0	25.0	41.0
33	34.048022837767675	35.0	33.0	40.0	24.0	41.0
34	33.84625414981827	35.0	33.0	40.0	23.0	41.0
35	33.63212057680507	35.0	33.0	40.0	23.0	41.0
36	33.39163565775603	35.0	33.0	39.0	21.0	41.0
37	33.324014270654686	35.0	32.0	39.0	22.0	41.0
38	33.341137679563225	35.0	32.0	39.0	23.0	41.0
39	33.300385874097266	35.0	32.0	39.0	22.0	41.0
40	33.08045533930173	35.0	32.0	39.0	21.0	41.0
41	33.076574964205356	35.0	32.0	39.0	21.0	41.0
42	33.04968178170776	35.0	32.0	39.0	21.0	41.0
43	32.90392875686392	35.0	32.0	39.0	20.0	41.0
44	32.86324775398461	35.0	32.0	39.0	20.0	41.0
45	32.79539527510738	35.0	32.0	39.0	20.0	41.0
46	32.75791120568938	35.0	32.0	39.0	20.0	40.0
47	32.71178135374546	35.0	32.0	39.0	20.0	40.0
48	32.70795211385056	35.0	32.0	38.0	20.0	40.0
49	32.734850213194456	35.0	32.0	38.0	20.0	40.0
50	32.61953935050427	35.0	32.0	38.0	20.0	40.0
51	32.41232279685951	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	4.0
14	11.0
15	62.0
16	177.0
17	424.0
18	1036.0
19	1856.0
20	3109.0
21	4833.0
22	7100.0
23	9752.0
24	13622.0
25	18544.0
26	23994.0
27	26817.0
28	28558.0
29	30963.0
30	35660.0
31	43002.0
32	52559.0
33	67242.0
34	111668.0
35	152412.0
36	66638.0
37	77556.0
38	102177.0
39	137077.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41942272291015	23.530649653067325	26.378782038170463	17.67114558585207
2	32.72603725789448	24.973449030004563	29.250711959343583	13.049801752757368
3	28.073422282360717	25.12921472064446	33.15203282093239	13.645330176062432
4	26.10009519014428	27.057896848498196	31.77659423824284	15.065413723114684
5	22.48168966439574	31.447165536447596	31.83136790597417	14.239776893182498
6	21.758913258964395	38.85321443743411	29.302633856223547	10.085238447377943
7	84.06125603788726	5.1686871627043445	9.222233585600327	1.5478232138080776
8	85.711054643863	3.8472355523388457	8.791419513192881	1.6502902906052834
9	81.65613150400428	5.055993045612599	10.652052488317572	2.6358229620655473
10	53.51603678587724	20.88656638922542	15.769801123401042	9.827595701496294
11	50.018192331293164	20.027396667558257	19.60346617996444	10.350944821184134
12	44.16842361974291	22.161897981339585	22.811806724672344	10.857871674245166
13	20.276582437811726	44.6269687052567	22.732547162389665	12.363901694541907
14	13.71544440423557	45.31778560976761	28.403047658007775	12.56372232798905
15	12.953038217662884	22.330250798495836	52.92955535975581	11.787155624085468
16	14.314512956873356	17.450379187186304	50.78040184401404	17.4547060119263
17	14.719857765470366	17.586674166496216	30.728322608052615	36.9651454599808
18	21.055509227937126	21.799133061661184	37.547988419843605	19.59736929055808
19	30.50549113394276	22.503127114243906	27.58075428355649	19.41062746825684
20	32.713056783674496	21.459969004200953	26.21524773038375	19.6117264817408
21	21.42102758154098	28.314249413911924	29.960802901332663	20.303920103214438
22	21.847416492282516	25.160485863083533	26.428638859606334	26.56345878502761
23	18.630520635020535	31.250590021555453	26.792387148543828	23.326502194880185
24	18.407984171688405	22.69065563195242	43.36058577340026	15.54077442295892
25	15.925665150966847	23.868732004342558	40.26051418411819	19.9450886605724
26	15.573815630064352	35.788052456849755	29.329479836996715	19.30865207608918
27	16.412039586512893	36.64791053699828	30.1769474644807	16.76310241200812
28	13.853509448211842	29.503437858929782	40.84473386094372	15.79831883191466
29	14.675212801107667	24.883274068945983	40.74698695659015	19.6945261733562
30	17.562384945796687	31.305462026212695	33.59268058593074	17.539472442059882
31	29.16122535676637	26.717946095630694	26.397269380241354	17.72355916736158
32	31.007796151486065	26.539267901253993	27.64486995924918	14.808065988010762
33	29.281294743301288	26.403366269647716	25.753064178611325	18.562274808439668
34	19.658731532325312	27.513885173938355	29.244025048381765	23.583358245354564
35	20.505412464402035	25.78708875497585	32.663888320719984	21.043610459902133
36	31.138387589093252	25.489324543323317	25.929972308321663	17.442315559261765
37	19.985898484824645	35.12014805607565	28.552126437685853	16.34182702141385
38	20.73070236165961	34.781377346319054	23.871682112119828	20.616238179901504
39	20.6118130182356	33.04435388706201	26.927207073965103	19.41662602073729
40	24.53535802507985	25.76496294664632	26.522550623849455	23.177128404424373
41	16.035114149503595	25.063132306433594	28.434122126595025	30.467631417467782
42	22.185105495854117	25.912468335509857	24.729966801453813	27.172459367182217
43	21.891471435089763	26.972147049105526	26.308372799219597	24.828008716585114
44	19.08847569897887	32.14909451358623	29.048236228896897	19.714193558538003
45	16.63683779914093	39.398984376229215	23.318536903881554	20.645640920748303
46	20.359480466353038	34.91993407492487	26.051221737967495	18.669363720754596
47	22.079294963576	28.006553172742578	26.907736362635116	23.006415501046305
48	23.614334377015908	23.887907704894822	31.95428906336045	20.54346885472883
49	20.781935900058215	25.0555603631386	33.07769010494517	21.08481363185802
50	18.69542300612049	36.06142911087685	26.106880438032004	19.136267444970656
51	17.880996585741933	36.070279434208665	23.439392985823748	22.609330994225658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	976.0
1	1516.5
2	2057.0
3	6548.0
4	11039.0
5	8438.5
6	5838.0
7	6243.5
8	6649.0
9	7307.5
10	7966.0
11	8039.0
12	8112.0
13	7897.5
14	7683.0
15	7393.5
16	7104.0
17	6674.0
18	6244.0
19	5815.0
20	5386.0
21	5363.5
22	5341.0
23	5542.0
24	5743.0
25	6023.5
26	6914.5
27	7525.0
28	8956.0
29	10387.0
30	11779.0
31	13171.0
32	17173.0
33	21175.0
34	23092.5
35	25010.0
36	24955.0
37	24900.0
38	32350.5
39	39801.0
40	55420.0
41	71039.0
42	90575.0
43	110111.0
44	109953.0
45	109795.0
46	104406.0
47	99017.0
48	90095.5
49	81174.0
50	76892.5
51	72611.0
52	67022.0
53	61433.0
54	53367.0
55	45301.0
56	41770.5
57	38240.0
58	35333.5
59	32427.0
60	28703.0
61	24979.0
62	21109.0
63	17239.0
64	13921.5
65	10604.0
66	8131.0
67	5658.0
68	4448.0
69	3238.0
70	2763.5
71	2289.0
72	1823.0
73	1357.0
74	1116.0
75	684.5
76	494.0
77	399.5
78	305.0
79	224.0
80	143.0
81	105.0
82	67.0
83	72.0
84	77.0
85	47.5
86	18.0
87	11.5
88	5.0
89	3.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1016912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.374970830319622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01152754588263	16.50607510666993
2	6.538858828733267	2.6645811579803653
3	2.3940096563471824	1.4633363074673207
4	1.2420708911463192	1.012286327051814
5	0.7904756453592381	0.8052959108136278
6	0.5004132272202055	0.6117542944630677
7	0.3793379170668831	0.5410299296550362
8	0.31202511162028534	0.508600203807243
9	0.2614879702572926	0.47950287898246396
>10	3.7387805668113714	18.838073841207965
>50	1.8073285556052932	27.097502647513753
>100	1.0207745128919616	28.206973239582332
>500	0.0019397140387495708	0.2670673265983069
>1k	4.849285096873927E-4	0.4132973093454376
>5k	4.849285096873927E-4	0.5846235188613327
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5917	0.5818595906037101	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4183	0.411343361077458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014750538886353982	0.0
2	0.0	0.0	0.0	0.04975848450996743	0.0
3	0.0	0.0	0.0	0.06863917428450053	0.0
4	0.0	0.0	0.0	0.10463048916720424	0.0
5	0.0	0.0	0.0	0.16235426467580283	0.0
6	0.0	0.0	0.0	0.2396470884402977	0.0
7	0.0	0.0	0.0	0.2834070204698145	0.0
8	0.0	0.0	0.0	0.4046564501156442	0.0
9	0.0	0.0	0.0	0.4641502902906053	0.0
10	0.0	0.0	0.0	0.563273911606904	0.0
11	0.0	0.0	0.0	0.6570873389241154	0.0
12	0.0	0.0	0.0	0.7398870305395157	0.0
13	9.833692590902655E-5	0.0	0.0	0.774403291533584	0.0
14	9.833692590902655E-5	0.0	0.0	0.7904322104567554	0.0
15	9.833692590902655E-5	0.0	0.0	0.8122630080085592	0.0
16	9.833692590902655E-5	0.0	0.0	0.8631031987035259	0.0
17	9.833692590902655E-5	0.0	0.0	0.9136483786207656	0.0
18	9.833692590902655E-5	0.0	0.0	0.9927112670516229	0.0
19	9.833692590902655E-5	0.0	0.0	1.0230973771575123	0.0
20	9.833692590902655E-5	0.0	0.0	1.0590886920402158	0.0
21	9.833692590902655E-5	0.0	0.0	1.1062904164765486	0.0
22	9.833692590902655E-5	0.0	0.0	1.1498536746542474	0.0
23	9.833692590902655E-5	0.0	0.0	1.2011855499787591	0.0
24	9.833692590902655E-5	0.0	0.0	1.2388485926019164	0.0
25	9.833692590902655E-5	0.0	0.0	1.2698247242632597	0.0
26	9.833692590902655E-5	0.0	0.0	1.3056193652941455	0.0
27	9.833692590902655E-5	0.0	0.0	1.3387589093254875	0.0
28	1.966738518180531E-4	0.0	0.0	1.374356876504555	0.0
29	1.966738518180531E-4	0.0	0.0	1.411528234498167	0.0
30	1.966738518180531E-4	0.0	0.0	1.4653185329704046	0.0
31	1.966738518180531E-4	0.0	0.0	1.5153720282580991	0.0
32	1.966738518180531E-4	0.0	0.0	1.5604103403244332	0.0
33	1.966738518180531E-4	0.0	0.0	1.6046619569834952	0.0
34	1.966738518180531E-4	0.0	0.0	1.64861856286483	0.0
35	1.966738518180531E-4	0.0	0.0	1.7130292493352424	0.0
36	2.9501077772707963E-4	0.0	0.0	1.7608209953270293	0.0
37	2.9501077772707963E-4	0.0	0.0	1.8147096307251758	0.0
38	2.9501077772707963E-4	0.0	0.0	1.8623047028651447	0.0
39	2.9501077772707963E-4	0.0	0.0	1.9147182843746557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	35	1.211738E-7	45.000004	37
TTAGCCG	35	1.211738E-7	45.000004	22
CATAGCG	35	1.211738E-7	45.000004	1
CGTCCTA	35	1.211738E-7	45.000004	42
TATCGTG	35	1.211738E-7	45.000004	1
AGCGTTG	25	3.890487E-5	45.0	1
CTAGACG	55	1.8189894E-12	45.0	1
TCGCATG	20	7.032904E-4	45.0	1
CGTATCT	20	7.032904E-4	45.0	44
ACGCATT	20	7.032904E-4	45.0	35
CGGCCTA	20	7.032904E-4	45.0	45
ATTACGC	25	3.890487E-5	45.0	18
AGCCACG	20	7.032904E-4	45.0	6
TAACGAC	25	3.890487E-5	45.0	23
CCGTACG	20	7.032904E-4	45.0	23
GCCGAAC	20	7.032904E-4	45.0	19
GTGTACG	50	2.1827873E-11	45.0	1
CTACCGC	20	7.032904E-4	45.0	25
GCGATAC	20	7.032904E-4	45.0	9
TATAGCG	50	2.1827873E-11	45.0	1
>>END_MODULE