FastQCFastQC Report
Thu 26 May 2016
SRR1545463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545463_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1474696
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122900.8333921025079066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18480.12531396301339395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT207.033937E-445.044
TAGCCGA207.033937E-445.039
GGGCGTA207.033937E-445.08
TAATGCG351.2122473E-745.01
CGTTTTT97750.042.053711
CGATCTC556.184564E-1140.90909210
TACGGGA3700.040.7432444
CGACGGT750.039.028
TAACGGG4350.038.2758643
ACGGGAT5050.037.871295
CGTTAGG2450.037.653062
AGGGCGA15550.037.620586
CGAACGC301.1400785E-437.50000441
CCACTCG301.1400785E-437.50000429
CGTAAGG1800.037.52
GGGCGAT33900.037.3008847
GGCACCG7250.037.2413838
CGGTCTA850.037.05882331
GCGATGT4450.036.910119
GACCGAT8650.036.67639