FastQCFastQC Report
Thu 26 May 2016
SRR1545472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545472_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1255486
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75480.6012014470890157No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30400.24213730778359935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTA253.891011E-545.00000423
GAACGGC253.891011E-545.0000043
ACGTCGT253.891011E-545.00000432
AGTCGGT253.891011E-545.00000412
GCGATCG207.0335355E-445.09
TCGCGGA207.0335355E-445.023
TACGGGA1800.042.4999964
CGTTTTT66850.041.970831
GCGATAC650.041.5384649
TCGACAA1900.041.4473719
CGAGGGC3500.039.8571434
TCATCGA2000.039.37500416
ACGGGAC1600.039.3755
ATCGATA403.4585537E-739.37540
GCGATGC2200.038.863649
TGGGCGA8250.038.7272726
CGACGGT700.038.5714328
ACCGAAT356.248749E-638.5714326
CGCAGAT356.248749E-638.5714327
GCGATGA2900.038.017249
Produced by FastQC (version 0.11.5)