##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545468_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1290741
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.029668229334934	31.0	31.0	33.0	30.0	34.0
2	31.324986190103203	31.0	31.0	34.0	30.0	34.0
3	31.388795273412715	31.0	31.0	34.0	28.0	34.0
4	35.210465926161795	37.0	35.0	37.0	33.0	37.0
5	35.08395797452781	37.0	35.0	37.0	32.0	37.0
6	35.133464420824936	37.0	35.0	37.0	32.0	37.0
7	35.6452053510348	37.0	35.0	37.0	35.0	37.0
8	35.59398283621579	37.0	35.0	37.0	35.0	37.0
9	37.310692850076045	39.0	37.0	39.0	35.0	39.0
10	36.75994176988257	39.0	37.0	39.0	32.0	39.0
11	36.41058508252237	38.0	35.0	39.0	32.0	39.0
12	35.19710538365172	37.0	35.0	39.0	30.0	39.0
13	34.50432038650666	35.0	33.0	39.0	26.0	39.0
14	35.37191737149436	37.0	33.0	40.0	27.0	41.0
15	35.85132261235988	37.0	34.0	40.0	30.0	41.0
16	36.08166161917844	37.0	34.0	40.0	31.0	41.0
17	36.017714630588166	36.0	34.0	40.0	31.0	41.0
18	35.962071399296995	36.0	34.0	40.0	31.0	41.0
19	35.83723070701248	36.0	34.0	40.0	30.0	41.0
20	35.66008672537713	36.0	34.0	40.0	30.0	41.0
21	35.457512390169676	35.0	34.0	40.0	30.0	41.0
22	35.35240919750748	35.0	34.0	40.0	30.0	41.0
23	35.33514856969756	35.0	34.0	40.0	30.0	41.0
24	35.24659478547594	35.0	34.0	40.0	29.0	41.0
25	35.12658310226451	35.0	34.0	40.0	29.0	41.0
26	34.975855729383355	35.0	34.0	40.0	29.0	41.0
27	34.88264260606892	35.0	34.0	40.0	29.0	41.0
28	34.94240052806876	35.0	34.0	40.0	29.0	41.0
29	35.06208759154625	35.0	34.0	40.0	29.0	41.0
30	34.94170790266986	35.0	34.0	40.0	29.0	41.0
31	34.69860645939038	35.0	34.0	40.0	28.0	41.0
32	34.40615274481868	35.0	33.0	40.0	27.0	41.0
33	34.14658014272422	35.0	33.0	40.0	25.0	41.0
34	33.950914242284085	35.0	33.0	40.0	23.0	41.0
35	33.7482500362195	35.0	33.0	39.0	23.0	41.0
36	33.52444681001069	35.0	33.0	39.0	23.0	41.0
37	33.44614682573808	35.0	33.0	39.0	23.0	41.0
38	33.48111201240218	35.0	33.0	39.0	23.0	41.0
39	33.47006641921191	35.0	33.0	39.0	23.0	41.0
40	33.26613085041848	35.0	32.0	39.0	22.0	41.0
41	33.26873323153134	35.0	32.0	39.0	22.0	41.0
42	33.239306723812135	35.0	32.0	39.0	23.0	41.0
43	33.113401526719926	35.0	32.0	39.0	22.0	41.0
44	33.04562495496773	35.0	32.0	39.0	22.0	41.0
45	32.9877217815193	35.0	32.0	39.0	22.0	41.0
46	32.953040152904414	35.0	32.0	38.0	22.0	40.0
47	32.89590243123911	35.0	32.0	38.0	22.0	40.0
48	32.90141012023326	35.0	32.0	38.0	22.0	40.0
49	32.942884746048975	35.0	32.0	38.0	22.0	40.0
50	32.79843748668401	35.0	32.0	38.0	21.0	40.0
51	32.62446842550132	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	8.0
14	18.0
15	52.0
16	133.0
17	422.0
18	997.0
19	1896.0
20	3284.0
21	5186.0
22	7569.0
23	10994.0
24	15653.0
25	22105.0
26	28357.0
27	32923.0
28	34745.0
29	37881.0
30	44750.0
31	54470.0
32	67567.0
33	87546.0
34	150730.0
35	204448.0
36	83042.0
37	95996.0
38	127443.0
39	172458.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.222220414475093	23.371691144854005	28.47798280212684	16.92810563854406
2	31.97558611681197	25.317860050931984	29.208880790181762	13.497673042074283
3	27.889793537200724	24.512818605746624	33.63323858155896	13.96414927549369
4	25.65913688338714	27.814178057410434	31.810409679401214	14.716275379801214
5	23.1109107094297	31.10647294848463	31.8304756725013	13.95214066958437
6	20.72328995514979	39.020221717602524	29.6408807034099	10.61560762383778
7	84.07767321251902	4.97040072330545	9.185498872353168	1.7664271918223717
8	86.02988515899007	3.384335044753363	8.745364097057427	1.840415699199142
9	81.8869161202751	4.645858464246507	10.632109772603489	2.8351156428749067
10	57.07705883674571	18.402762444208403	14.75338584580485	9.76679287324103
11	53.47261766690606	17.996949039350266	18.170647713212798	10.359785580530874
12	46.42782711636184	20.352107820236593	22.532560753861542	10.687504309540023
13	21.700325626907336	43.81134557591337	22.34344457950898	12.144884217670315
14	14.177979935556397	46.05951155189151	27.889406162816556	11.87310234973554
15	12.754224123972199	21.793605378615847	54.038571642180735	11.41359885523122
16	14.943199294048922	15.695945197371122	51.84510292924762	17.515752579332336
17	14.491288337474364	15.717018363870055	31.459913336602774	38.331779962052806
18	21.550256790479267	20.90295419452857	37.64341568137992	19.903373333612244
19	30.861574862811363	21.81816491457233	27.303153769811296	20.017106452805017
20	33.885341830777826	21.233307069350086	26.13243090596797	18.748920193904123
21	22.825803162679424	26.974892716664307	30.21241286981664	19.986891250839633
22	22.969751483837577	25.005868721920198	26.366095134500263	25.658284659741966
23	19.341680476563464	29.945744343752928	25.92596035920452	24.786614820479087
24	19.15419127462442	21.642064519527928	43.105859347460104	16.097884858387545
25	16.598295087860386	23.075582165593254	40.44304783066471	19.88307491588165
26	15.23977312257068	33.92423421894865	30.346831781124177	20.489160877356497
27	17.03455611931441	34.17176645043429	30.541448671731974	18.252228758519333
28	13.229687443104387	29.660714271879485	41.49112796447932	15.61847032053681
29	14.756872215262396	24.764611955458143	39.75685284654319	20.721662982736273
30	17.640719555666088	30.16042722746082	34.03541066720589	18.163442549667206
31	29.63925373099638	27.1035784870861	26.022494055740076	17.234673726177444
32	32.52232632263173	24.46788317718272	28.688636992239342	14.32115350794621
33	29.511497659096598	26.92546374524401	25.446390871600112	18.11664772405928
34	19.90871910011381	27.658685979603963	29.45463109950021	22.977963820782016
35	19.88857563213689	25.02670946378863	32.45763480047508	22.627080103599408
36	32.50210537977797	24.88973388154556	25.691753806534383	16.91640693214208
37	21.2211435136871	33.12996178164326	29.48120498225438	16.16768972241526
38	20.538899748284127	33.74356280617103	24.212990832397825	21.50454661314702
39	20.597238330540364	32.1935229453469	27.689443505707185	19.51979521840555
40	24.136368179208688	24.165731157528892	26.096095188732676	25.601805474529748
41	16.152814546063077	24.534976420521236	28.426539483908858	30.885669549506833
42	21.668328502774763	25.158571704160632	25.53014121345801	27.642958579606596
43	22.13224806525864	25.721736583869266	27.235440727458105	24.910574623413993
44	19.785843945454587	29.780025582204328	28.463727424789326	21.970403047551756
45	16.810188876002236	38.46155038075028	23.057607994167693	21.670652749079792
46	21.162572506800355	34.10335613418958	25.356984863733313	19.377086495276743
47	21.626801968791572	28.868921030632794	27.049113648671575	22.455163351904062
48	23.011045593190268	23.795711145768205	31.259020980971396	21.93422228007013
49	22.05004722093743	23.970416993029588	32.34374673152863	21.63578905450435
50	19.635155310011847	35.34853235467069	25.133082469682144	19.88322986563532
51	17.886159965477194	34.35584675779262	24.49747858013343	23.26051469659676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	935.0
1	1497.5
2	2060.0
3	7639.0
4	13218.0
5	10015.0
6	6812.0
7	7304.0
8	7796.0
9	8584.5
10	9373.0
11	9764.0
12	10155.0
13	9599.5
14	9044.0
15	8834.0
16	8624.0
17	7994.0
18	7364.0
19	7137.5
20	6911.0
21	6739.0
22	6567.0
23	7186.5
24	7806.0
25	8063.5
26	8029.0
27	7737.0
28	9647.5
29	11558.0
30	12205.5
31	12853.0
32	18117.0
33	23381.0
34	24359.5
35	25338.0
36	27902.0
37	30466.0
38	39777.0
39	49088.0
40	70605.5
41	92123.0
42	113761.0
43	135399.0
44	134389.5
45	133380.0
46	129024.0
47	124668.0
48	110039.5
49	95411.0
50	94783.5
51	94156.0
52	87918.5
53	81681.0
54	70979.5
55	60278.0
56	56350.5
57	52423.0
58	51044.5
59	49666.0
60	47393.5
61	45121.0
62	36093.0
63	27065.0
64	21355.0
65	15645.0
66	11952.5
67	8260.0
68	6430.5
69	4601.0
70	3417.5
71	2234.0
72	1837.5
73	1441.0
74	1140.0
75	641.0
76	443.0
77	307.5
78	172.0
79	156.5
80	141.0
81	117.5
82	94.0
83	68.0
84	42.0
85	36.0
86	30.0
87	18.5
88	7.0
89	8.0
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1290741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.39858638782545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.18439592082782	14.472908985475977
2	5.92002211166372	2.0600003225523618
3	1.8405354565191754	0.9606814542031389
4	0.8902814356930658	0.6195855387353231
5	0.5476865754151835	0.47644860979066733
6	0.3916421691635448	0.4088412067984369
7	0.339085272203884	0.41297230808950086
8	0.26306596632411805	0.3661580752629559
9	0.2320514226976509	0.36336300518005904
>10	3.264807334080781	14.207116675980663
>50	1.4082558332469903	17.889324672780816
>100	1.7132512217049718	46.38314552874563
>500	0.004024865830160466	0.4480170299090247
>1k	4.47207314462274E-4	0.38195824936148004
>5k	4.47207314462274E-4	0.5494783371339931
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7062	0.5471275802039294	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4909	0.3803241703796501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014797701475354079	0.0
2	0.0	0.0	0.0	0.05717645910372414	0.0
3	0.0	0.0	0.0	0.08026397240034988	0.0
4	0.0	0.0	0.0	0.11512766697579142	0.0
5	0.0	0.0	0.0	0.19206021967226578	0.0
6	0.0	0.0	0.0	0.273641264978799	0.0
7	0.0	0.0	0.0	0.32446478418210933	0.0
8	0.0	0.0	0.0	0.45113620780621366	0.0
9	0.0	0.0	0.0	0.5061433703585769	0.0
10	0.0	0.0	0.0	0.6004302954659377	0.0
11	0.0	0.0	0.0	0.699985512198032	0.0
12	0.0	0.0	0.0	0.7848205023316064	0.0
13	0.0	0.0	0.0	0.8204589456753911	0.0
14	0.0	0.0	0.0	0.8357989712885854	0.0
15	0.0	0.0	0.0	0.8607458816292347	0.0
16	0.0	0.0	0.0	0.9162178934426038	0.0
17	7.747487683431456E-5	0.0	0.0	0.9726970786548192	0.0
18	7.747487683431456E-5	0.0	0.0	1.0691533003135407	0.0
19	7.747487683431456E-5	0.0	0.0	1.1092852865137157	0.0
20	7.747487683431456E-5	0.0	0.0	1.1518189938957544	0.0
21	7.747487683431456E-5	0.0	0.0	1.2100026263983248	0.0
22	7.747487683431456E-5	0.0	0.0	1.266249386980037	0.0
23	7.747487683431456E-5	0.0	0.0	1.327609489432814	0.0
24	7.747487683431456E-5	0.0	0.0	1.3768835110994382	0.0
25	7.747487683431456E-5	0.0	0.0	1.414768725871418	0.0
26	7.747487683431456E-5	0.0	0.0	1.4570700086229538	0.0
27	7.747487683431456E-5	0.0	0.0	1.493793100242419	0.0
28	7.747487683431456E-5	0.0	0.0	1.5340800361962625	0.0
29	7.747487683431456E-5	0.0	0.0	1.5765362687014668	0.0
30	7.747487683431456E-5	0.0	0.0	1.6408404164739478	0.0
31	1.5494975366862912E-4	0.0	0.0	1.7011158706510445	0.0
32	1.5494975366862912E-4	0.0	0.0	1.752714138622698	0.0
33	1.5494975366862912E-4	0.0	0.0	1.8064817031457123	0.0
34	1.5494975366862912E-4	0.0	0.0	1.8602492676687268	0.0
35	1.5494975366862912E-4	0.0	0.0	1.9354773730748462	0.0
36	1.5494975366862912E-4	0.0	0.0	1.9990067720789841	0.0
37	1.5494975366862912E-4	0.0	0.0	2.0638532439893056	0.0
38	1.5494975366862912E-4	0.0	0.0	2.1284672912691236	0.0
39	1.5494975366862912E-4	0.0	0.0	2.2015261001238824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	45	3.8562575E-10	45.000004	1
CACGCAT	35	1.2120836E-7	45.000004	42
CATTACG	35	1.2120836E-7	45.000004	29
TCGAATA	35	1.2120836E-7	45.000004	25
TAACTCG	35	1.2120836E-7	45.000004	38
CTTACGT	45	3.8562575E-10	45.000004	22
TCGCCTA	20	7.0336094E-4	45.0	27
TAATACG	25	3.891073E-5	45.0	1
GGTACGA	25	3.891073E-5	45.0	7
TCACCGT	25	3.891073E-5	45.0	15
ACGCACC	25	3.891073E-5	45.0	20
CACGCTA	20	7.0336094E-4	45.0	30
ATTCGGT	20	7.0336094E-4	45.0	44
TTCGTCA	20	7.0336094E-4	45.0	27
TTCGTAG	40	6.8157533E-9	45.0	1
TTGTACG	25	3.891073E-5	45.0	1
GTCTACG	20	7.0336094E-4	45.0	32
TAAGTCC	25	3.891073E-5	45.0	43
TAACGGC	25	3.891073E-5	45.0	18
ACTTACG	20	7.0336094E-4	45.0	1
>>END_MODULE