##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545463_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1474696
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.874948463954606	31.0	31.0	33.0	28.0	34.0
2	31.157844735457342	31.0	31.0	34.0	28.0	34.0
3	31.225209805953227	31.0	31.0	34.0	28.0	34.0
4	35.08137134704373	35.0	35.0	37.0	32.0	37.0
5	34.95683856198159	35.0	35.0	37.0	32.0	37.0
6	35.006391825840716	37.0	35.0	37.0	32.0	37.0
7	35.5661180338185	37.0	35.0	37.0	35.0	37.0
8	35.49334506908542	37.0	35.0	37.0	35.0	37.0
9	37.17782987137688	39.0	37.0	39.0	34.0	39.0
10	36.48155619870129	38.0	35.0	39.0	32.0	39.0
11	36.20597533322122	38.0	35.0	39.0	31.0	39.0
12	35.805655538497426	37.0	35.0	39.0	30.0	39.0
13	35.52482274312808	37.0	35.0	39.0	30.0	39.0
14	36.56296416346149	38.0	35.0	40.0	30.0	41.0
15	36.784570514872215	38.0	35.0	40.0	31.0	41.0
16	36.776025702924535	38.0	35.0	40.0	31.0	41.0
17	36.66114304236263	38.0	35.0	40.0	31.0	41.0
18	36.57114890119726	38.0	35.0	40.0	31.0	41.0
19	36.49224992812078	38.0	35.0	40.0	30.0	41.0
20	36.35894109701254	38.0	35.0	40.0	30.0	41.0
21	36.21390713747105	38.0	34.0	40.0	30.0	41.0
22	36.11323147279168	38.0	34.0	40.0	30.0	41.0
23	36.007252342177644	38.0	34.0	40.0	30.0	41.0
24	35.90824346170329	38.0	34.0	40.0	30.0	41.0
25	35.77942572570889	37.0	34.0	40.0	29.0	41.0
26	35.63598328062191	37.0	34.0	40.0	29.0	41.0
27	35.541449898826606	37.0	34.0	40.0	29.0	41.0
28	35.53854218089695	37.0	34.0	40.0	29.0	41.0
29	35.540554120984936	37.0	34.0	40.0	29.0	41.0
30	35.39596296457032	37.0	34.0	40.0	28.0	41.0
31	35.2498284392173	37.0	34.0	40.0	27.0	41.0
32	35.030320825444704	37.0	34.0	40.0	26.0	41.0
33	34.76055268340051	37.0	33.0	40.0	25.0	41.0
34	34.547359591400536	37.0	33.0	40.0	24.0	41.0
35	34.316659840401	37.0	33.0	40.0	23.0	41.0
36	34.15259416177978	37.0	33.0	40.0	23.0	41.0
37	34.12338882047554	37.0	33.0	40.0	23.0	41.0
38	34.05643875076626	37.0	33.0	40.0	23.0	41.0
39	33.96461575809523	37.0	33.0	40.0	22.0	41.0
40	33.830590169092474	37.0	33.0	40.0	21.0	41.0
41	33.74655590033471	36.0	33.0	40.0	21.0	41.0
42	33.696896851961355	36.0	33.0	40.0	21.0	41.0
43	33.59685114762636	36.0	32.0	40.0	21.0	41.0
44	33.51097717766916	36.0	32.0	40.0	20.0	41.0
45	33.41967157976966	36.0	32.0	40.0	20.0	41.0
46	33.36598865122032	36.0	32.0	40.0	20.0	41.0
47	33.28197811616767	35.0	32.0	40.0	20.0	41.0
48	33.19917528765251	35.0	32.0	40.0	20.0	41.0
49	33.2144001204316	36.0	32.0	40.0	20.0	41.0
50	33.13606600953688	35.0	32.0	39.0	20.0	41.0
51	32.95496359927741	35.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	4.0
13	10.0
14	16.0
15	66.0
16	222.0
17	591.0
18	1271.0
19	2529.0
20	4149.0
21	6374.0
22	9070.0
23	12543.0
24	17849.0
25	24625.0
26	31397.0
27	35951.0
28	38029.0
29	42141.0
30	48577.0
31	57412.0
32	68842.0
33	85197.0
34	120815.0
35	151858.0
36	121353.0
37	147539.0
38	196018.0
39	250185.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.52501396898073	22.49161861156469	26.011327080293157	16.972040339161428
2	34.951406934039284	24.47609541220699	27.64691841572772	12.925579238026005
3	28.884665042829166	24.990099654437252	32.88284500669968	13.242390296033896
4	25.826814475661426	27.51536587879807	31.75590087719774	14.901918768342764
5	22.662636909573227	30.827641764811187	31.74037225299316	14.769349072622425
6	21.068342221040812	39.826242154315196	29.113661391907215	9.991754232736781
7	84.0336584624899	4.130071553730396	10.075907170020127	1.7603628137595817
8	84.53152378524116	3.58392509371423	10.02593076810407	1.8586203529405383
9	79.59708305983064	5.582777738598328	11.458429398330232	3.361709803240803
10	38.83803848386379	30.809671959508943	18.274207023006774	12.078082533620488
11	32.45434991347369	25.57652560256487	27.344754444305806	14.624370039655632
12	29.005028833061186	24.639722356336495	30.939054557685107	15.416194252917212
13	22.019114447994706	30.863784807173815	30.95492223481992	16.162178510011554
14	18.85615747245534	33.201283518772684	31.93030970450859	16.01224930426339
15	18.414642746708473	24.86241232091224	40.186180745048475	16.536764187330814
16	20.778655397451406	22.85596489039097	38.531941498451204	17.83343821370642
17	20.9299408149205	22.417230398671997	31.904134818294754	24.74869396811275
18	23.215360996435873	23.52328886767171	34.60021590890597	18.66113422698644
19	27.238291824213263	24.33179448510066	29.606779973635245	18.823133717050837
20	28.557750207500394	24.136025323185255	29.675065233783776	17.63115923553058
21	24.552992616783392	26.36245029484043	31.225486473144297	17.859070615231882
22	23.968533175651118	24.52173193661609	29.85801819493645	21.651716692796345
23	20.855688223199902	27.94413221436825	30.49957414951963	20.70060541291222
24	19.799877398460428	24.932392845711927	37.126431481471435	18.141298274356206
25	19.90423789038554	26.846007583935943	34.59309579737112	18.65665872830739
26	20.048606628077923	29.633632965709545	30.40870796421771	19.909052441994824
27	20.019922750180378	29.52506821744956	31.262307621367384	19.192701411002673
28	18.3275061436391	27.973155145196028	35.312430494149304	18.386908217015574
29	19.15418499812843	26.26493867210598	34.76655527647733	19.81432105328827
30	20.213793215686486	28.23890483191112	33.00490406158286	18.542397890819533
31	24.96670500225131	26.637964705946178	30.08206437123312	18.313265920569393
32	25.757783299066382	25.850073506675276	30.180593152758263	18.21155004150008
33	24.479485941509303	27.08605705853952	28.61267678219782	19.821780217753354
34	20.691247552037844	27.376693230333576	30.5385652364962	21.393493981132384
35	20.886881092781156	27.255786955413186	31.469333340566465	20.387998611239198
36	25.140503534287745	28.324820844431663	28.025843970553932	18.50883165072666
37	20.92960176199027	30.987810369052333	28.678859914178922	19.40372795477848
38	21.331786347830334	30.658250920867758	28.11955820046979	19.890404530832118
39	21.38230523443476	30.50167627768706	27.763349191969056	20.352669295909124
40	23.784291813363566	27.2214747988738	28.525743610886583	20.468489776876048
41	19.53439895408952	26.892864698893877	29.13285178775829	24.439884559258314
42	21.74610902857267	27.22432284348774	28.026115212898116	23.00345291504147
43	21.552170752480514	27.032554506149065	28.69771125709977	22.717563484270656
44	20.560847795070984	29.031135908688977	29.616273455681714	20.791742840558324
45	19.84056375008815	32.41081551723203	27.17556703212052	20.573053700559303
46	21.065901039943146	30.80940071716476	28.022928115353945	20.101770127538153
47	20.940926129859985	28.976616197507827	29.054869613805153	21.02758805882704
48	21.561257371010704	27.659531184732312	30.811977519434514	19.967233924822473
49	21.62913576764296	27.633085056174288	30.875651659731908	19.862127516450848
50	20.34507451027195	31.042330080233487	28.802953286643486	19.80964212285108
51	20.195280925695872	30.725654643397686	27.871439266126714	21.207625164779724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	855.0
1	1547.0
2	2239.0
3	12117.5
4	21996.0
5	16055.5
6	10115.0
7	10248.5
8	10382.0
9	10684.5
10	10987.0
11	10956.0
12	10925.0
13	10729.0
14	10533.0
15	10262.5
16	9992.0
17	9521.0
18	9050.0
19	8925.5
20	8801.0
21	9069.0
22	9337.0
23	9696.5
24	10056.0
25	11316.5
26	14874.0
27	17171.0
28	20536.0
29	23901.0
30	26834.5
31	29768.0
32	34861.0
33	39954.0
34	45338.0
35	50722.0
36	55533.5
37	60345.0
38	65235.0
39	70125.0
40	79814.0
41	89503.0
42	99328.0
43	109153.0
44	110018.0
45	110883.0
46	109110.5
47	107338.0
48	107178.5
49	107019.0
50	102086.0
51	97153.0
52	94955.5
53	92758.0
54	83769.5
55	74781.0
56	71458.5
57	68136.0
58	60528.0
59	52920.0
60	48132.5
61	43345.0
62	38127.0
63	32909.0
64	27518.5
65	22128.0
66	18012.0
67	13896.0
68	11139.5
69	8383.0
70	7024.5
71	5666.0
72	4723.5
73	3781.0
74	3151.0
75	1834.5
76	1148.0
77	925.5
78	703.0
79	577.0
80	451.0
81	317.5
82	184.0
83	129.0
84	74.0
85	46.0
86	18.0
87	13.0
88	8.0
89	6.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1474696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.587569566486682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.66709612579349	18.083904633276752
2	5.686070607148765	2.682411720121533
3	2.5835651674780027	1.8282006935231763
4	1.6368994473235734	1.5444191834835357
5	1.1766293552578617	1.3876913385557592
6	0.8936104997412507	1.2646859896793807
7	0.782032898747167	1.2912378781736027
8	0.6556321148664844	1.2371814495548792
9	0.5736082528871729	1.2177022111998366
>10	7.879259029726668	44.31288543246071
>50	1.3789786817915848	21.195388202263246
>100	0.08488546285502382	2.7919013925604204
>500	8.661781915093722E-4	0.12919226582811394
>1k	5.774521276729148E-4	0.19620607161349302
>5k	0.0	0.0
>10k+	2.887260638364574E-4	0.8369915377055985
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12290	0.8333921025079066	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1848	0.12531396301339395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011663420799947921	0.0
2	0.0	0.0	0.0	0.03885546580447767	0.0
3	0.0	0.0	0.0	0.05709651345090785	0.0
4	0.0	0.0	0.0	0.0796774386042954	0.0
5	0.0	0.0	0.0	0.13379028626917006	0.0
6	0.0	0.0	0.0	0.2108909226037095	0.0
7	0.0	0.0	0.0	0.2547643717756066	0.0
8	0.0	0.0	0.0	0.3679402398867292	0.0
9	0.0	0.0	0.0	0.42232432989578866	0.0
10	0.0	0.0	0.0	0.5085115847605202	0.0
11	0.0	0.0	0.0	0.5868328116438913	0.0
12	0.0	0.0	0.0	0.6576948740621796	0.0
13	0.0	0.0	0.0	0.6913967353271454	0.0
14	0.0	0.0	0.0	0.7093665406293908	0.0
15	0.0	0.0	0.0	0.7313371705083624	0.0
16	0.0	0.0	0.0	0.7730406809267808	0.0
17	0.0	0.0	0.0	0.8216608711219126	0.0
18	0.0	0.0	0.0	0.8915057747495077	0.0
19	0.0	0.0	0.0	0.9246651513261038	0.0
20	0.0	0.0	0.0	0.9615541101352414	0.0
21	0.0	0.0	0.0	1.00719063454434	0.0
22	0.0	0.0	0.0	1.055539582395287	0.0
23	0.0	0.0	0.0	1.110805210022947	0.0
24	0.0	0.0	0.0	1.156848597948323	0.0
25	0.0	0.0	0.0	1.1986877295388338	0.0
26	0.0	0.0	0.0	1.2386959753060969	0.0
27	0.0	0.0	0.0	1.2839256361989182	0.0
28	0.0	0.0	0.0	1.3301046452963865	0.0
29	0.0	0.0	0.0	1.3821153647938287	0.0
30	0.0	0.0	0.0	1.4554864188958268	0.0
31	6.781058604620885E-5	0.0	0.0	1.514074765239751	0.0
32	6.781058604620885E-5	0.0	0.0	1.5822922148022371	0.0
33	6.781058604620885E-5	0.0	0.0	1.650102800848446	0.0
34	6.781058604620885E-5	0.0	0.0	1.711471381220265	0.0
35	6.781058604620885E-5	0.0	0.0	1.7824690648106458	0.0
36	6.781058604620885E-5	0.0	0.0	1.8456685310057124	0.0
37	6.781058604620885E-5	0.0	0.0	1.9126653900193666	0.0
38	6.781058604620885E-5	0.0	0.0	1.9876638981864736	0.0
39	6.781058604620885E-5	0.0	0.0	2.0940587076929753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.033937E-4	45.0	44
TAGCCGA	20	7.033937E-4	45.0	39
GGGCGTA	20	7.033937E-4	45.0	8
TAATGCG	35	1.2122473E-7	45.0	1
CGTTTTT	9775	0.0	42.05371	1
CGATCTC	55	6.184564E-11	40.909092	10
TACGGGA	370	0.0	40.743244	4
CGACGGT	75	0.0	39.0	28
TAACGGG	435	0.0	38.275864	3
ACGGGAT	505	0.0	37.87129	5
CGTTAGG	245	0.0	37.65306	2
AGGGCGA	1555	0.0	37.62058	6
CGAACGC	30	1.1400785E-4	37.500004	41
CCACTCG	30	1.1400785E-4	37.500004	29
CGTAAGG	180	0.0	37.5	2
GGGCGAT	3390	0.0	37.300884	7
GGCACCG	725	0.0	37.241383	8
CGGTCTA	85	0.0	37.058823	31
GCGATGT	445	0.0	36.91011	9
GACCGAT	865	0.0	36.6763	9
>>END_MODULE