FastQCFastQC Report
Thu 26 May 2016
SRR1545464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545464_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1258260
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.7024780252094162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33650.26743280403096337No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17800.14146519797180235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC551.8189894E-1245.0000046
TATATCG207.033542E-445.042
CGCGATC207.033542E-445.019
CGTTAGA207.033542E-445.027
CTCGACT207.033542E-445.014
CGCACGT207.033542E-445.029
TATCGGC207.033542E-445.011
TCGAATC207.033542E-445.020
GACGCAT302.165667E-644.99999610
CCCGAGT600.044.99999645
CGTTTTT65750.041.714831
ACGTTAG650.041.5384641
CGTTAGG2550.041.470592
TACGGGA3700.041.3513534
AGTACGG1050.040.7142872
AAATGCG501.0822987E-940.51
CGGGTAT1450.040.3448266
CGTATGA950.040.26315715
GCGATAT950.040.2631579
GGTTAAC3200.040.0781258