##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545472_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1255486
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.905035978099317	31.0	31.0	33.0	28.0	34.0
2	31.200766874341888	31.0	31.0	34.0	28.0	34.0
3	31.27631291786607	31.0	31.0	34.0	28.0	34.0
4	35.11732588017708	35.0	35.0	37.0	32.0	37.0
5	34.98749328945126	35.0	35.0	37.0	32.0	37.0
6	35.036717255309895	37.0	35.0	37.0	32.0	37.0
7	35.55386758593883	37.0	35.0	37.0	35.0	37.0
8	35.469176080020006	37.0	35.0	37.0	35.0	37.0
9	37.15518452615163	39.0	37.0	39.0	34.0	39.0
10	36.52950650186462	39.0	35.0	39.0	32.0	39.0
11	36.22633306942491	38.0	35.0	39.0	31.0	39.0
12	35.48765020079873	37.0	35.0	39.0	30.0	39.0
13	35.05289585069049	37.0	34.0	39.0	29.0	39.0
14	35.9830312723519	38.0	34.0	40.0	29.0	41.0
15	36.339854048551715	38.0	35.0	40.0	30.0	41.0
16	36.436616577166134	38.0	35.0	40.0	31.0	41.0
17	36.35448025704787	38.0	35.0	40.0	31.0	41.0
18	36.27310220902503	38.0	35.0	40.0	30.0	41.0
19	36.18871735726245	37.0	35.0	40.0	30.0	41.0
20	36.05788435713341	37.0	34.0	40.0	30.0	41.0
21	35.868491564222936	37.0	34.0	40.0	30.0	41.0
22	35.77698437099259	37.0	34.0	40.0	30.0	41.0
23	35.71080760757189	37.0	34.0	40.0	30.0	41.0
24	35.5849312537137	36.0	34.0	40.0	29.0	41.0
25	35.44696794707388	36.0	34.0	40.0	29.0	41.0
26	35.30852753435721	36.0	34.0	40.0	29.0	41.0
27	35.19369869516665	36.0	34.0	40.0	28.0	41.0
28	35.228654879465005	36.0	34.0	40.0	28.0	41.0
29	35.25446480486441	36.0	34.0	40.0	29.0	41.0
30	35.128970773071146	36.0	34.0	40.0	27.0	41.0
31	34.94953428393467	36.0	34.0	40.0	27.0	41.0
32	34.73904607458785	36.0	33.0	40.0	26.0	41.0
33	34.4918254763494	36.0	33.0	40.0	24.0	41.0
34	34.2867813739062	36.0	33.0	40.0	24.0	41.0
35	34.113530537178434	36.0	33.0	40.0	23.0	41.0
36	33.91711496583793	36.0	33.0	40.0	23.0	41.0
37	33.85944964738754	36.0	33.0	40.0	23.0	41.0
38	33.810306924967705	35.0	33.0	40.0	23.0	41.0
39	33.74530819140954	35.0	33.0	40.0	23.0	41.0
40	33.58495913136427	35.0	32.0	40.0	22.0	41.0
41	33.538841532283115	35.0	32.0	40.0	21.0	41.0
42	33.503050611476354	35.0	32.0	40.0	22.0	41.0
43	33.38680638414128	35.0	32.0	40.0	21.0	41.0
44	33.31575103187132	35.0	32.0	40.0	21.0	41.0
45	33.29032661455405	35.0	32.0	39.0	22.0	41.0
46	33.2360814855761	35.0	32.0	39.0	22.0	41.0
47	33.15422872098932	35.0	32.0	39.0	21.0	41.0
48	33.10326280022238	35.0	32.0	39.0	21.0	41.0
49	33.12156487607189	35.0	32.0	39.0	20.0	41.0
50	33.01397865049869	35.0	32.0	39.0	20.0	41.0
51	32.82923903572003	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	16.0
14	18.0
15	70.0
16	213.0
17	525.0
18	1209.0
19	2321.0
20	3726.0
21	5687.0
22	8176.0
23	11551.0
24	15556.0
25	21024.0
26	26793.0
27	31029.0
28	32814.0
29	36649.0
30	42301.0
31	50627.0
32	61767.0
33	77412.0
34	119859.0
35	154717.0
36	94798.0
37	112410.0
38	149519.0
39	194646.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.76349875665679	23.584412729413152	26.43996030222559	16.21212821170447
2	33.911728207244046	24.59167206962085	27.807876790342544	13.68872293279256
3	28.789170090307657	24.802666059199385	32.68208486594036	13.726078984552595
4	26.059470197198536	27.361834381267492	31.188639299840858	15.390056121693114
5	23.140680182813668	30.810618358149753	30.980512725749232	15.06818873328735
6	21.54145884541922	39.15830204398934	28.877024514809403	10.423214595782031
7	83.43828605018295	5.1592769652548895	9.307789971373635	2.094647013188518
8	84.2108155726149	4.401562422838646	9.123239924618833	2.2643820799276138
9	79.57093906264188	5.744707627165894	10.994547131549059	3.689806178643171
10	47.56986537484289	22.322112711730757	17.473711375515137	12.634310537911214
11	41.37776128128868	23.43387341635032	22.3534153307962	12.834949971564797
12	35.950619919298184	22.9418727090545	27.119059870042356	13.988447501604956
13	22.07862134663389	35.941221168535535	27.37736621515493	14.602791269675647
14	17.179403035955797	37.58448919382614	30.450678064112225	14.785429706105843
15	16.432600602475855	23.150238234436703	45.6506086089371	14.766552554150344
16	18.467669093880776	20.238298157048344	43.17005526146846	18.12397748760241
17	19.50798336261814	19.33020360242966	30.93359862236616	30.22821441258604
18	22.661025292197603	23.23227817753444	34.79313986774842	19.313556662519535
19	29.187262940407145	23.37039202348732	28.46897536093592	18.973369675169614
20	30.19133626340716	23.588873153503904	27.444670828667146	18.775119754421794
21	24.009427424917522	26.616624956391387	30.706037343307692	18.6679102753834
22	23.99445314404143	24.46056746152486	27.751484285766626	23.79349510866708
23	20.361039469974177	28.762487196193348	28.219749164865238	22.656724168967237
24	19.311087499183582	24.510986183836376	38.17788489875634	18.0000414182237
25	19.382215333345016	24.894662306071115	36.127762476045135	19.595359884538734
26	18.228000949433127	31.19182531704854	29.4952711539595	21.084902579558833
27	19.134104243297017	30.88883508059827	30.90787153341415	19.06918914269056
28	16.954549871523856	28.0445182184429	36.630993893998024	18.369938016035224
29	18.201955258760353	25.165394118293634	36.666677286724024	19.96597333622199
30	19.75059857298289	28.743211792086885	32.822588224799006	18.683601410131214
31	27.112528534766618	26.518017723813724	28.054315221356514	18.315138520063144
32	28.81808319646734	25.062485762485604	28.200075508607824	17.91935553243923
33	27.343833384044107	26.013193297256997	27.262988197399252	19.37998512129964
34	21.04077624123248	26.684168521194184	30.049797448956024	22.225257788617316
35	20.96056825802916	26.291730851638327	30.994610851893214	21.7530900384393
36	28.375226804599972	26.039238987929775	27.348771710716008	18.236762496754245
37	21.485145991273498	31.470044269709103	28.51525226087746	18.529557478139942
38	21.367900558030914	32.321188766740526	25.695308430360832	20.615602244867727
39	21.149817680165288	31.425201077511016	26.703204974010063	20.721776268313626
40	23.974460886063245	26.637095116950725	26.65390135772123	22.734542639264795
41	19.05429451224466	25.647916424396612	27.792185655594725	27.505603407764006
42	22.15675841865222	25.77631291786607	26.571622463332922	25.495306200148786
43	22.011635334842445	26.895401462063294	27.414881567775346	23.67808163531891
44	19.745023042869455	29.920285849463873	29.168465438881835	21.16622566878484
45	18.853495777730693	33.790261301201284	25.663527908714233	21.69271501235378
46	21.192589961178378	31.798044741239647	26.71459498552752	20.294770312054457
47	21.397291566771752	28.498207068816377	28.45981556146385	21.644685802948022
48	22.40455090697945	26.356884903535367	30.64821113098832	20.59035305849687
49	21.350297812958487	26.417100628760497	31.586811800370533	20.64578975791048
50	20.154346603626006	32.59654030391418	27.293175710442014	19.9559373820178
51	19.29635216959807	32.777426430880155	26.33529963695334	21.590921762568442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1016.0
1	1719.5
2	2423.0
3	8394.0
4	14365.0
5	10688.0
6	7011.0
7	7228.0
8	7445.0
9	7744.5
10	8044.0
11	7913.5
12	7783.0
13	7836.5
14	7890.0
15	7620.0
16	7350.0
17	7015.0
18	6680.0
19	6455.5
20	6231.0
21	6459.5
22	6688.0
23	6904.5
24	7121.0
25	8191.0
26	10255.0
27	11249.0
28	13415.5
29	15582.0
30	19096.5
31	22611.0
32	23904.0
33	25197.0
34	28896.0
35	32595.0
36	36844.0
37	41093.0
38	47052.5
39	53012.0
40	66662.0
41	80312.0
42	94716.5
43	109121.0
44	110883.0
45	112645.0
46	107565.5
47	102486.0
48	99153.0
49	95820.0
50	92556.5
51	89293.0
52	83073.5
53	76854.0
54	72156.5
55	67459.0
56	62613.5
57	57768.0
58	52477.0
59	47186.0
60	42573.0
61	37960.0
62	32648.5
63	27337.0
64	23146.0
65	18955.0
66	16253.0
67	13551.0
68	10524.0
69	7497.0
70	5937.0
71	4377.0
72	3545.0
73	2713.0
74	2151.5
75	1256.0
76	922.0
77	672.5
78	423.0
79	356.5
80	290.0
81	196.0
82	102.0
83	107.5
84	113.0
85	77.0
86	41.0
87	29.0
88	17.0
89	10.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1255486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.825843080787177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.72322031352486	15.805800558309684
2	5.8221370409844635	2.308575507387931
3	2.269590346518544	1.3498962620323813
4	1.2855973022843237	1.0195220152068925
5	0.8354415818647442	0.8281666852607515
6	0.6262986811530707	0.7450139624546843
7	0.5327995098252406	0.7394239632720883
8	0.4197569269541542	0.6657627972693204
9	0.3889980316280361	0.6940992540413276
>10	5.085120652487987	24.256161085328163
>50	2.2850593279828573	32.430074697852646
>100	0.7239591815489885	18.131869552927547
>500	0.0012126619456431969	0.1771097347734821
>1k	4.042206485477323E-4	0.24362606050288943
>5k	4.042206485477323E-4	0.6048978633802005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7548	0.6012014470890157	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3040	0.24213730778359935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006292383985165904	0.0
2	0.0	0.0	0.0	0.023576527336824145	0.0
3	0.0	0.0	0.0	0.034807237993892404	0.0
4	0.0	0.0	0.0	0.05718900887783695	0.0
5	0.0	0.0	0.0	0.0972531752643996	0.0
6	0.0	0.0	0.0	0.15396428156108471	0.0
7	0.0	0.0	0.0	0.18765641353228948	0.0
8	0.0	0.0	0.0	0.2966182020349092	0.0
9	0.0	0.0	0.0	0.3472758756370043	0.0
10	0.0	0.0	0.0	0.4252536467949463	0.0
11	0.0	0.0	0.0	0.48307985911431905	0.0
12	0.0	0.0	0.0	0.5363659969127493	0.0
13	0.0	0.0	0.0	0.5598628738193815	0.0
14	0.0	0.0	0.0	0.5701377793141461	0.0
15	0.0	0.0	0.0	0.5812091891108304	0.0
16	0.0	0.0	0.0	0.6081310345157174	0.0
17	0.0	0.0	0.0	0.6374423928263636	0.0
18	0.0	0.0	0.0	0.6890558715907624	0.0
19	0.0	0.0	0.0	0.711437642474707	0.0
20	0.0	0.0	0.0	0.7359699749738349	0.0
21	0.0	0.0	0.0	0.7674318948996643	0.0
22	0.0	0.0	0.0	0.8005664738595253	0.0
23	0.0	0.0	0.0	0.8366481187364893	0.0
24	0.0	0.0	0.0	0.8667559813490553	0.0
25	0.0	0.0	0.0	0.8939964284747102	0.0
26	7.965043019197347E-5	0.0	0.0	0.9229891850645885	0.0
27	7.965043019197347E-5	0.0	0.0	0.9525394946658107	0.0
28	7.965043019197347E-5	0.0	0.0	0.981372950395305	0.0
29	1.5930086038394695E-4	0.0	0.0	1.0164191396797735	0.0
30	1.5930086038394695E-4	0.0	0.0	1.0671564637120605	0.0
31	1.5930086038394695E-4	0.0	0.0	1.1100880455855342	0.0
32	1.5930086038394695E-4	0.0	0.0	1.152541724877856	0.0
33	1.5930086038394695E-4	0.0	0.0	1.194836103309794	0.0
34	1.5930086038394695E-4	0.0	0.0	1.2376880347530756	0.0
35	1.5930086038394695E-4	0.0	0.0	1.2967090035253281	0.0
36	2.389512905759204E-4	0.0	0.0	1.3464108719651195	0.0
37	2.389512905759204E-4	0.0	0.0	1.3969092447068308	0.0
38	3.186017207678939E-4	0.0	0.0	1.4553726604677393	0.0
39	3.186017207678939E-4	0.0	0.0	1.5720605406989803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGTA	25	3.891011E-5	45.000004	23
GAACGGC	25	3.891011E-5	45.000004	3
ACGTCGT	25	3.891011E-5	45.000004	32
AGTCGGT	25	3.891011E-5	45.000004	12
GCGATCG	20	7.0335355E-4	45.0	9
TCGCGGA	20	7.0335355E-4	45.0	23
TACGGGA	180	0.0	42.499996	4
CGTTTTT	6685	0.0	41.97083	1
GCGATAC	65	0.0	41.538464	9
TCGACAA	190	0.0	41.44737	19
CGAGGGC	350	0.0	39.857143	4
TCATCGA	200	0.0	39.375004	16
ACGGGAC	160	0.0	39.375	5
ATCGATA	40	3.4585537E-7	39.375	40
GCGATGC	220	0.0	38.86364	9
TGGGCGA	825	0.0	38.727272	6
CGACGGT	70	0.0	38.57143	28
ACCGAAT	35	6.248749E-6	38.57143	26
CGCAGAT	35	6.248749E-6	38.57143	27
GCGATGA	290	0.0	38.01724	9
>>END_MODULE