##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545471_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1558531 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84512531351638 31.0 31.0 33.0 28.0 34.0 2 31.140837108790265 31.0 31.0 34.0 28.0 34.0 3 31.198840446548704 31.0 31.0 34.0 28.0 34.0 4 35.0739722212776 35.0 35.0 37.0 32.0 37.0 5 34.963810793625534 35.0 35.0 37.0 32.0 37.0 6 35.0144674696878 37.0 35.0 37.0 32.0 37.0 7 35.528844148752896 37.0 35.0 37.0 35.0 37.0 8 35.441844916783815 37.0 35.0 37.0 35.0 37.0 9 37.12557530135749 39.0 37.0 39.0 34.0 39.0 10 36.45526588819857 38.0 35.0 39.0 32.0 39.0 11 36.2224909225418 38.0 35.0 39.0 31.0 39.0 12 35.832070071111836 37.0 35.0 39.0 30.0 39.0 13 35.563850831327706 37.0 35.0 39.0 30.0 39.0 14 36.599791726953136 38.0 35.0 40.0 30.0 41.0 15 36.81076988523167 38.0 35.0 40.0 31.0 41.0 16 36.7832619306257 38.0 35.0 40.0 31.0 41.0 17 36.66928408867068 38.0 35.0 40.0 31.0 41.0 18 36.557233061132564 38.0 35.0 40.0 31.0 41.0 19 36.43810485643211 38.0 35.0 40.0 30.0 41.0 20 36.28960476243334 38.0 35.0 40.0 30.0 41.0 21 36.13636751530768 38.0 34.0 40.0 30.0 41.0 22 36.03935564964701 38.0 34.0 40.0 30.0 41.0 23 35.93565735939805 38.0 34.0 40.0 30.0 41.0 24 35.81436044583008 37.0 34.0 40.0 29.0 41.0 25 35.66505318148949 37.0 34.0 40.0 29.0 41.0 26 35.53300832643047 37.0 34.0 40.0 29.0 41.0 27 35.42849131650253 37.0 34.0 40.0 28.0 41.0 28 35.41789864943335 37.0 34.0 40.0 28.0 41.0 29 35.386712872570385 37.0 34.0 40.0 28.0 41.0 30 35.21534701587585 37.0 34.0 40.0 27.0 41.0 31 35.04076466878105 37.0 34.0 40.0 26.0 41.0 32 34.77194101368532 37.0 33.0 40.0 25.0 41.0 33 34.45536598245399 37.0 33.0 40.0 23.0 41.0 34 34.1927308471888 37.0 33.0 40.0 23.0 41.0 35 33.94743896656531 37.0 33.0 40.0 21.0 41.0 36 33.78218655900974 37.0 33.0 40.0 21.0 41.0 37 33.73918645185755 37.0 33.0 40.0 21.0 41.0 38 33.645278149744854 36.0 32.0 40.0 20.0 41.0 39 33.53199070150032 36.0 32.0 40.0 19.0 41.0 40 33.39721956124068 36.0 32.0 40.0 18.0 41.0 41 33.269198366923725 36.0 32.0 40.0 18.0 41.0 42 33.203148990940825 36.0 32.0 40.0 18.0 41.0 43 33.09250120786818 36.0 31.0 40.0 18.0 41.0 44 32.98713211350945 35.0 31.0 40.0 18.0 41.0 45 32.89228254041787 35.0 31.0 40.0 18.0 41.0 46 32.82190601277742 35.0 31.0 39.0 18.0 41.0 47 32.71935816483599 35.0 31.0 39.0 18.0 41.0 48 32.650237948427076 35.0 31.0 39.0 18.0 41.0 49 32.65278265238228 35.0 31.0 39.0 18.0 41.0 50 32.585506480140594 35.0 31.0 39.0 18.0 41.0 51 32.40649624550298 35.0 31.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 3.0 12 6.0 13 10.0 14 32.0 15 84.0 16 275.0 17 743.0 18 1582.0 19 2987.0 20 4926.0 21 7452.0 22 10828.0 23 15158.0 24 20836.0 25 28714.0 26 37110.0 27 42340.0 28 43621.0 29 46297.0 30 52875.0 31 61525.0 32 73432.0 33 90018.0 34 126996.0 35 154337.0 36 132017.0 37 157851.0 38 204902.0 39 241522.0 40 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.642898344659166 22.21110776750671 24.973709217205176 19.172284670628944 2 35.902590323836996 23.53164614627492 27.135680971376253 13.43008255851183 3 28.454101971664343 24.273370244159402 33.88075052725932 13.391777256916932 4 25.05834019342573 26.90867233311368 32.79350875920979 15.239478714250792 5 22.036841102294407 30.64590951350984 32.888277486941234 14.42897189725453 6 20.254393399938788 38.94956211971402 30.957035824118996 9.839008656228204 7 82.4181232198782 3.9668123380285665 11.621648847536559 1.993415594556669 8 82.92770564076044 3.655493538466671 11.569612667313002 1.8471881534598926 9 77.82514431859232 5.301594899299404 13.303232338657365 3.570028443450916 10 39.02546693007711 26.208205034099418 20.701801889086582 14.064526146736894 11 32.43470935130581 25.352591639178172 26.874730114447516 15.337968895068496 12 28.74142381511821 22.93409627399134 31.381538127890945 16.942941782999505 13 22.60314360125015 28.287214049640337 31.62933557304924 17.480306776060278 14 19.89495236219235 30.208959590794155 32.67147076317378 17.22461728383972 15 19.299134890483412 22.909265199088118 40.08261625851523 17.708983651913243 16 21.927699866091853 21.2792045843169 38.18974406027214 18.60335148931911 17 21.461748274496948 21.57916653566724 32.503812885338824 24.45527230449699 18 24.00991703084507 22.09208543173026 34.4327446807282 19.465252856696466 19 26.950827413763346 23.48609042746022 29.979512759130234 19.583569399646205 20 28.28464753027049 23.6599079517828 29.619365928557084 18.436078589389624 21 25.261672690501502 25.155097973668795 31.371721191301294 18.211508144528405 22 24.578914375139156 22.877825336807543 30.540233078456573 22.00302720959673 23 21.927828192060346 26.44580056476259 30.523743191505332 21.102628051671736 24 21.16640605801232 24.054831119817315 35.885651295996034 18.893111526174327 25 21.330919949619226 24.529829692190916 34.198998929119796 19.940251429070067 26 20.678446562821016 26.80370169088712 31.195593799545858 21.322257946746006 27 20.619288291346148 26.80504911355629 32.21700434575892 20.35865824933864 28 19.828030369623704 25.776067335202185 34.71557511528484 19.680327179889268 29 20.529331787433165 24.576604507706296 34.74432013222708 20.14974357263346 30 21.355815187506696 25.743472539205186 33.647325590572144 19.253386682715966 31 25.55829816667105 25.19391657913766 30.298851931722886 18.9489333224684 32 26.17362118559079 24.6710524205165 30.199784284046967 18.955542109845748 33 24.990327430124907 25.82624278888261 28.510180419895402 20.673249361097085 34 21.348051466412922 26.53248475647902 30.734582757737893 21.384881019370162 35 21.74470703502208 26.719134877650813 30.89223121003047 20.643926877296632 36 25.279574163106155 28.028893875065684 27.076907677806854 19.6146242840213 37 21.77261793316912 29.945891355385296 28.232098046172965 20.04939266527262 38 22.389801678631994 29.757701322591597 27.178349355899883 20.674147642876527 39 21.83466353893506 29.10503544684065 27.532593191922395 21.5277078223019 40 24.4763177633297 26.14250213823145 28.105953619145208 21.275226479293643 41 20.536967182558445 25.94481598376933 29.00699440691266 24.511222426759556 42 22.752065887685262 26.50784617052853 27.249826920349996 23.49026102143621 43 22.365676396555475 26.879349849313233 27.988471194990666 22.766502559140626 44 22.032606345334163 27.19490340583537 29.40891134023 21.36357890860047 45 20.9769327655337 29.97322478667412 27.54799230814145 21.501850139650735 46 22.052304381497706 29.076226266914162 27.92912043456306 20.94234891702507 47 21.57133865158922 28.76817977954882 28.331037367880395 21.329444200981566 48 21.993466924944066 27.38206683088113 29.925359200426556 20.699107043748246 49 22.367472960114366 26.524400220464013 30.52643803684367 20.581688782577952 50 20.968655740565957 29.479811437821894 29.02906647349331 20.522466348118837 51 20.49718613232589 29.25344442940179 28.001175465871388 22.248193972400934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 884.0 1 1689.0 2 2494.0 3 15836.0 4 29178.0 5 21022.0 6 12866.0 7 12866.0 8 12866.0 9 13074.5 10 13283.0 11 13375.5 12 13468.0 13 13274.0 14 13080.0 15 12426.5 16 11773.0 17 11128.5 18 10484.0 19 10147.0 20 9810.0 21 9789.5 22 9769.0 23 10056.5 24 10344.0 25 11055.5 26 13305.0 27 14843.0 28 17545.0 29 20247.0 30 22611.5 31 24976.0 32 29368.5 33 33761.0 34 39642.5 35 45524.0 36 48599.0 37 51674.0 38 56353.5 39 61033.0 40 69638.5 41 78244.0 42 89625.0 43 101006.0 44 103579.5 45 106153.0 46 106416.0 47 106679.0 48 104311.0 49 101943.0 50 103129.5 51 104316.0 52 100235.0 53 96154.0 54 92319.0 55 88484.0 56 84607.5 57 80731.0 58 76038.0 59 71345.0 60 65221.5 61 59098.0 62 52894.5 63 46691.0 64 40851.5 65 35012.0 66 29617.5 67 24223.0 68 20373.5 69 16524.0 70 13719.5 71 10915.0 72 9012.5 73 7110.0 74 5767.0 75 3477.0 76 2530.0 77 1962.0 78 1394.0 79 1111.5 80 829.0 81 590.0 82 351.0 83 251.0 84 151.0 85 104.5 86 58.0 87 40.5 88 23.0 89 18.5 90 14.0 91 8.0 92 2.0 93 2.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1558531.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.875342161142942 #Duplication Level Percentage of deduplicated Percentage of total 1 75.40090687524174 18.756233577821142 2 5.952645553194959 2.9614818979946125 3 2.694149612060759 2.010536802999657 4 1.7918114664136846 1.7828769326119875 5 1.3148253572398834 1.6353365321744553 6 1.0799391599443735 1.6118313670100142 7 0.8652378819097478 1.5066161854301297 8 0.7620825740121971 1.5165651826878357 9 0.6493013180435573 1.4536433206813126 >10 8.488983088509368 48.53792437941715 >50 0.9435830126202095 14.606105311660981 >100 0.05471878570541183 2.174483112855709 >500 0.001037322916694392 0.16107998730866588 >1k 5.18661458347196E-4 0.2264925252065382 >5k 0.0 0.0 >10k+ 2.59330729173598E-4 1.0587928841398209 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16413 1.0531070604306236 No Hit GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA 1763 0.11311934122580816 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1748 0.11215689646211721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008597839888972373 0.0 2 0.0 0.0 0.0 0.02611433458814743 0.0 3 0.0 0.0 0.0 0.041770102744186674 0.0 4 0.0 0.0 0.0 0.0601848792228066 0.0 5 0.0 0.0 0.0 0.10535562013203459 0.0 6 0.0 0.0 0.0 0.18305699405401624 0.0 7 0.0 0.0 0.0 0.23368158862415955 0.0 8 0.0 0.0 0.0 0.35392302110128065 0.0 9 0.0 0.0 0.0 0.4208450136699238 0.0 10 0.0 0.0 0.0 0.5194635204561219 0.0 11 0.0 0.0 0.0 0.5845247864816292 0.0 12 0.0 0.0 0.0 0.6426564502085618 0.0 13 0.0 0.0 0.0 0.6764703429062368 0.0 14 0.0 0.0 0.0 0.6943076525266421 0.0 15 0.0 0.0 0.0 0.7145189925641517 0.0 16 0.0 0.0 0.0 0.7461513437974605 0.0 17 0.0 0.0 0.0 0.781505148117041 0.0 18 0.0 0.0 0.0 0.8286649415378969 0.0 19 0.0 0.0 0.0 0.8537526683781074 0.0 20 6.416298424606247E-5 0.0 0.0 0.8807652847456996 0.0 21 6.416298424606247E-5 0.0 0.0 0.9146433404276206 0.0 22 6.416298424606247E-5 0.0 0.0 0.9527561530697818 0.0 23 1.2832596849212495E-4 0.0 0.0 0.9967719602625806 0.0 24 1.2832596849212495E-4 0.0 0.0 1.0360397066211708 0.0 25 1.2832596849212495E-4 0.0 0.0 1.0694686214133693 0.0 26 1.2832596849212495E-4 0.0 0.0 1.101999254426123 0.0 27 1.2832596849212495E-4 0.0 0.0 1.1413311637689594 0.0 28 1.9248895273818743E-4 0.0 0.0 1.1782890426946913 0.0 29 1.9248895273818743E-4 0.0 0.0 1.2167868332423288 0.0 30 1.9248895273818743E-4 0.0 0.0 1.276522571575413 0.0 31 1.9248895273818743E-4 0.0 0.0 1.3239390169332532 0.0 32 1.9248895273818743E-4 0.0 0.0 1.3820065176759397 0.0 33 1.9248895273818743E-4 0.0 0.0 1.4335293940255278 0.0 34 1.9248895273818743E-4 0.0 0.0 1.485886389170315 0.0 35 1.9248895273818743E-4 0.0 0.0 1.5410665556219285 0.0 36 1.9248895273818743E-4 0.0 0.0 1.5921402910817943 0.0 37 1.9248895273818743E-4 0.0 0.0 1.646293849785471 0.0 38 1.9248895273818743E-4 0.0 0.0 1.7093019003151044 0.0 39 1.9248895273818743E-4 0.0 0.0 1.8088828518649933 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTACG 20 7.034061E-4 45.0 13 TTAACGC 20 7.034061E-4 45.0 30 CGTTTTT 12725 0.0 42.117878 1 TCGTAAG 95 0.0 40.263157 1 ACGGGAC 265 0.0 39.056602 5 GGGCGAT 3330 0.0 38.91892 7 GGCGATA 575 0.0 38.739132 8 CGCGTCA 35 6.24972E-6 38.57143 39 GGTACCT 855 0.0 37.894737 8 ACGGGTA 180 0.0 37.5 5 GGGTACC 1030 0.0 37.35437 7 GCGCGAC 465 0.0 36.774193 9 AGGGCGA 1875 0.0 36.72 6 GACACGA 480 0.0 36.562504 25 ATAGGGC 900 0.0 36.5 4 AACACGT 475 0.0 36.473682 41 TAGCCGT 155 0.0 36.29032 44 TACGGGT 180 0.0 36.25 4 GTTTTTT 15540 0.0 36.153477 2 GACCGAT 855 0.0 36.05263 9 >>END_MODULE