##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545470_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	826864
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.904955349368215	31.0	31.0	33.0	28.0	34.0
2	31.185020753110546	31.0	31.0	34.0	28.0	34.0
3	31.27569346349581	31.0	31.0	34.0	28.0	34.0
4	35.111613276185686	35.0	35.0	37.0	32.0	37.0
5	34.962199345962574	35.0	35.0	37.0	32.0	37.0
6	35.02211367286519	37.0	35.0	37.0	32.0	37.0
7	35.541706979624216	37.0	35.0	37.0	35.0	37.0
8	35.476493111321815	37.0	35.0	37.0	35.0	37.0
9	37.160023897521235	39.0	37.0	39.0	34.0	39.0
10	36.580463050755625	39.0	35.0	39.0	32.0	39.0
11	36.23560827415391	38.0	35.0	39.0	32.0	39.0
12	35.16471995394648	37.0	34.0	39.0	30.0	39.0
13	34.54585518295632	37.0	33.0	39.0	26.0	39.0
14	35.46829466514445	37.0	33.0	40.0	27.0	41.0
15	35.92562622148262	37.0	34.0	40.0	30.0	41.0
16	36.12477142553068	37.0	34.0	40.0	31.0	41.0
17	36.05141595232106	37.0	34.0	40.0	31.0	41.0
18	36.02136868940963	37.0	34.0	40.0	30.0	41.0
19	35.90171176880357	37.0	34.0	40.0	30.0	41.0
20	35.720082141682305	36.0	34.0	40.0	30.0	41.0
21	35.528084425008224	36.0	34.0	40.0	29.0	41.0
22	35.42251567367789	35.0	34.0	40.0	29.0	41.0
23	35.422543489618604	35.0	34.0	40.0	29.0	41.0
24	35.30582538361811	35.0	34.0	40.0	29.0	41.0
25	35.166648928965344	35.0	34.0	40.0	29.0	41.0
26	34.98633027922367	35.0	34.0	40.0	29.0	41.0
27	34.84799797790205	35.0	34.0	40.0	27.0	41.0
28	34.915740678031696	36.0	34.0	40.0	28.0	41.0
29	34.99443560246909	36.0	34.0	40.0	29.0	41.0
30	34.92559961493063	36.0	34.0	40.0	29.0	41.0
31	34.720821078194234	35.0	34.0	40.0	27.0	41.0
32	34.468736092029644	35.0	33.0	40.0	26.0	41.0
33	34.20905009771861	35.0	33.0	40.0	24.0	41.0
34	34.06086127827551	35.0	33.0	40.0	24.0	41.0
35	33.87511852009521	35.0	33.0	40.0	23.0	41.0
36	33.63617596122216	35.0	33.0	40.0	23.0	41.0
37	33.608477331217706	35.0	33.0	40.0	23.0	41.0
38	33.61871117862188	35.0	33.0	40.0	23.0	41.0
39	33.587724220669905	35.0	33.0	40.0	23.0	41.0
40	33.423888329882544	35.0	32.0	39.0	23.0	41.0
41	33.41431722750053	35.0	32.0	39.0	23.0	41.0
42	33.36595619110277	35.0	32.0	39.0	23.0	41.0
43	33.2427605990828	35.0	32.0	39.0	23.0	41.0
44	33.17308408637938	35.0	32.0	39.0	22.0	41.0
45	33.136536359062674	35.0	32.0	39.0	23.0	41.0
46	33.08009418719403	35.0	32.0	39.0	23.0	41.0
47	33.008397995317246	35.0	32.0	39.0	22.0	41.0
48	33.00534428878268	35.0	32.0	39.0	22.0	41.0
49	33.05104346059328	35.0	32.0	39.0	22.0	40.0
50	32.92545086011726	35.0	32.0	39.0	22.0	40.0
51	32.708811848139476	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	2.0
14	14.0
15	40.0
16	145.0
17	311.0
18	661.0
19	1390.0
20	2306.0
21	3613.0
22	5331.0
23	7470.0
24	10292.0
25	13739.0
26	17951.0
27	20746.0
28	22696.0
29	24986.0
30	28822.0
31	35174.0
32	42850.0
33	54373.0
34	90450.0
35	121745.0
36	56227.0
37	64746.0
38	85704.0
39	115037.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.63668511387604	23.321489386404537	27.065273128350007	16.976552371369415
2	30.732260686158785	27.310899978714758	29.28002670330308	12.67681263182337
3	28.70871146887517	25.211763966021017	32.88811703012829	13.191407534975522
4	26.69701426111186	27.30509491282726	31.459587066313205	14.538303759747674
5	22.237756148532288	32.20940807678167	31.423789160006965	14.129046614679078
6	20.66155982120397	40.229711294723195	28.59696395054084	10.511764933531996
7	84.18990305539968	5.508765649490122	8.523287989318678	1.7780433057915206
8	86.45242748505196	3.670615723988467	8.123585015189922	1.7533717757696548
9	81.47942104142882	5.555568993208073	9.879012751794733	3.085997213568374
10	54.95413997948877	20.11673019988777	14.852019195417867	10.077110625205595
11	49.28271154627605	19.676391764546526	19.061901352580353	11.97899533659707
12	44.060691963853785	20.64341899030554	23.26464811625612	12.03124092958455
13	21.39505408386385	42.500822384334064	22.737596509220378	13.366527022581707
14	15.069588227326381	43.65905396776253	28.301994040132357	12.96936376477873
15	13.95054083863852	21.63826215677548	51.52794171713849	12.883255287447511
16	15.877459896669826	16.55677354437973	48.75033862884344	18.815427930107006
17	15.184842972967743	16.952485535710828	31.327159000754655	36.53551249056677
18	21.711309235859826	21.26250507943265	37.03837632307126	19.987809361636256
19	31.525135935292862	21.920412546682407	26.76691692950715	19.78753458851758
20	33.33583273670156	21.690265872017648	25.848023375065303	19.125878016215484
21	22.036271986687048	28.15251359352929	28.580516263859597	21.23069815592407
22	22.305240039474448	24.886922154066447	25.920586698659033	26.887251107800076
23	19.57335184504344	29.697870508330272	26.14529112405426	24.58348652257203
24	19.25117068828731	22.569491476228254	42.25531647284197	15.924021362642465
25	17.01368060527487	24.598724820526712	38.757280520133904	19.630314054064513
26	16.37161613034308	34.72590413901197	28.338517579674527	20.563962150970415
27	18.04129820623464	35.07759438069622	28.745476886162656	18.13563052690648
28	14.882616730199889	28.966674084250855	39.63118481394764	16.51952437160162
29	15.020003289537337	24.73272509142979	39.258451208421214	20.988820410611662
30	17.653689119371503	31.342881054199967	32.533040500009676	18.470389326418854
31	30.043997561872327	27.34403722982256	25.077763695117937	17.534201513187174
32	32.18497842450512	25.82359372278875	26.50714023104162	15.484287621664505
33	29.37641498481008	27.0710781942375	24.62303353393061	18.929473287021807
34	19.046904932370982	27.61796377638886	28.729150138354072	24.605981152886088
35	20.71441010855473	25.269814624895993	32.10527970742468	21.910495559124595
36	30.79442326670408	26.46795603630101	25.528502873507612	17.209117823487297
37	20.919280558834345	35.13431471197198	27.83432341957081	16.112081309622862
38	20.454996226707173	35.52458445403355	23.780815224752804	20.239604094506472
39	21.249927436676405	33.53743783741945	26.053740397453513	19.15889432845063
40	24.674190677064185	26.53181176106349	24.969644342963292	23.82435321890903
41	17.041012790495174	25.195316279339767	26.883260125002415	30.88041080516264
42	20.94903152150777	25.989400917200413	23.778517386172332	29.283050175119484
43	21.452016292884924	26.019514696491807	26.404100311538535	26.124368699084734
44	19.87533621006598	28.89459451614776	29.614785502815455	21.6152837709708
45	16.850292188316338	38.920185181601816	23.049135045182762	21.180387584899087
46	20.98664351090385	35.416706979624216	24.86515315698833	18.7314963524836
47	21.36772189864355	28.469857195379166	26.67318930319859	23.48923160277869
48	22.56840302637435	23.85543450918168	32.10721472938718	21.468947735056794
49	21.053401768610076	24.088846533408155	33.784153137638114	21.073598560343658
50	19.95164863871205	35.12004392499855	26.20987248205267	18.71843495423673
51	17.86666247411908	35.53377580835542	24.455291317556455	22.14427039996904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	606.0
1	1035.0
2	1464.0
3	5058.0
4	8652.0
5	6489.5
6	4327.0
7	4503.0
8	4679.0
9	4901.0
10	5123.0
11	5306.0
12	5489.0
13	5252.0
14	5015.0
15	4701.0
16	4387.0
17	4116.5
18	3846.0
19	3855.0
20	3864.0
21	3529.5
22	3195.0
23	3428.5
24	3662.0
25	4205.0
26	5331.5
27	5915.0
28	7703.5
29	9492.0
30	10681.0
31	11870.0
32	13608.5
33	15347.0
34	18175.0
35	21003.0
36	22089.5
37	23176.0
38	28337.5
39	33499.0
40	46583.0
41	59667.0
42	69733.0
43	79799.0
44	84931.0
45	90063.0
46	82624.0
47	75185.0
48	68639.5
49	62094.0
50	58400.5
51	54707.0
52	52434.0
53	50161.0
54	45937.0
55	41713.0
56	37858.0
57	34003.0
58	32091.5
59	30180.0
60	28003.0
61	25826.0
62	21589.0
63	17352.0
64	14237.5
65	11123.0
66	8895.5
67	6668.0
68	5226.0
69	3784.0
70	3035.5
71	2287.0
72	1789.0
73	1291.0
74	1037.5
75	621.0
76	458.0
77	327.5
78	197.0
79	135.0
80	73.0
81	59.0
82	45.0
83	36.5
84	28.0
85	19.5
86	11.0
87	6.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	826864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.329946213406835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67236707351374	15.593925164392331
2	5.444859105538007	2.1049766729925623
3	1.8698187432220867	1.0843048720590873
4	0.9753587800362105	0.7541453102749621
5	0.704820337249912	0.6812069604578033
6	0.5488837816381612	0.6365936385886198
7	0.4610195217243204	0.6238037790783161
8	0.38300115798086315	0.5922713426754095
9	0.36011439672231443	0.6264892727384209
>10	5.5162931332856395	26.330284328855
>50	2.403833525080667	33.1333475901396
>100	0.6546237613211086	16.30337468108589
>500	0.003129176679355203	0.4018744115113924
>1k	0.0018775060076131219	1.1334019751505826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4960	0.5998568083747751	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3213	0.38857659784438553	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1196	0.14464289169682076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007981965595309507	0.0
2	0.0	0.0	0.0	0.024550591149209544	0.0
3	0.0	0.0	0.0	0.033379128853112484	0.0
4	0.0	0.0	0.0	0.059380986474196484	0.0
5	0.0	0.0	0.0	0.09941175332340021	0.0
6	0.0	0.0	0.0	0.15129452969291202	0.0
7	0.0	0.0	0.0	0.18165018672961938	0.0
8	0.0	0.0	0.0	0.28807639466707946	0.0
9	0.0	0.0	0.0	0.32883279475222044	0.0
10	0.0	0.0	0.0	0.4005495462373498	0.0
11	0.0	0.0	0.0	0.4521904448615492	0.0
12	0.0	0.0	0.0	0.5154414752607442	0.0
13	0.0	0.0	0.0	0.5402339441552662	0.0
14	0.0	0.0	0.0	0.5488206041138567	0.0
15	0.0	0.0	0.0	0.5621238801060392	0.0
16	0.0	0.0	0.0	0.5905445151802473	0.0
17	0.0	0.0	0.0	0.6198117223630488	0.0
18	0.0	0.0	0.0	0.67012229338803	0.0
19	0.0	0.0	0.0	0.6901981462489599	0.0
20	0.0	0.0	0.0	0.7169256371059811	0.0
21	0.0	0.0	0.0	0.7482488051239382	0.0
22	0.0	0.0	0.0	0.7787254010333017	0.0
23	0.0	0.0	0.0	0.8189980456278179	0.0
24	0.0	0.0	0.0	0.8464511697207764	0.0
25	0.0	0.0	0.0	0.8714855163606107	0.0
26	0.0	0.0	0.0	0.900631784670756	0.0
27	0.0	0.0	0.0	0.9249404980746532	0.0
28	0.0	0.0	0.0	0.9493701503512065	0.0
29	0.0	0.0	0.0	0.9783954797886956	0.0
30	0.0	0.0	0.0	1.0220544128175855	0.0
31	1.2093887265620465E-4	0.0	0.0	1.0594245244683527	0.0
32	1.2093887265620465E-4	0.0	0.0	1.0946177364113083	0.0
33	1.2093887265620465E-4	0.0	0.0	1.132350664680044	0.0
34	1.2093887265620465E-4	0.0	0.0	1.1709301650573734	0.0
35	2.418777453124093E-4	0.0	0.0	1.2242642078987596	0.0
36	2.418777453124093E-4	0.0	0.0	1.2678022020549933	0.0
37	2.418777453124093E-4	0.0	0.0	1.3096470519940402	0.0
38	2.418777453124093E-4	0.0	0.0	1.3554828847307416	0.0
39	2.418777453124093E-4	0.0	0.0	1.40301186168463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.032136E-4	45.000004	14
CTATGCG	20	7.032136E-4	45.000004	1
ACACGCG	20	7.032136E-4	45.000004	36
CGAACAC	20	7.032136E-4	45.000004	15
TTGTGCG	20	7.032136E-4	45.000004	1
CCCTAGT	20	7.032136E-4	45.000004	26
TTCCGCG	20	7.032136E-4	45.000004	1
CGTTAGA	20	7.032136E-4	45.000004	13
ACGATAT	20	7.032136E-4	45.000004	30
CCGTAAG	30	2.1647593E-6	45.000004	1
CATAGAC	20	7.032136E-4	45.000004	17
GTCGTGA	20	7.032136E-4	45.000004	30
TCCGTCG	20	7.032136E-4	45.000004	26
TAGATTA	20	7.032136E-4	45.000004	44
ACGTCAC	20	7.032136E-4	45.000004	42
CATCGAA	20	7.032136E-4	45.000004	9
CACGTCG	20	7.032136E-4	45.000004	28
TCGGTAC	40	6.8102963E-9	45.000004	36
TACAAGT	30	2.1647593E-6	45.000004	45
GGTAATC	25	3.889852E-5	45.0	8
>>END_MODULE