FastQCFastQC Report
Thu 26 May 2016
SRR1545466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545466_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1075164
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67690.6295783712996342No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30530.2839566800971759No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11310.10519325423842317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA253.890638E-545.037
CACGATC207.033085E-445.034
CGCTAAT207.033085E-445.035
GTCCGAT253.890638E-545.038
ACGTAAG502.1827873E-1145.01
TATCCGT253.890638E-545.015
TCTAGCG600.044.9999961
CGGTCTA1200.044.99999631
CGACGGT1250.043.19999728
GCGATAT1050.042.857149
CGTTTTT51300.041.3157921
TAGTACG1200.041.2499961
CGTAAGG1700.039.7058832
GCGTAAG850.039.7058831
CGTCTAA403.4579534E-739.37538
TCGATAG800.039.3751
TCGAACG403.4579534E-739.3751
AGGGCGA9500.039.315796
ACATACG2350.039.25531817
CGTGCGG750.039.02
Produced by FastQC (version 0.11.5)