##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545466_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1075164
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97704629247259	31.0	31.0	33.0	30.0	34.0
2	31.275466812504884	31.0	31.0	34.0	30.0	34.0
3	31.37416896399061	31.0	31.0	34.0	28.0	34.0
4	35.2160600615348	37.0	35.0	37.0	33.0	37.0
5	35.09764184812735	37.0	35.0	37.0	32.0	37.0
6	35.140012128382274	37.0	35.0	37.0	32.0	37.0
7	35.594649746457286	37.0	35.0	37.0	35.0	37.0
8	35.51834231800916	37.0	35.0	37.0	35.0	37.0
9	37.22631710139105	39.0	37.0	39.0	34.0	39.0
10	36.593333668165975	39.0	35.0	39.0	32.0	39.0
11	36.34901559203991	38.0	35.0	39.0	32.0	39.0
12	35.75915023196461	37.0	35.0	39.0	31.0	39.0
13	35.38455993690265	37.0	35.0	39.0	30.0	39.0
14	36.40839165001805	38.0	35.0	40.0	30.0	41.0
15	36.695253003262756	38.0	35.0	40.0	31.0	41.0
16	36.74851092484495	38.0	35.0	40.0	31.0	41.0
17	36.67720552399448	38.0	35.0	40.0	31.0	41.0
18	36.60847740437738	38.0	35.0	40.0	31.0	41.0
19	36.528727710377204	38.0	35.0	40.0	31.0	41.0
20	36.40167267505237	38.0	35.0	40.0	31.0	41.0
21	36.25006603643723	38.0	35.0	40.0	30.0	41.0
22	36.16260496073157	38.0	34.0	40.0	30.0	41.0
23	36.074727204407885	38.0	34.0	40.0	30.0	41.0
24	35.97351287803535	37.0	34.0	40.0	30.0	41.0
25	35.827816965597805	37.0	34.0	40.0	30.0	41.0
26	35.6934244450149	37.0	34.0	40.0	30.0	41.0
27	35.61129929945571	37.0	34.0	40.0	29.0	41.0
28	35.6628505046672	37.0	34.0	40.0	29.0	41.0
29	35.678590428995015	37.0	34.0	40.0	29.0	41.0
30	35.57945950571262	37.0	34.0	40.0	29.0	41.0
31	35.437483955936024	37.0	34.0	40.0	29.0	41.0
32	35.24322428950374	37.0	34.0	40.0	27.0	41.0
33	35.0198983596921	37.0	34.0	40.0	27.0	41.0
34	34.84170135904848	37.0	34.0	40.0	26.0	41.0
35	34.656491474788965	37.0	33.0	40.0	25.0	41.0
36	34.48618443325855	37.0	33.0	40.0	24.0	41.0
37	34.44357884006533	37.0	33.0	40.0	24.0	41.0
38	34.39260708133829	37.0	33.0	40.0	24.0	41.0
39	34.32902236309995	36.0	33.0	40.0	23.0	41.0
40	34.16974526676861	36.0	33.0	40.0	23.0	41.0
41	34.11101748198414	36.0	33.0	40.0	23.0	41.0
42	34.05412104571953	36.0	33.0	40.0	23.0	41.0
43	33.96402502315926	36.0	33.0	40.0	23.0	41.0
44	33.89495183990535	36.0	33.0	40.0	23.0	41.0
45	33.83000174857045	35.0	33.0	40.0	23.0	41.0
46	33.766998336997894	35.0	33.0	40.0	23.0	41.0
47	33.67650423563289	35.0	33.0	40.0	23.0	41.0
48	33.6224808494332	35.0	32.0	39.0	23.0	41.0
49	33.63135484447024	35.0	32.0	39.0	23.0	41.0
50	33.55384015833864	35.0	32.0	39.0	23.0	41.0
51	33.377545193105426	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	0.0
13	2.0
14	12.0
15	41.0
16	107.0
17	291.0
18	670.0
19	1300.0
20	2257.0
21	3607.0
22	5338.0
23	7781.0
24	10769.0
25	15294.0
26	19912.0
27	23654.0
28	25628.0
29	28473.0
30	33772.0
31	41294.0
32	50371.0
33	63593.0
34	99326.0
35	126947.0
36	85815.0
37	103168.0
38	140135.0
39	185546.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.16281423113125	23.565149130737264	27.82301118713052	16.449025451000963
2	31.578903311494805	25.75858194656815	28.810395437347232	13.852119304589811
3	28.593591303280242	25.15448805949604	32.81099441573564	13.44092622148807
4	25.988686377148046	27.810361954083284	31.25327856959497	14.94767309917371
5	22.57748585332098	31.563742833651425	31.466269331934477	14.392501981093117
6	21.458028728640468	39.80416010952747	28.71106175429981	10.026749407532247
7	85.20886115978585	4.746531691909327	8.343750348784	1.7008567995208173
8	86.4374179194988	3.7486374171754258	7.966970620296067	1.8469740430297148
9	81.42990278692366	5.468096030001004	9.735817047445785	3.3661841356295414
10	44.775122678958745	23.672202566306165	17.648284354758903	13.90439039997619
11	39.40682537733778	23.52720143159555	22.49582389291308	14.570149298153584
12	35.17714506810124	22.633477311368313	26.595663545282395	15.593714075248055
13	22.427462229018087	32.91060712598264	27.183387836646318	17.47854280835296
14	17.993999055027885	35.01456521981763	29.314504577906252	17.676931147248233
15	17.864251407227176	23.505065273762888	42.0043825872146	16.626300731795336
16	19.174749154547584	19.65839630047137	40.99272297063518	20.174131574345864
17	19.658024264205277	20.56383956308061	29.96212670811151	29.81600946460261
18	22.493684684383034	23.702244494793355	33.106484220081775	20.69758660074184
19	28.88508171776585	23.25849823840828	27.12404805220413	20.732371991621743
20	30.592821188209427	22.597947847956217	27.271839458910456	19.5373915049239
21	24.28373717870018	26.16307837688018	29.221681529515497	20.331502914904146
22	23.723450561960778	24.43896931072841	27.396006562719734	24.441573564591078
23	22.157549917966005	28.286382356552114	27.288860118084312	22.26720760739757
24	21.159004579766435	23.578170400050595	36.47434251890874	18.788482501274224
25	20.521613446878803	24.631033033100067	33.75726865854883	21.090084861472295
26	19.764054600042414	30.54947896320933	28.60038096513648	21.086085471611774
27	19.278454263721628	30.480094199582574	30.6220260350979	19.619425501597895
28	18.97756993351712	27.47069284313835	35.116317138594674	18.43542008474986
29	18.726910499235466	25.361898277844126	34.76055745914112	21.150633763779293
30	20.153111525311488	28.633771220018527	31.84593234148465	19.367184913185337
31	26.479030175861546	26.661699982514296	27.454602274629732	19.40466756699443
32	28.33735132500716	25.732353389808438	28.069671231551652	17.860624053632748
33	26.78103061486434	27.284581701024212	26.126339795603275	19.80804788850817
34	20.93643388357497	27.15539210762265	29.006272531446363	22.901901477356013
35	22.278275686313904	25.696079853864155	29.980635512349746	22.0450089474722
36	27.434791343460162	26.969932028974185	26.16307837688018	19.432198250685477
37	21.792117295593975	31.41371920934853	28.475748815994585	18.318414679062915
38	22.113835656699816	30.73670621412175	25.870843889862382	21.27861423931605
39	21.789699059864358	30.177163669914542	27.433210189329255	20.599927080891845
40	24.053446729987236	25.763790454293485	27.608253252527053	22.57450956319222
41	19.083321242154685	25.980501579293954	28.53741382709987	26.3987633514515
42	22.14573776651748	27.053640188845606	25.6771060042933	25.123516040343613
43	22.25427934715076	26.638912761215966	27.84254309110052	23.264264800532754
44	21.012608309057967	29.034082242336982	28.366556171895635	21.586753276709413
45	19.826277665546836	33.0776514094594	24.839373342113387	22.25669758288038
46	21.295169853157287	30.987644675602976	26.71266895096934	21.004516520270396
47	22.474524816679132	27.936947293622183	27.70488967264529	21.8836382170534
48	22.838934339319398	25.962550829454855	29.9570112094527	21.24150362177305
49	21.410501095646804	26.649236767600105	30.51153126406762	21.42873087268547
50	20.92341261426164	31.168175273725684	27.36103515370678	20.547376958305897
51	19.62342489145842	30.82459978198675	26.625705473769585	22.92626985278525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	591.0
1	1198.0
2	1805.0
3	7053.0
4	12301.0
5	9074.5
6	5848.0
7	5978.0
8	6108.0
9	6294.0
10	6480.0
11	6502.0
12	6524.0
13	6340.5
14	6157.0
15	5818.5
16	5480.0
17	5243.0
18	5006.0
19	4745.5
20	4485.0
21	4851.0
22	5217.0
23	5217.0
24	5217.0
25	5522.5
26	7516.5
27	9205.0
28	10562.0
29	11919.0
30	13550.5
31	15182.0
32	18924.5
33	22667.0
34	26140.0
35	29613.0
36	30339.0
37	31065.0
38	36381.0
39	41697.0
40	54566.5
41	67436.0
42	76386.5
43	85337.0
44	86487.0
45	87637.0
46	86554.5
47	85472.0
48	82429.5
49	79387.0
50	78686.5
51	77986.0
52	73280.5
53	68575.0
54	65849.5
55	63124.0
56	58832.0
57	54540.0
58	52629.5
59	50719.0
60	45046.5
61	39374.0
62	35736.5
63	32099.0
64	25767.5
65	19436.0
66	15020.0
67	10604.0
68	8457.0
69	6310.0
70	4937.5
71	3565.0
72	2929.5
73	2294.0
74	1883.5
75	1051.0
76	629.0
77	504.5
78	380.0
79	303.5
80	227.0
81	162.5
82	98.0
83	64.5
84	31.0
85	28.0
86	25.0
87	14.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1075164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.99043894895823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84785747083059	15.362224031288827
2	5.607689952173948	2.242003672672396
3	2.290005201040379	1.3733462749258354
4	1.4254983863431705	1.139853538561265
5	1.0371617403975668	1.036665922580641
6	0.8150437273436829	0.9775849123317147
7	0.7049068689419548	0.9863978409799811
8	0.6140263857186113	0.9819725581405903
9	0.5361181499296706	0.9645513431037762
>10	7.663629435007284	37.31881096914187
>50	2.2248295051620595	30.416913182435145
>100	0.23183097364151573	6.1762762530685755
>500	0.0	0.0
>1k	9.348023130706278E-4	0.39093431125894706
>5k	4.674011565353139E-4	0.6324651895104716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6769	0.6295783712996342	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3053	0.2839566800971759	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1131	0.10519325423842317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005673553057952089	0.0
2	0.0	0.0	0.0	0.02920484688847469	0.0
3	0.0	0.0	0.0	0.042505143401378766	0.0
4	0.0	0.0	0.0	0.06510634656666332	0.0
5	0.0	0.0	0.0	0.10770449903456589	0.0
6	0.0	0.0	0.0	0.16378896614842015	0.0
7	0.0	0.0	0.0	0.19792329356265648	0.0
8	0.0	0.0	0.0	0.2885141243568423	0.0
9	0.0	0.0	0.0	0.33139130402431627	0.0
10	0.0	0.0	0.0	0.404403421245503	0.0
11	0.0	0.0	0.0	0.46458028728640466	0.0
12	0.0	0.0	0.0	0.516851382672783	0.0
13	0.0	0.0	0.0	0.5392665677050199	0.0
14	0.0	0.0	0.0	0.5494975650226384	0.0
15	0.0	0.0	0.0	0.5622398071363996	0.0
16	0.0	0.0	0.0	0.5881893366965412	0.0
17	0.0	0.0	0.0	0.6247418998403964	0.0
18	0.0	0.0	0.0	0.6776640586924413	0.0
19	0.0	0.0	0.0	0.7001722527912021	0.0
20	0.0	0.0	0.0	0.7278889546152959	0.0
21	0.0	0.0	0.0	0.7607211550981989	0.0
22	0.0	0.0	0.0	0.793088310248483	0.0
23	0.0	0.0	0.0	0.8301989277914811	0.0
24	0.0	0.0	0.0	0.8616359922765271	0.0
25	9.300906652380474E-5	0.0	0.0	0.8877715399697163	0.0
26	9.300906652380474E-5	0.0	0.0	0.9157672689933815	0.0
27	9.300906652380474E-5	0.0	0.0	0.9459022065470942	0.0
28	9.300906652380474E-5	0.0	0.0	0.976967234766045	0.0
29	9.300906652380474E-5	0.0	0.0	1.011287580313329	0.0
30	9.300906652380474E-5	0.0	0.0	1.0605823855709455	0.0
31	9.300906652380474E-5	0.0	0.0	1.102715492706229	0.0
32	9.300906652380474E-5	0.0	0.0	1.1454996633071792	0.0
33	9.300906652380474E-5	0.0	0.0	1.1853075437793676	0.0
34	9.300906652380474E-5	0.0	0.0	1.2321841133073652	0.0
35	9.300906652380474E-5	0.0	0.0	1.2873384897559814	0.0
36	9.300906652380474E-5	0.0	0.0	1.3322618688869792	0.0
37	9.300906652380474E-5	0.0	0.0	1.3815566741445957	0.0
38	9.300906652380474E-5	0.0	0.0	1.436618041526688	0.0
39	9.300906652380474E-5	0.0	0.0	1.512327421677065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	25	3.890638E-5	45.0	37
CACGATC	20	7.033085E-4	45.0	34
CGCTAAT	20	7.033085E-4	45.0	35
GTCCGAT	25	3.890638E-5	45.0	38
ACGTAAG	50	2.1827873E-11	45.0	1
TATCCGT	25	3.890638E-5	45.0	15
TCTAGCG	60	0.0	44.999996	1
CGGTCTA	120	0.0	44.999996	31
CGACGGT	125	0.0	43.199997	28
GCGATAT	105	0.0	42.85714	9
CGTTTTT	5130	0.0	41.315792	1
TAGTACG	120	0.0	41.249996	1
CGTAAGG	170	0.0	39.705883	2
GCGTAAG	85	0.0	39.705883	1
CGTCTAA	40	3.4579534E-7	39.375	38
TCGATAG	80	0.0	39.375	1
TCGAACG	40	3.4579534E-7	39.375	1
AGGGCGA	950	0.0	39.31579	6
ACATACG	235	0.0	39.255318	17
CGTGCGG	75	0.0	39.0	2
>>END_MODULE