##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545473_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1832468 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.952638190680545 31.0 31.0 33.0 30.0 34.0 2 31.237027331445898 31.0 31.0 34.0 30.0 34.0 3 31.335710637238957 31.0 31.0 34.0 28.0 34.0 4 35.17950381671058 35.0 35.0 37.0 32.0 37.0 5 35.049442063926904 37.0 35.0 37.0 32.0 37.0 6 35.10206672094683 37.0 35.0 37.0 32.0 37.0 7 35.575718648292906 37.0 35.0 37.0 35.0 37.0 8 35.490355083963266 37.0 35.0 37.0 35.0 37.0 9 37.17385569625227 39.0 37.0 39.0 34.0 39.0 10 36.56919957128856 39.0 35.0 39.0 32.0 39.0 11 36.31464996933098 38.0 35.0 39.0 32.0 39.0 12 35.73550534033882 37.0 35.0 39.0 31.0 39.0 13 35.33866512266517 37.0 35.0 39.0 30.0 39.0 14 36.38819668338001 38.0 35.0 40.0 30.0 41.0 15 36.69129228996086 38.0 35.0 40.0 31.0 41.0 16 36.74781824293794 38.0 35.0 40.0 31.0 41.0 17 36.64030531501778 38.0 35.0 40.0 31.0 41.0 18 36.59537956460904 38.0 35.0 40.0 31.0 41.0 19 36.49213628832809 38.0 35.0 40.0 31.0 41.0 20 36.33570518011774 38.0 35.0 40.0 31.0 41.0 21 36.19015557161162 38.0 34.0 40.0 30.0 41.0 22 36.08490571185963 38.0 34.0 40.0 30.0 41.0 23 35.97838434286438 37.0 34.0 40.0 30.0 41.0 24 35.90921915143948 37.0 34.0 40.0 30.0 41.0 25 35.758390869581355 37.0 34.0 40.0 30.0 41.0 26 35.62062257021678 37.0 34.0 40.0 29.0 41.0 27 35.5211419790141 37.0 34.0 40.0 29.0 41.0 28 35.54519369506043 37.0 34.0 40.0 29.0 41.0 29 35.57460484985277 37.0 34.0 40.0 29.0 41.0 30 35.46310876915722 37.0 34.0 40.0 29.0 41.0 31 35.330910007705455 37.0 34.0 40.0 29.0 41.0 32 35.099667770460385 37.0 34.0 40.0 27.0 41.0 33 34.85255185902292 37.0 33.0 40.0 26.0 41.0 34 34.67265185531207 37.0 34.0 40.0 25.0 41.0 35 34.499434096529924 37.0 33.0 40.0 24.0 41.0 36 34.317036914150755 36.0 33.0 40.0 23.0 41.0 37 34.250105868151586 36.0 33.0 40.0 23.0 41.0 38 34.22753412337896 36.0 33.0 40.0 23.0 41.0 39 34.1411227917759 36.0 33.0 40.0 23.0 41.0 40 33.98584804755117 36.0 33.0 40.0 23.0 41.0 41 33.91092668466789 36.0 33.0 40.0 23.0 41.0 42 33.84806064826234 36.0 33.0 40.0 23.0 41.0 43 33.753382323729525 36.0 33.0 40.0 23.0 41.0 44 33.68746575656437 35.0 33.0 40.0 23.0 41.0 45 33.65096907558549 35.0 33.0 40.0 23.0 41.0 46 33.55324731455065 35.0 32.0 40.0 23.0 41.0 47 33.45295688655955 35.0 32.0 40.0 22.0 41.0 48 33.42293944560014 35.0 32.0 39.0 22.0 41.0 49 33.41733061641458 35.0 32.0 39.0 22.0 41.0 50 33.33884357052892 35.0 32.0 39.0 22.0 41.0 51 33.14405654014149 35.0 32.0 39.0 21.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 6.0 14 27.0 15 53.0 16 208.0 17 574.0 18 1301.0 19 2551.0 20 4337.0 21 6802.0 22 10163.0 23 14766.0 24 20512.0 25 27516.0 26 35547.0 27 41085.0 28 45194.0 29 49612.0 30 58324.0 31 70320.0 32 86526.0 33 109644.0 34 168438.0 35 219025.0 36 145970.0 37 173205.0 38 231007.0 39 309616.0 40 137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.01356422049389 23.588570168756014 27.2068052484409 16.19106036230919 2 31.597332122580042 26.832010163342552 28.507073520519867 13.06358419355754 3 28.90740793290797 25.7187028641155 32.40373092463279 12.97015827834374 4 26.204168367469443 27.894020523141467 31.134022531362078 14.76778857802701 5 23.228072741242958 31.11574117528928 30.91169941303204 14.74448667043572 6 21.475190835528917 40.769770604452575 27.84201415795528 9.913024402063227 7 84.12463410002249 4.99877760484767 8.862473996817407 2.01411429831244 8 84.60617047610108 4.688485692519596 8.519712213255566 2.1856316181237543 9 79.3453964816848 6.3846681088018995 10.437562893322012 3.8323725161912785 10 43.96922620204009 25.72890768078897 17.30813307517512 12.993733041995823 11 38.31630347705936 24.581220517902633 23.331048618584337 13.77142738645368 12 36.195011318069405 21.670774059901728 26.88412567095305 15.250088951075815 13 22.144506752641792 34.75678702165604 26.172025923508624 16.926680302193546 14 16.37114536242925 37.75776712062639 28.405079924997327 17.466007591947037 15 16.158317635014637 24.84409004686576 42.4858715131724 16.51172080494721 16 17.338856667619844 19.741790852555134 42.33012527367463 20.589227206150394 17 17.015303950737476 21.211502738383427 31.3261131981568 30.447080112722297 18 20.804619780536413 24.08931561151409 34.41337038354831 20.69269422440119 19 28.420905576523026 24.86073426657382 26.903716736117627 19.81464342078552 20 31.16529183592838 21.9914890737519 27.723267200300363 19.11995189001936 21 23.224307327604084 27.61647133810795 28.672151437296584 20.48706989699138 22 23.261634036719876 25.23874905318947 26.609796187436835 24.88982072265382 23 21.33739852483099 29.54414483636276 26.456232796425365 22.662223842380875 24 20.06217843913236 24.1788669706647 37.38013433249584 18.378820257707094 25 19.26118218708321 26.38223423273967 34.37457025170426 19.98201332847286 26 18.615441033622414 32.07559422592918 28.405461923482427 20.903502816965972 27 19.155259464285322 32.58900018990782 28.645411543339367 19.610328802467492 28 17.254980714533623 29.15314210125361 35.44864084939 18.143236334822763 29 17.916492948307965 25.361807136604842 35.1136827491667 21.608017165920497 30 19.15667831580142 30.385360071772062 31.125400279841177 19.332561332585342 31 26.246188200830794 28.04496449596937 25.901298139994804 19.80754916320503 32 27.855984388267625 26.59315196772877 27.463617372854532 18.087246271149073 33 26.386981928197383 27.539034788056327 26.162967102290462 19.911016181455828 34 20.217979249842287 27.802068030655924 28.368408070427424 23.61154464907436 35 21.332323402100336 25.651198274676556 30.297227564137547 22.71925075908556 36 26.52210024949958 28.050803615670233 25.93567800365409 19.491418131176097 37 20.631847322845474 32.388178129167876 28.64939524182687 18.33057930615978 38 20.78852127294992 31.805139298476153 25.92994802637754 21.47639140219638 39 20.942248377597863 31.419320828521975 26.75926673753648 20.879164056343686 40 23.294322192802277 26.51238657373553 28.39771281135605 21.795578422106143 41 17.890462480108795 26.172953634115302 29.724830119816552 26.21175376595935 42 21.210520456564588 27.376521718251013 25.955214497606505 25.45774332757789 43 21.0643241791944 28.109031099042387 27.952793718635192 22.87385100312802 44 19.923512989039917 29.63762532278872 28.62347391605201 21.815387772119347 45 18.25920016065765 34.60300534579594 25.075853984899055 22.061940508647353 46 19.531964541809188 33.010071662915806 26.821587061820452 20.636376733454554 47 21.03005345795943 29.63462390612005 27.377285715221223 21.958036920699296 48 21.903247423693074 26.196146399282277 30.365005009637276 21.53560116738737 49 19.87467175415887 27.998251538362474 30.48980937184169 21.637267335636967 50 19.587136037300514 32.154886197194166 27.506837772883348 20.751139992621972 51 18.65069403667622 32.21546024268909 26.29857656450208 22.835269156132604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1944.0 1 2800.5 2 3657.0 3 12572.0 4 21487.0 5 16004.5 6 10522.0 7 10890.0 8 11258.0 9 11942.0 10 12626.0 11 12716.5 12 12807.0 13 12402.0 14 11997.0 15 11692.5 16 11388.0 17 10802.5 18 10217.0 19 9685.5 20 9154.0 21 9548.0 22 9942.0 23 10135.5 24 10329.0 25 11726.5 26 14644.5 27 16165.0 28 21687.5 29 27210.0 30 31883.0 31 36556.0 32 41097.5 33 45639.0 34 50168.0 35 54697.0 36 56042.5 37 57388.0 38 66288.0 39 75188.0 40 94001.5 41 112815.0 42 133314.5 43 153814.0 44 154601.0 45 155388.0 46 149983.0 47 144578.0 48 137694.0 49 130810.0 50 125413.5 51 120017.0 52 111515.0 53 103013.0 54 97518.5 55 92024.0 56 90264.5 57 88505.0 58 84520.5 59 80536.0 60 73780.5 61 67025.0 62 56226.0 63 45427.0 64 37364.5 65 29302.0 66 23521.0 67 17740.0 68 14302.0 69 10864.0 70 8845.0 71 6826.0 72 5736.0 73 4646.0 74 3609.0 75 2088.0 76 1604.0 77 1244.5 78 885.0 79 686.5 80 488.0 81 333.0 82 178.0 83 119.0 84 60.0 85 40.0 86 20.0 87 18.5 88 17.0 89 13.5 90 10.0 91 8.5 92 7.0 93 4.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1832468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.44372390367511 #Duplication Level Percentage of deduplicated Percentage of total 1 80.42177387068742 15.636987669854394 2 5.981724925221691 2.326140158274844 3 2.1705324114338906 1.2660969879569615 4 1.213036040458765 0.9434375142354998 5 0.8221384280981834 0.7992716303271262 6 0.6048582962483355 0.7056418627859977 7 0.477802270958874 0.6503178805951316 8 0.4140964267081832 0.6441261272329879 9 0.3520614078223769 0.6160846329753714 >10 4.768284452813646 22.212689369664982 >50 1.7556493455722935 24.939964421284483 >100 1.0112777244822553 27.252552087441416 >500 0.004227749683887403 0.5190309895665681 >1k 0.0022547998314066146 0.8376505834151613 >5k 0.0 0.0 >10k+ 2.818499789258268E-4 0.6500080843890893 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11861 0.6472691474012097 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 4486 0.2448064577389619 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2353 0.12840606220681616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.022647053045401065 0.0 2 0.0 0.0 0.0 0.0802742530838192 0.0 3 0.0 0.0 0.0 0.11083413189207124 0.0 4 0.0 0.0 0.0 0.1620219288958934 0.0 5 0.0 0.0 0.0 0.2709460683624489 0.0 6 0.0 0.0 0.0 0.3976604229923797 0.0 7 0.0 0.0 0.0 0.4735689791035914 0.0 8 0.0 0.0 0.0 0.6857418519723127 0.0 9 0.0 0.0 0.0 0.7856071702207078 0.0 10 5.457121215759293E-5 0.0 0.0 0.9671110218568619 0.0 11 5.457121215759293E-5 0.0 0.0 1.1026113416441652 0.0 12 5.457121215759293E-5 0.0 0.0 1.2374568068855774 0.0 13 5.457121215759293E-5 0.0 0.0 1.2904454538906 0.0 14 5.457121215759293E-5 0.0 0.0 1.3125467948144252 0.0 15 5.457121215759293E-5 0.0 0.0 1.3458352342305568 0.0 16 5.457121215759293E-5 0.0 0.0 1.4087558418482615 0.0 17 5.457121215759293E-5 0.0 0.0 1.4785524221978228 0.0 18 5.457121215759293E-5 0.0 0.0 1.5827288662066676 0.0 19 5.457121215759293E-5 0.0 0.0 1.6334255223010716 0.0 20 1.0914242431518586E-4 0.0 0.0 1.684831604153524 0.0 21 1.0914242431518586E-4 0.0 0.0 1.7466607875280769 0.0 22 1.0914242431518586E-4 0.0 0.0 1.811927957268558 0.0 23 1.0914242431518586E-4 0.0 0.0 1.8882185118648729 0.0 24 1.0914242431518586E-4 0.0 0.0 1.9488471285719586 0.0 25 1.0914242431518586E-4 0.0 0.0 1.9991072149691018 0.0 26 1.0914242431518586E-4 0.0 0.0 2.0469115968191534 0.0 27 1.0914242431518586E-4 0.0 0.0 2.09913624685397 0.0 28 1.0914242431518586E-4 0.0 0.0 2.154853454466872 0.0 29 1.637136364727788E-4 0.0 0.0 2.2099158075338834 0.0 30 1.637136364727788E-4 0.0 0.0 2.28866206667729 0.0 31 1.637136364727788E-4 0.0 0.0 2.3581312197539055 0.0 32 1.637136364727788E-4 0.0 0.0 2.424435242525381 0.0 33 1.637136364727788E-4 0.0 0.0 2.4915578334792206 0.0 34 1.637136364727788E-4 0.0 0.0 2.558625853220902 0.0 35 1.637136364727788E-4 0.0 0.0 2.6431566608530135 0.0 36 1.637136364727788E-4 0.0 0.0 2.7166095124171337 0.0 37 1.637136364727788E-4 0.0 0.0 2.79355492155934 0.0 38 1.637136364727788E-4 0.0 0.0 2.864661211000683 0.0 39 1.637136364727788E-4 0.0 0.0 2.9364769262000756 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCGT 25 3.8917166E-5 45.0 19 CACGTTA 20 7.034387E-4 45.0 23 CACGCTA 30 2.1662108E-6 44.999996 35 CGTTTTT 9095 0.0 41.5613 1 CGACGGT 235 0.0 41.17021 28 ACCCGCT 710 0.0 40.56338 34 GCTACGA 330 0.0 40.227276 10 TCACGAC 235 0.0 40.212765 25 CGGTCTA 230 0.0 40.108692 31 ACGGGAT 655 0.0 39.84733 5 ATAGGGC 785 0.0 39.840763 4 CACGACG 250 0.0 39.6 26 CGGAATA 40 3.4597178E-7 39.375 31 ACGTTAG 40 3.4597178E-7 39.375 1 CTCACGA 240 0.0 39.374996 24 ATAGCGG 235 0.0 39.255318 2 GCGCGAC 760 0.0 39.078945 9 TCTACCG 75 0.0 39.0 45 TAGGGCG 1050 0.0 38.57143 5 TAGCCCG 35 6.250324E-6 38.571426 1 >>END_MODULE