FastQCFastQC Report
Thu 26 May 2016
SRR1545470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545470_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences826864
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49600.5998568083747751No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32130.38857659784438553No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11960.14464289169682076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT207.032136E-445.00000414
CTATGCG207.032136E-445.0000041
ACACGCG207.032136E-445.00000436
CGAACAC207.032136E-445.00000415
TTGTGCG207.032136E-445.0000041
CCCTAGT207.032136E-445.00000426
TTCCGCG207.032136E-445.0000041
CGTTAGA207.032136E-445.00000413
ACGATAT207.032136E-445.00000430
CCGTAAG302.1647593E-645.0000041
CATAGAC207.032136E-445.00000417
GTCGTGA207.032136E-445.00000430
TCCGTCG207.032136E-445.00000426
TAGATTA207.032136E-445.00000444
ACGTCAC207.032136E-445.00000442
CATCGAA207.032136E-445.0000049
CACGTCG207.032136E-445.00000428
TCGGTAC406.8102963E-945.00000436
TACAAGT302.1647593E-645.00000445
GGTAATC253.889852E-545.08