FastQCFastQC Report
Thu 26 May 2016
SRR1545474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545474_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1439225
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.5124980458232729No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58890.4091785509562438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG302.1658852E-645.0000041
TGTAACG453.8562575E-1045.0000041
TACCGTA302.1658852E-645.00000444
CTATGCG207.03388E-445.01
TCGTTGC253.8912982E-545.012
CGCGATA253.8912982E-545.011
ATCGTAC253.8912982E-545.040
TAGTTCG207.03388E-445.01
TAGCACG406.8175723E-945.01
CCGTAGA207.03388E-445.026
CTCGAGA207.03388E-445.017
TTACGCC207.03388E-445.024
TACGTTC253.8912982E-545.031
TACGTAA207.03388E-445.044
TACCGAA207.03388E-445.09
ACGAACT207.03388E-445.045
CGTTAGG1850.042.5675662
CGTTTTT78600.042.02291
TCTAGCG650.041.5384641
TCACGAC603.6379788E-1241.25000425