##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545474_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1439225
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.074470635237716	31.0	31.0	33.0	30.0	34.0
2	31.362773020201846	31.0	31.0	34.0	30.0	34.0
3	31.43205892059963	31.0	31.0	34.0	30.0	34.0
4	35.27013288401744	37.0	35.0	37.0	33.0	37.0
5	35.14042488144661	37.0	35.0	37.0	32.0	37.0
6	35.21164932515764	37.0	35.0	37.0	32.0	37.0
7	35.69548889159096	37.0	35.0	37.0	35.0	37.0
8	35.65113029581893	37.0	35.0	37.0	35.0	37.0
9	37.415192898956036	39.0	37.0	39.0	35.0	39.0
10	36.81147978947003	39.0	37.0	39.0	32.0	39.0
11	36.43446090778023	38.0	35.0	39.0	32.0	39.0
12	35.5206989873022	37.0	35.0	39.0	31.0	39.0
13	34.98193263735691	37.0	34.0	39.0	29.0	39.0
14	35.94720700376939	38.0	34.0	40.0	30.0	41.0
15	36.3540120550991	38.0	35.0	40.0	31.0	41.0
16	36.50032065868783	38.0	35.0	40.0	31.0	41.0
17	36.434599176640205	38.0	35.0	40.0	31.0	41.0
18	36.3839781827025	37.0	35.0	40.0	31.0	41.0
19	36.29799440671194	37.0	35.0	40.0	31.0	41.0
20	36.145982038944574	36.0	35.0	40.0	31.0	41.0
21	35.94829925828137	36.0	34.0	40.0	31.0	41.0
22	35.840398130938524	36.0	34.0	40.0	30.0	41.0
23	35.80494571731314	36.0	34.0	40.0	30.0	41.0
24	35.69837933610103	36.0	34.0	40.0	30.0	41.0
25	35.58386562212302	36.0	34.0	40.0	30.0	41.0
26	35.47258489812225	35.0	34.0	40.0	30.0	41.0
27	35.346814431378	35.0	34.0	40.0	29.0	41.0
28	35.42883079435113	36.0	34.0	40.0	30.0	41.0
29	35.507799683857634	36.0	34.0	40.0	30.0	41.0
30	35.415789747954626	36.0	34.0	40.0	30.0	41.0
31	35.22141291319981	36.0	34.0	40.0	29.0	41.0
32	34.98476054821171	35.0	34.0	40.0	29.0	41.0
33	34.70177282912679	35.0	34.0	40.0	27.0	41.0
34	34.52031753200507	35.0	34.0	40.0	26.0	41.0
35	34.3139439628967	35.0	33.0	40.0	24.0	41.0
36	34.090487588806475	35.0	33.0	40.0	23.0	41.0
37	34.02052701975021	35.0	33.0	40.0	23.0	41.0
38	34.02812485886501	35.0	33.0	40.0	23.0	41.0
39	33.98450971877226	35.0	33.0	40.0	23.0	41.0
40	33.784675780367905	35.0	33.0	40.0	23.0	41.0
41	33.76987059007452	35.0	33.0	40.0	23.0	41.0
42	33.715915857492746	35.0	33.0	40.0	23.0	41.0
43	33.58766349945283	35.0	33.0	40.0	23.0	41.0
44	33.48806058816377	35.0	33.0	39.0	23.0	41.0
45	33.41705779151974	35.0	33.0	39.0	23.0	41.0
46	33.38328336431066	35.0	33.0	39.0	23.0	41.0
47	33.29519776268478	35.0	32.0	39.0	23.0	41.0
48	33.27221247546422	35.0	32.0	39.0	22.0	41.0
49	33.31123834007886	35.0	32.0	39.0	22.0	41.0
50	33.183978877520886	35.0	32.0	39.0	22.0	41.0
51	32.98325904566694	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	17.0
15	32.0
16	141.0
17	362.0
18	818.0
19	1674.0
20	2985.0
21	4657.0
22	7105.0
23	10371.0
24	15198.0
25	21874.0
26	28590.0
27	32791.0
28	35287.0
29	38723.0
30	45894.0
31	56184.0
32	70514.0
33	91714.0
34	154117.0
35	209619.0
36	101565.0
37	120120.0
38	163141.0
39	225643.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.73223436224357	24.098038875089024	28.36352898261217	16.80619778005524
2	31.703243064843928	26.06923865274714	29.713213708766872	12.514304573642065
3	26.925046465979957	24.966422901214194	34.37259636262572	13.735934270180131
4	24.828848859629314	27.923153085862186	32.865048897844325	14.382949156664177
5	21.763935451371395	32.40632979554969	32.44829682641699	13.381437926661919
6	20.9522486060206	40.056836144452745	28.81703694696799	10.173878302558668
7	86.00559328805434	4.492278830620647	8.091368618527332	1.4107592627976862
8	88.3590126630652	2.8600114645034656	7.556150011290799	1.2248258611405443
9	84.17245392485539	4.703434139901684	8.914936858378642	2.209175076864285
10	52.20594417134221	23.954211467977558	14.042488144661188	9.797356216019038
11	44.263405652347615	19.28718581180844	23.6697528183571	12.779655717486843
12	39.88188087338671	20.8156473101843	26.831801837794643	12.470669978634334
13	20.154666574024212	40.834754815959975	26.34473414511282	12.665844464902987
14	14.611474925741286	42.53372474769407	30.014973336344212	12.839826990220432
15	13.299240910906912	22.161024162309577	50.79171081658531	13.748024110198198
16	14.63621046049089	17.60822664975942	48.99880143827407	18.75676145147562
17	15.486112317393042	18.20278274765933	31.905643662387746	34.40546127255988
18	19.98103145790269	21.737462870642187	37.79548020636106	20.48602546509406
19	29.090725911514877	22.706734527262938	28.2497872118675	19.95275234935469
20	31.455331862634406	21.803679063384806	27.716236168771385	19.024752905209404
21	22.197502127881325	26.835901266306518	31.02794906981188	19.93864753600028
22	22.11877920408553	24.652364988101237	26.988761312511944	26.240094495301292
23	18.261425419930866	30.23731522173392	27.084785214264624	24.416474144070595
24	17.986207854921922	23.41482395038997	43.05046118570758	15.548507008980527
25	16.304122010109605	24.3731174764196	39.92377842241484	19.39898209105595
26	16.3448383678716	33.73190432350744	30.206395803296914	19.716861505324047
27	16.348173496152445	34.491827198665945	30.823741944449267	18.33625736073234
28	14.472024874498429	28.87227500911949	40.456912574475844	16.198787541906235
29	14.902325904566693	23.760635064010145	40.25715228682103	21.07988674460213
30	16.850388229776442	30.442078201810002	34.383782938734385	18.323750629679168
31	27.368062672618944	27.168302384964132	27.91453733780333	17.549097604613596
32	30.337160624641733	25.608990949990446	28.767079504594488	15.286768920773333
33	28.511108409039586	26.016501936806268	26.348555646267958	19.123834007886188
34	19.696920217478155	26.760721916309127	29.97432646042141	23.568031405791313
35	19.80552033212319	25.229898035401	33.40797998923031	21.556601643245497
36	30.123226041793327	24.878180965450156	27.094860080946344	17.903732911810174
37	20.25367819486182	33.31647240702462	29.297712310444858	17.13213708766871
38	20.194757595233543	33.78797616772916	26.1458771213674	19.871389115669892
39	19.710330212440724	33.39651548576491	28.152860046205426	18.740294255588946
40	24.19885702374542	26.28386805398739	27.16635689346697	22.350918028800223
41	17.265055846028243	25.24275217565009	28.35658079869374	29.135611179627922
42	20.881099202695896	25.235178655179002	25.88809949799371	27.99562264413139
43	21.263596727405375	26.791849780263682	27.534402195626118	24.410151296704825
44	18.92671403012038	30.13510743629384	30.239399676909446	20.698778856676338
45	16.31968594208689	37.47364032725946	25.326060900832044	20.880612829821604
46	20.277649429380396	33.58557557018534	26.959301012697807	19.177473987736455
47	21.23580399173166	28.103111049349476	27.715054977505254	22.946029981413606
48	22.316107627368897	24.862269624276955	32.23366742517674	20.587955323177404
49	20.77034515103615	24.82356823985131	34.307144470114125	20.098942138998417
50	19.24973510048811	34.55575049071549	27.658010387534958	18.536504021261443
51	18.0481856554743	34.630373985999405	25.290416717330505	22.031023641195784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	979.0
1	1533.0
2	2087.0
3	8106.0
4	14125.0
5	10779.5
6	7434.0
7	8012.5
8	8591.0
9	9186.5
10	9782.0
11	10221.5
12	10661.0
13	10334.5
14	10008.0
15	9260.5
16	8513.0
17	8417.0
18	8321.0
19	8036.5
20	7752.0
21	8050.5
22	8349.0
23	8176.5
24	8004.0
25	8965.0
26	11129.0
27	12332.0
28	14609.0
29	16886.0
30	21286.5
31	25687.0
32	29705.5
33	33724.0
34	38602.5
35	43481.0
36	45981.0
37	48481.0
38	58942.5
39	69404.0
40	88737.0
41	108070.0
42	128246.5
43	148423.0
44	148252.5
45	148082.0
46	136938.0
47	125794.0
48	116345.5
49	106897.0
50	102602.5
51	98308.0
52	91226.0
53	84144.0
54	77191.0
55	70238.0
56	62885.0
57	55532.0
58	47884.0
59	40236.0
60	36088.5
61	31941.0
62	27369.5
63	22798.0
64	18560.0
65	14322.0
66	11392.5
67	8463.0
68	6648.0
69	4833.0
70	3888.5
71	2944.0
72	2339.0
73	1734.0
74	1374.5
75	709.5
76	404.0
77	330.0
78	256.0
79	203.0
80	150.0
81	103.0
82	56.0
83	47.0
84	38.0
85	23.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1439225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.172331122468236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1076293903509	14.920870289623611
2	6.033110508477139	2.1927136371697857
3	1.833741069562775	0.9997004972689141
4	0.9086994452896553	0.660527488424273
5	0.6199598478822891	0.5633057819175998
6	0.4584804584422912	0.49989952223966105
7	0.36201266769808227	0.46050298475563284
8	0.3125007373234219	0.4543093499725352
9	0.2711897009643248	0.44353341386341694
>10	3.9572451051158857	17.471656633341578
>50	1.7300785242131387	23.191947868859017
>100	1.4011253426042947	36.832331177178105
>500	0.0034586198802629324	0.38234471295291084
>1k	0.0	0.0
>5k	7.68582195613985E-4	0.9263566424329428
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7376	0.5124980458232729	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5889	0.4091785509562438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012506731053170978	0.0
2	0.0	0.0	0.0	0.04606645937917977	0.0
3	0.0	0.0	0.0	0.06635515642099046	0.0
4	0.0	0.0	0.0	0.10248571279681773	0.0
5	0.0	0.0	0.0	0.17155066094599525	0.0
6	0.0	0.0	0.0	0.25749969601695355	0.0
7	0.0	0.0	0.0	0.3053032013757404	0.0
8	0.0	0.0	0.0	0.4289113932845802	0.0
9	0.0	0.0	0.0	0.486998210842641	0.0
10	0.0	0.0	0.0	0.5800343935103962	0.0
11	0.0	0.0	0.0	0.6775868957251299	0.0
12	0.0	0.0	0.0	0.7556150011290799	0.0
13	6.94818391842832E-5	0.0	0.0	0.7925793395751185	0.0
14	6.94818391842832E-5	0.0	0.0	0.8073789713213709	0.0
15	6.94818391842832E-5	0.0	0.0	0.8279455957199187	0.0
16	6.94818391842832E-5	0.0	0.0	0.875123764526047	0.0
17	6.94818391842832E-5	0.0	0.0	0.9304313085167364	0.0
18	6.94818391842832E-5	0.0	0.0	1.0145738157689035	0.0
19	6.94818391842832E-5	0.0	0.0	1.0516771178933106	0.0
20	6.94818391842832E-5	0.0	0.0	1.0932967395646962	0.0
21	6.94818391842832E-5	0.0	0.0	1.1436015911341173	0.0
22	6.94818391842832E-5	0.0	0.0	1.2030085636366794	0.0
23	6.94818391842832E-5	0.0	0.0	1.2705449113238028	0.0
24	6.94818391842832E-5	0.0	0.0	1.3258524553144921	0.0
25	6.94818391842832E-5	0.0	0.0	1.362608348242978	0.0
26	6.94818391842832E-5	0.0	0.0	1.4099254807274748	0.0
27	6.94818391842832E-5	0.0	0.0	1.4545328214837847	0.0
28	6.94818391842832E-5	0.0	0.0	1.4994875714360159	0.0
29	6.94818391842832E-5	0.0	0.0	1.549097604613594	0.0
30	6.94818391842832E-5	0.0	0.0	1.6220535357570915	0.0
31	1.389636783685664E-4	0.0	0.0	1.683961854470288	0.0
32	1.389636783685664E-4	0.0	0.0	1.7385745800691343	0.0
33	1.389636783685664E-4	0.0	0.0	1.7929788601504282	0.0
34	1.389636783685664E-4	0.0	0.0	1.8524553144921747	0.0
35	1.389636783685664E-4	0.0	0.0	1.9301360107002032	0.0
36	1.389636783685664E-4	0.0	0.0	1.9982282131008007	0.0
37	1.389636783685664E-4	0.0	0.0	2.0658340426271082	0.0
38	1.389636783685664E-4	0.0	0.0	2.1283676978929633	0.0
39	1.389636783685664E-4	0.0	0.0	2.1986833191474577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTG	30	2.1658852E-6	45.000004	1
TGTAACG	45	3.8562575E-10	45.000004	1
TACCGTA	30	2.1658852E-6	45.000004	44
CTATGCG	20	7.03388E-4	45.0	1
TCGTTGC	25	3.8912982E-5	45.0	12
CGCGATA	25	3.8912982E-5	45.0	11
ATCGTAC	25	3.8912982E-5	45.0	40
TAGTTCG	20	7.03388E-4	45.0	1
TAGCACG	40	6.8175723E-9	45.0	1
CCGTAGA	20	7.03388E-4	45.0	26
CTCGAGA	20	7.03388E-4	45.0	17
TTACGCC	20	7.03388E-4	45.0	24
TACGTTC	25	3.8912982E-5	45.0	31
TACGTAA	20	7.03388E-4	45.0	44
TACCGAA	20	7.03388E-4	45.0	9
ACGAACT	20	7.03388E-4	45.0	45
CGTTAGG	185	0.0	42.567566	2
CGTTTTT	7860	0.0	42.0229	1
TCTAGCG	65	0.0	41.538464	1
TCACGAC	60	3.6379788E-12	41.250004	25
>>END_MODULE