##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545467_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1090137
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.916925120420643	31.0	31.0	33.0	28.0	34.0
2	31.20240942193504	31.0	31.0	34.0	28.0	34.0
3	31.29321268794656	31.0	31.0	34.0	28.0	34.0
4	35.11609091334392	35.0	35.0	37.0	32.0	37.0
5	34.980746456638016	35.0	35.0	37.0	32.0	37.0
6	35.03889878061198	37.0	35.0	37.0	32.0	37.0
7	35.5639511364168	37.0	35.0	37.0	35.0	37.0
8	35.48519956665997	37.0	35.0	37.0	35.0	37.0
9	37.197169713531416	39.0	37.0	39.0	34.0	39.0
10	36.54624602228894	39.0	35.0	39.0	32.0	39.0
11	36.233870605254204	38.0	35.0	39.0	31.0	39.0
12	35.38743662493797	37.0	35.0	39.0	30.0	39.0
13	34.88101495500107	37.0	34.0	39.0	27.0	39.0
14	35.846945842586756	38.0	34.0	40.0	29.0	41.0
15	36.23007475207245	38.0	35.0	40.0	30.0	41.0
16	36.35489025691266	38.0	35.0	40.0	31.0	41.0
17	36.26763516879071	38.0	34.0	40.0	31.0	41.0
18	36.205134767465005	37.0	35.0	40.0	30.0	41.0
19	36.09859219529289	37.0	35.0	40.0	30.0	41.0
20	35.956708193557326	37.0	34.0	40.0	30.0	41.0
21	35.769900480398334	36.0	34.0	40.0	30.0	41.0
22	35.65268035118522	36.0	34.0	40.0	30.0	41.0
23	35.60067220908932	36.0	34.0	40.0	30.0	41.0
24	35.50565112458342	36.0	34.0	40.0	29.0	41.0
25	35.39090866560809	36.0	34.0	40.0	29.0	41.0
26	35.23449804932775	36.0	34.0	40.0	29.0	41.0
27	35.124989794860646	36.0	34.0	40.0	29.0	41.0
28	35.176765856034606	36.0	34.0	40.0	29.0	41.0
29	35.22902992926577	36.0	34.0	40.0	29.0	41.0
30	35.13568202895599	36.0	34.0	40.0	29.0	41.0
31	34.95893635387112	36.0	34.0	40.0	27.0	41.0
32	34.73215017928939	36.0	34.0	40.0	26.0	41.0
33	34.47268095661371	36.0	33.0	40.0	25.0	41.0
34	34.29330900611575	36.0	33.0	40.0	24.0	41.0
35	34.11735497464998	35.0	33.0	40.0	23.0	41.0
36	33.90120232594619	35.0	33.0	40.0	23.0	41.0
37	33.85926264313568	35.0	33.0	40.0	23.0	41.0
38	33.8616366566771	35.0	33.0	40.0	23.0	41.0
39	33.82286905223839	35.0	33.0	40.0	23.0	41.0
40	33.65794299248626	35.0	33.0	40.0	23.0	41.0
41	33.616409680618126	35.0	33.0	40.0	23.0	41.0
42	33.56867623060221	35.0	33.0	40.0	23.0	41.0
43	33.468875012957085	35.0	32.0	40.0	23.0	41.0
44	33.41878864766539	35.0	32.0	40.0	22.0	41.0
45	33.33863817116564	35.0	32.0	39.0	22.0	41.0
46	33.28308185118017	35.0	32.0	39.0	22.0	41.0
47	33.20761060307099	35.0	32.0	39.0	22.0	41.0
48	33.18173495624862	35.0	32.0	39.0	22.0	41.0
49	33.23351743863386	35.0	32.0	39.0	22.0	41.0
50	33.13603703020813	35.0	32.0	39.0	22.0	41.0
51	32.94822026956245	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	8.0
14	12.0
15	63.0
16	168.0
17	419.0
18	958.0
19	1802.0
20	3047.0
21	4577.0
22	6569.0
23	9480.0
24	12922.0
25	17798.0
26	23085.0
27	26841.0
28	28788.0
29	32039.0
30	37130.0
31	44485.0
32	53688.0
33	67976.0
34	109869.0
35	144990.0
36	78369.0
37	92420.0
38	124490.0
39	168075.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.70071835007894	23.030499836259114	26.812685011149973	16.456096802511976
2	32.32657913638378	25.660444512937364	28.593837288340822	13.419139062338036
3	29.45574730515522	24.394089917138857	32.85614560371769	13.294017173988223
4	26.76168224727718	27.954009450188373	30.910518586196044	14.373789716338406
5	22.430483508036144	31.79416898976918	31.051051381615334	14.72429612057934
6	21.40318143499395	40.25622467634802	28.177375871106108	10.163218017551923
7	84.59689011564602	4.543006979856661	8.939151684604779	1.9209512198925458
8	85.7533502669848	3.999405579298749	8.489208237129828	1.7580359165866306
9	81.28345336411846	5.604433204266987	10.129919450491084	2.9821939811234732
10	49.16877419994001	23.036921047538062	16.165307663165272	11.628997089356659
11	44.1500471959029	21.046253819474067	22.08639831507416	12.717300669548873
12	40.606088959461054	21.10404472098461	25.456616920625574	12.833249398928759
13	21.77038298856015	38.232901002351085	25.15188457964458	14.844831429444188
14	15.57253813052855	40.760473224924944	28.35423437604631	15.312754268500198
15	14.94738734672798	23.78875315671333	46.82631632537929	14.437543171179401
16	17.14545969910204	18.76681554703675	46.50433844553483	17.583386308326386
17	16.854303633396537	19.057971612742254	31.254236852799234	32.83348790106197
18	21.671496334864333	22.584500847141232	35.09357080807275	20.650432009921687
19	29.837167255124815	22.95472954316751	27.61744624758173	19.590656954125947
20	32.509767121013226	21.530596613086246	27.261711142727936	18.697925123172592
21	23.130303805851927	28.189576172536114	28.968285637493267	19.711834384118692
22	22.87244630720726	25.653289448940818	26.76535151086515	24.70891273298677
23	20.132790649248673	30.4347985620156	27.22639448069371	22.206016308042017
24	19.800171904999097	23.527409857660093	39.37734431543925	17.29507392190156
25	17.896374492380314	24.71927840262279	37.10478591222938	20.279561192767513
26	17.62420686574256	33.34727653496763	29.377133332782947	19.65138326650687
27	17.997370972639217	33.93380831950479	29.75332458213968	18.31549612571631
28	16.232455186825142	29.405386662410322	37.64994674981218	16.712211400952356
29	16.556634624822383	25.1360150146266	37.95889874391934	20.34845161663167
30	18.37236971132986	30.4597495544138	32.18742231480997	18.980458419446364
31	27.23290742356236	27.160072541341133	26.87451210260729	18.73250793248922
32	29.876428375516106	26.093784542676744	27.637076807777373	16.392710274029778
33	28.04436506604216	27.450219559559947	26.46584787049701	18.039567503900887
34	20.018493088483375	27.989142649043192	29.42364124876048	22.568723013712955
35	20.91984768886846	26.24981997675522	31.091413281083017	21.7389190532933
36	28.446332892104387	27.228229112487696	26.2260614950231	18.099376500384814
37	20.580073880622344	33.4390998562566	28.739323589603877	17.241502673517182
38	20.39266624286672	33.34791865609552	25.323239189202823	20.936175911834933
39	21.123767012769953	32.27594329886978	26.665822736041434	19.93446695231884
40	24.192647346159244	26.626194689291342	26.873319591941197	22.307838372608213
41	17.42386507383934	26.106993891593444	28.34038290600172	28.12875812856549
42	21.18476851991997	27.08182549532765	25.083728008498014	26.64967797625436
43	21.811753935514528	27.276021270721017	26.520152971598982	24.39207182216547
44	19.740913298053364	30.920975987421766	28.73996571073177	20.5981450037931
45	17.79308472237893	36.675390340847066	24.53150383850837	21.00002109826563
46	20.253050763344422	34.60023831867004	25.93270387116482	19.214007046820722
47	21.808635061464752	29.21907980373109	27.07723891584269	21.89504621896147
48	22.572484008890626	25.610359064961557	31.17397171181237	20.643185214335446
49	20.31414400208414	26.456858174706483	31.924978236680346	21.304019586529034
50	18.83882484495068	34.19295006040525	26.851487473592766	20.116737621051296
51	18.567299339440822	34.04471181145122	25.343328407346966	22.044660441760993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	699.0
1	1378.0
2	2057.0
3	6937.5
4	11818.0
5	8985.5
6	6153.0
7	6258.5
8	6364.0
9	6667.5
10	6971.0
11	6963.0
12	6955.0
13	7068.5
14	7182.0
15	6986.0
16	6790.0
17	6481.5
18	6173.0
19	5698.5
20	5224.0
21	5444.0
22	5664.0
23	5720.5
24	5777.0
25	6663.0
26	8481.5
27	9414.0
28	11716.5
29	14019.0
30	16290.5
31	18562.0
32	20744.0
33	22926.0
34	26210.5
35	29495.0
36	29526.5
37	29558.0
38	38870.5
39	48183.0
40	62459.0
41	76735.0
42	90409.0
43	104083.0
44	106670.0
45	109257.0
46	99954.0
47	90651.0
48	84279.0
49	77907.0
50	75591.0
51	73275.0
52	69398.5
53	65522.0
54	60116.5
55	54711.0
56	51299.0
57	47887.0
58	44620.0
59	41353.0
60	38316.5
61	35280.0
62	29140.5
63	23001.0
64	18506.5
65	14012.0
66	10566.5
67	7121.0
68	6157.0
69	5193.0
70	3968.0
71	2743.0
72	2221.5
73	1700.0
74	1333.5
75	769.0
76	571.0
77	441.0
78	311.0
79	245.5
80	180.0
81	125.5
82	71.0
83	54.0
84	37.0
85	32.0
86	27.0
87	16.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1090137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.140433941407146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01396874813858	15.315020832135623
2	5.595157704529026	2.141874928705861
3	2.095235580457104	1.2031115465827515
4	1.0901392844702655	0.8346295584534388
5	0.7833632074453478	0.7496955862118251
6	0.6175394884367825	0.7091984270780759
7	0.4950527830837096	0.6632867564486453
8	0.40720196691093424	0.6235217878775836
9	0.36749360641188394	0.6330588387674527
>10	5.434440048659988	25.511980749149497
>50	2.351668058368794	32.1359865733577
>100	0.7453689914723782	18.224861834017215
>500	0.001926018065820099	0.2912320903144123
>1k	9.630090329100495E-4	0.4068033059012075
>5k	4.8150451645502475E-4	0.5557371849987045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6030	0.5531414858866363	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2608	0.23923598593571269	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1806	0.16566725099689306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008806232611130527	0.0
2	0.0	0.0	0.0	0.03238125116384454	0.0
3	0.0	0.0	0.0	0.04329731033805843	0.0
4	0.0	0.0	0.0	0.06301960212340284	0.0
5	0.0	0.0	0.0	0.10365669636018225	0.0
6	0.0	0.0	0.0	0.15621889725786758	0.0
7	0.0	0.0	0.0	0.18850841683201286	0.0
8	0.0	0.0	0.0	0.2790474958651986	0.0
9	0.0	0.0	0.0	0.3172995687697968	0.0
10	0.0	0.0	0.0	0.3752739334597395	0.0
11	0.0	0.0	0.0	0.43508292994366765	0.0
12	0.0	0.0	0.0	0.4819577722799978	0.0
13	0.0	0.0	0.0	0.5038816222181248	0.0
14	0.0	0.0	0.0	0.5124126600601576	0.0
15	0.0	0.0	0.0	0.5254385457974549	0.0
16	0.0	0.0	0.0	0.5580032601406979	0.0
17	0.0	0.0	0.0	0.5933199221749192	0.0
18	0.0	0.0	0.0	0.6449648071756119	0.0
19	0.0	0.0	0.0	0.6684480941386266	0.0
20	0.0	0.0	0.0	0.6949585235617175	0.0
21	0.0	0.0	0.0	0.7299082592371418	0.0
22	0.0	0.0	0.0	0.7654083844507616	0.0
23	0.0	0.0	0.0	0.8087056947888202	0.0
24	0.0	0.0	0.0	0.8418207986702588	0.0
25	0.0	0.0	0.0	0.8692485439903425	0.0
26	0.0	0.0	0.0	0.8983274579250131	0.0
27	0.0	0.0	0.0	0.9281402245772779	0.0
28	0.0	0.0	0.0	0.9596041598441297	0.0
29	0.0	0.0	0.0	0.9903342423933873	0.0
30	0.0	0.0	0.0	1.0384015953958081	0.0
31	0.0	0.0	0.0	1.0821575636823628	0.0
32	0.0	0.0	0.0	1.1209600261251567	0.0
33	0.0	0.0	0.0	1.160588072875244	0.0
34	0.0	0.0	0.0	1.2064538677248824	0.0
35	0.0	0.0	0.0	1.265804206260314	0.0
36	0.0	0.0	0.0	1.3137798276730357	0.0
37	0.0	0.0	0.0	1.3624893018033513	0.0
38	9.173158969927633E-5	0.0	0.0	1.4083550966529894	0.0
39	9.173158969927633E-5	0.0	0.0	1.4596330552948849	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	25	3.890672E-5	45.000004	38
CGTCTAC	25	3.890672E-5	45.000004	44
CGTTCAC	45	3.8562575E-10	45.000004	21
CGTAGCG	25	3.890672E-5	45.000004	23
TTAACGA	25	3.890672E-5	45.000004	45
CACCGTA	25	3.890672E-5	45.000004	39
ACCGGAT	20	7.033126E-4	45.0	21
CGTATCC	20	7.033126E-4	45.0	16
CGACGTC	20	7.033126E-4	45.0	18
TCTACCG	20	7.033126E-4	45.0	45
CTATTAG	20	7.033126E-4	45.0	1
TCATACC	40	6.8139343E-9	45.0	37
CGCACGG	20	7.033126E-4	45.0	2
TCTAGCG	55	1.8189894E-12	44.999996	1
GTGACGG	105	0.0	42.85714	2
GCTACGA	190	0.0	42.63158	10
CGGGTAT	175	0.0	42.428574	6
TCGATAG	85	0.0	42.352943	1
CGGGCAC	75	0.0	42.0	6
TGCGATG	70	0.0	41.785713	1
>>END_MODULE