FastQCFastQC Report
Thu 26 May 2016
SRR1545465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545465_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences925367
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.6640608536937237No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42310.45722399869457203No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11810.1276250395788914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC502.1827873E-1145.00000425
CTATGCG502.1827873E-1145.0000041
TCGTTGA1150.045.00000424
TTGGCAT253.8902137E-545.00000428
CGACGGT502.1827873E-1145.00000428
CGTTGAT1150.045.00000425
ATTACGG253.8902137E-545.0000042
TTCACGA253.8902137E-545.00000427
CACGACG502.1827873E-1145.00000426
TCTAGTC253.8902137E-545.00000422
TATGCGC253.8902137E-545.0000041
ATGTACG253.8902137E-545.0000041
AGACGAT253.8902137E-545.00000442
CTAACGG650.045.0000042
CGTAGCC253.8902137E-545.00000440
GTATCGC253.8902137E-545.00000432
CGTAACA253.8902137E-545.00000435
TGCTACG253.8902137E-545.0000041
ATTGCGA253.8902137E-545.00000411
TTACCCG253.8902137E-545.00000444
Produced by FastQC (version 0.11.5)