FastQCFastQC Report
Thu 26 May 2016
SRR1545471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545471_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1558531
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164131.0531070604306236No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA17630.11311934122580816No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17480.11215689646211721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG207.034061E-445.013
TTAACGC207.034061E-445.030
CGTTTTT127250.042.1178781
TCGTAAG950.040.2631571
ACGGGAC2650.039.0566025
GGGCGAT33300.038.918927
GGCGATA5750.038.7391328
CGCGTCA356.24972E-638.5714339
GGTACCT8550.037.8947378
ACGGGTA1800.037.55
GGGTACC10300.037.354377
GCGCGAC4650.036.7741939
AGGGCGA18750.036.726
GACACGA4800.036.56250425
ATAGGGC9000.036.54
AACACGT4750.036.47368241
TAGCCGT1550.036.2903244
TACGGGT1800.036.254
GTTTTTT155400.036.1534772
GACCGAT8550.036.052639
Produced by FastQC (version 0.11.5)