FastQCFastQC Report
Thu 26 May 2016
SRR1545468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545468_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1290741
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70620.5471275802039294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49090.3803241703796501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG453.8562575E-1045.0000041
CACGCAT351.2120836E-745.00000442
CATTACG351.2120836E-745.00000429
TCGAATA351.2120836E-745.00000425
TAACTCG351.2120836E-745.00000438
CTTACGT453.8562575E-1045.00000422
TCGCCTA207.0336094E-445.027
TAATACG253.891073E-545.01
GGTACGA253.891073E-545.07
TCACCGT253.891073E-545.015
ACGCACC253.891073E-545.020
CACGCTA207.0336094E-445.030
ATTCGGT207.0336094E-445.044
TTCGTCA207.0336094E-445.027
TTCGTAG406.8157533E-945.01
TTGTACG253.891073E-545.01
GTCTACG207.0336094E-445.032
TAAGTCC253.891073E-545.043
TAACGGC253.891073E-545.018
ACTTACG207.0336094E-445.01