FastQCFastQC Report
Thu 26 May 2016
SRR1545473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545473_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1832468
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118610.6472691474012097No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44860.2448064577389619No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23530.12840606220681616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT253.8917166E-545.019
CACGTTA207.034387E-445.023
CACGCTA302.1662108E-644.99999635
CGTTTTT90950.041.56131
CGACGGT2350.041.1702128
ACCCGCT7100.040.5633834
GCTACGA3300.040.22727610
TCACGAC2350.040.21276525
CGGTCTA2300.040.10869231
ACGGGAT6550.039.847335
ATAGGGC7850.039.8407634
CACGACG2500.039.626
CGGAATA403.4597178E-739.37531
ACGTTAG403.4597178E-739.3751
CTCACGA2400.039.37499624
ATAGCGG2350.039.2553182
GCGCGAC7600.039.0789459
TCTACCG750.039.045
TAGGGCG10500.038.571435
TAGCCCG356.250324E-638.5714261
Produced by FastQC (version 0.11.5)