##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545464_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1258260
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.022681321825377	31.0	31.0	33.0	30.0	34.0
2	31.315924371751468	31.0	31.0	34.0	30.0	34.0
3	31.404043679366744	31.0	31.0	34.0	30.0	34.0
4	35.246463370050705	37.0	35.0	37.0	33.0	37.0
5	35.13229936579086	37.0	35.0	37.0	32.0	37.0
6	35.191629710870565	37.0	35.0	37.0	32.0	37.0
7	35.630092349752836	37.0	35.0	37.0	35.0	37.0
8	35.55854195476293	37.0	35.0	37.0	35.0	37.0
9	37.2644493189007	39.0	37.0	39.0	34.0	39.0
10	36.68475513804778	39.0	37.0	39.0	32.0	39.0
11	36.43246944192774	38.0	35.0	39.0	32.0	39.0
12	35.73430451575986	37.0	35.0	39.0	31.0	39.0
13	35.32960993753278	37.0	35.0	39.0	30.0	39.0
14	36.35045459602944	38.0	35.0	40.0	30.0	41.0
15	36.66644493189007	38.0	35.0	40.0	31.0	41.0
16	36.746891739386136	38.0	35.0	40.0	31.0	41.0
17	36.65177626245768	38.0	35.0	40.0	31.0	41.0
18	36.60700888528603	38.0	35.0	40.0	31.0	41.0
19	36.5253095544641	38.0	35.0	40.0	31.0	41.0
20	36.37669321126	38.0	35.0	40.0	31.0	41.0
21	36.225259485321	38.0	35.0	40.0	31.0	41.0
22	36.11693211259994	37.0	34.0	40.0	30.0	41.0
23	36.038654967971645	37.0	34.0	40.0	30.0	41.0
24	35.918581215329105	37.0	34.0	40.0	30.0	41.0
25	35.788015195587555	37.0	34.0	40.0	30.0	41.0
26	35.64187608284456	37.0	34.0	40.0	30.0	41.0
27	35.57602482793699	37.0	34.0	40.0	29.0	41.0
28	35.61469012763658	37.0	34.0	40.0	29.0	41.0
29	35.65064851461542	37.0	34.0	40.0	30.0	41.0
30	35.54336305691987	37.0	34.0	40.0	29.0	41.0
31	35.40431945702796	37.0	34.0	40.0	29.0	41.0
32	35.19305866831974	37.0	34.0	40.0	28.0	41.0
33	34.951253318074166	37.0	34.0	40.0	27.0	41.0
34	34.77799024049084	36.0	34.0	40.0	26.0	41.0
35	34.57359766661898	36.0	33.0	40.0	25.0	41.0
36	34.39067124441689	36.0	33.0	40.0	24.0	41.0
37	34.334545324495735	36.0	33.0	40.0	23.0	41.0
38	34.3277088995915	36.0	33.0	40.0	24.0	41.0
39	34.27114427860697	36.0	33.0	40.0	23.0	41.0
40	34.1124195317343	36.0	33.0	40.0	23.0	41.0
41	34.04578306550316	36.0	33.0	40.0	23.0	41.0
42	33.98968178277939	36.0	33.0	40.0	23.0	41.0
43	33.89870853400728	35.0	33.0	40.0	23.0	41.0
44	33.82233004307536	35.0	33.0	40.0	23.0	41.0
45	33.7395593915407	35.0	33.0	40.0	23.0	41.0
46	33.67040754692989	35.0	33.0	40.0	23.0	41.0
47	33.61721265875097	35.0	33.0	40.0	23.0	41.0
48	33.583694943811295	35.0	33.0	39.0	23.0	41.0
49	33.58566989334478	35.0	33.0	39.0	23.0	41.0
50	33.51099295853003	35.0	32.0	39.0	23.0	41.0
51	33.33633748191948	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	8.0
14	15.0
15	53.0
16	139.0
17	368.0
18	768.0
19	1541.0
20	2766.0
21	4208.0
22	6270.0
23	8913.0
24	12846.0
25	17788.0
26	23905.0
27	27693.0
28	30035.0
29	33247.0
30	38756.0
31	47536.0
32	58517.0
33	75258.0
34	120264.0
35	160376.0
36	95859.0
37	115117.0
38	157926.0
39	218001.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42925945353107	22.77867849252142	27.351262855053804	16.44079919889371
2	32.54692988730469	25.002066345588354	29.05130895045539	13.399694816651566
3	30.101489358320222	24.704353631205	31.867181663567145	13.326975346907636
4	26.23138302099725	27.238567545658288	31.70092031853512	14.829129114809339
5	22.418816460826857	31.835073832117367	31.383100472080493	14.363009234975282
6	21.02029787166404	39.83373865496797	29.34115365663694	9.80480981673104
7	84.90669654920286	4.491996884586651	8.8166992513471	1.7846073148633828
8	85.86174558517318	3.971039371830941	8.44873078695977	1.7184842560361135
9	80.64438192424458	5.7407848934242525	10.457854497480648	3.156978684850508
10	45.54146201897859	25.728466294724463	17.1428003751212	11.587271311175751
11	39.60445376949121	22.13000492743948	23.727290067235707	14.53825123583361
12	36.7251601417831	21.70552985869375	27.600098548789596	13.969211450733551
13	21.397882790520242	36.613974854163686	26.10303117002845	15.88511118528762
14	16.043901896269453	39.09144373976762	28.715686742008806	16.148967621954128
15	15.414779139446539	23.73571439924976	45.582629981084985	15.266876480218713
16	17.389887622589924	18.821308791505732	43.87892804348863	19.909875542415715
17	16.510578099915758	20.069461001700763	31.17606853909367	32.24389235928981
18	20.852844404177198	22.907586667302464	35.77011110581279	20.469457822707547
19	28.037448540047365	23.696533307901387	27.497178643523597	20.768839508527652
20	30.4344888973662	21.648705355014066	28.09419356889673	19.822612178723
21	22.728609349419038	27.016832769062038	29.110120324893106	21.144437556625817
22	23.45453244957322	24.436205553701143	26.706483556657606	25.40277844006803
23	20.28006930205204	28.39977429148189	27.376694800756603	23.94346160570947
24	19.197224738925183	23.46279783192663	39.56455740466994	17.775420024478247
25	18.422663042614403	25.30756759334319	36.052962026926075	20.216807337116336
26	17.944065614419912	31.89936896984725	28.973185192249613	21.183380223483223
27	18.187814919015146	32.72558930586683	29.20437747365409	19.88221830146393
28	16.265239298714096	28.422663042614403	36.608173191550236	18.703924467121265
29	16.81528459936738	24.154228855721392	36.71912005467868	22.311366490232544
30	18.173906823708933	30.09521084672484	32.57124918538299	19.15963314418324
31	26.43356698933448	28.035461669289337	26.851684071654507	18.679287269721677
32	27.813408993371798	25.692941045570866	28.646464164799006	17.847185796258323
33	26.459714208510164	27.978557690779333	26.22566083321412	19.336067267496386
34	19.771033013844516	27.56552699759986	29.130306931794703	23.533133056760924
35	21.14411965730453	25.691748923116048	31.411949835487103	21.752181584092316
36	27.67361276683674	27.52205426541414	26.25506652043298	18.549266447316135
37	20.071845246610398	33.11048590911258	28.78268402396961	18.034984820307407
38	20.17786467025893	31.77880565224993	27.19485638898161	20.848473288509528
39	20.430833055171426	32.25231669130386	27.980385611876716	19.33646464164799
40	23.35550681099296	26.813695102760953	27.916726272789404	21.91407181345668
41	17.51013304086596	26.500723220955923	29.295376154371912	26.69376758380621
42	20.87994532131674	26.831815364074195	26.27112043615787	26.017118878451196
43	21.36386756314275	27.489072210830827	27.708661167008408	23.438399059018007
44	19.974488579466882	29.58196239251029	28.831163670465564	21.61238535755726
45	17.89367857199625	36.24298634622415	24.759668113108578	21.10366696867102
46	20.34468233910321	33.296218587573314	26.815681973518984	19.54341709980449
47	20.886621207063722	29.36555242954556	27.7299604215345	22.01786594185621
48	21.765294931095323	25.65821054472049	31.189499785417958	21.386994738766234
49	20.380604962408405	26.733107624815222	32.76413459857263	20.12215281420374
50	19.388838554829686	33.16246244814267	27.887320585570553	19.561378411457092
51	18.727051642744744	32.08653219525377	26.893249407912514	22.29316675408898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	937.0
1	1641.0
2	2345.0
3	8889.5
4	15434.0
5	11324.5
6	7215.0
7	7490.0
8	7765.0
9	8019.5
10	8274.0
11	8193.5
12	8113.0
13	8022.5
14	7932.0
15	7649.5
16	7367.0
17	6696.5
18	6026.0
19	6142.0
20	6258.0
21	6128.0
22	5998.0
23	6601.0
24	7204.0
25	7433.5
26	8889.5
27	10116.0
28	14800.0
29	19484.0
30	21690.0
31	23896.0
32	28105.0
33	32314.0
34	37450.0
35	42586.0
36	43922.0
37	45258.0
38	50175.0
39	55092.0
40	66391.5
41	77691.0
42	92847.0
43	108003.0
44	108807.0
45	109611.0
46	107735.0
47	105859.0
48	97906.0
49	89953.0
50	85394.0
51	80835.0
52	77757.5
53	74680.0
54	68360.0
55	62040.0
56	59283.5
57	56527.0
58	52647.0
59	48767.0
60	45119.5
61	41472.0
62	36037.0
63	30602.0
64	25037.5
65	19473.0
66	14851.0
67	10229.0
68	8273.5
69	6318.0
70	5048.5
71	3779.0
72	3094.5
73	2410.0
74	1931.0
75	1004.0
76	556.0
77	447.0
78	338.0
79	282.5
80	227.0
81	158.5
82	90.0
83	53.0
84	16.0
85	27.5
86	39.0
87	23.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1258260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.18712469114372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91564817328224	14.53435858101056
2	5.314538105668292	1.9331233440724793
3	1.920588616310335	1.0478995393568171
4	1.1340932868134332	0.8250358407465972
5	0.7679782125107533	0.6983657755507369
6	0.635940225455983	0.6939554505889207
7	0.5129218468824737	0.6530001510244292
8	0.43696592576133403	0.6357723022081945
9	0.4013197387843853	0.6568966917259957
>10	5.670304931522304	25.092255238603883
>50	2.4410712459203587	31.90702113024529
>100	0.8451211445616634	19.875894321228834
>500	0.0013157049474234511	0.15641517721201245
>1k	0.0017542732632312681	0.5849815959576233
>5k	4.3856831580781704E-4	0.7050248604676074
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8839	0.7024780252094162	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3365	0.26743280403096337	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1780	0.14146519797180235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.947483032123727E-5	0.0	0.0	0.008742231335336099	0.0
2	7.947483032123727E-5	0.0	0.0	0.03337942873491965	0.0
3	7.947483032123727E-5	0.0	0.0	0.04617487641663885	0.0
4	7.947483032123727E-5	0.0	0.0	0.06381828874795352	0.0
5	7.947483032123727E-5	0.0	0.0	0.09807194061640678	0.0
6	7.947483032123727E-5	0.0	0.0	0.14615421296075534	0.0
7	7.947483032123727E-5	0.0	0.0	0.17540095051897064	0.0
8	7.947483032123727E-5	0.0	0.0	0.25185573728800087	0.0
9	7.947483032123727E-5	0.0	0.0	0.28094352518557375	0.0
10	7.947483032123727E-5	0.0	0.0	0.3311716179485957	0.0
11	7.947483032123727E-5	0.0	0.0	0.38139971071161766	0.0
12	7.947483032123727E-5	0.0	0.0	0.42407769459412203	0.0
13	7.947483032123727E-5	0.0	0.0	0.43925738718547835	0.0
14	7.947483032123727E-5	0.0	0.0	0.44601274776278355	0.0
15	7.947483032123727E-5	0.0	0.0	0.456900799516793	0.0
16	7.947483032123727E-5	0.0	0.0	0.4824916948802314	0.0
17	7.947483032123727E-5	0.0	0.0	0.5126126555719803	0.0
18	7.947483032123727E-5	0.0	0.0	0.5540190421693449	0.0
19	7.947483032123727E-5	0.0	0.0	0.5715829796703384	0.0
20	7.947483032123727E-5	0.0	0.0	0.5943127811422123	0.0
21	7.947483032123727E-5	0.0	0.0	0.621016324130148	0.0
22	7.947483032123727E-5	0.0	0.0	0.6508988603309332	0.0
23	7.947483032123727E-5	0.0	0.0	0.6867420088058112	0.0
24	7.947483032123727E-5	0.0	0.0	0.7151145232304928	0.0
25	7.947483032123727E-5	0.0	0.0	0.7387980226662216	0.0
26	7.947483032123727E-5	0.0	0.0	0.7625609969322715	0.0
27	7.947483032123727E-5	0.0	0.0	0.7858471222163941	0.0
28	7.947483032123727E-5	0.0	0.0	0.8136633128288271	0.0
29	7.947483032123727E-5	0.0	0.0	0.8451353456360371	0.0
30	7.947483032123727E-5	0.0	0.0	0.8824090410566974	0.0
31	7.947483032123727E-5	0.0	0.0	0.9161858439432232	0.0
32	7.947483032123727E-5	0.0	0.0	0.948293675393003	0.0
33	7.947483032123727E-5	0.0	0.0	0.9850110470014146	0.0
34	7.947483032123727E-5	0.0	0.0	1.0228410662343237	0.0
35	7.947483032123727E-5	0.0	0.0	1.0694927916328898	0.0
36	7.947483032123727E-5	0.0	0.0	1.1091507319631873	0.0
37	7.947483032123727E-5	0.0	0.0	1.1527029389792254	0.0
38	7.947483032123727E-5	0.0	0.0	1.1960961963346208	0.0
39	7.947483032123727E-5	0.0	0.0	1.237661532592628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	55	1.8189894E-12	45.000004	6
TATATCG	20	7.033542E-4	45.0	42
CGCGATC	20	7.033542E-4	45.0	19
CGTTAGA	20	7.033542E-4	45.0	27
CTCGACT	20	7.033542E-4	45.0	14
CGCACGT	20	7.033542E-4	45.0	29
TATCGGC	20	7.033542E-4	45.0	11
TCGAATC	20	7.033542E-4	45.0	20
GACGCAT	30	2.165667E-6	44.999996	10
CCCGAGT	60	0.0	44.999996	45
CGTTTTT	6575	0.0	41.71483	1
ACGTTAG	65	0.0	41.538464	1
CGTTAGG	255	0.0	41.47059	2
TACGGGA	370	0.0	41.351353	4
AGTACGG	105	0.0	40.714287	2
AAATGCG	50	1.0822987E-9	40.5	1
CGGGTAT	145	0.0	40.344826	6
CGTATGA	95	0.0	40.263157	15
GCGATAT	95	0.0	40.263157	9
GGTTAAC	320	0.0	40.078125	8
>>END_MODULE