FastQCFastQC Report
Thu 26 May 2016
SRR1545462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545462_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1369628
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162101.1835330469295313No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15460.11287736524078071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTA207.0337614E-445.018
TCGTCTA207.0337614E-445.020
CTCGATG351.2121563E-745.01
CGGACTA253.8911996E-544.9999966
CGAATAT1100.042.95454414
CGTTTTT126450.042.811391
ATACCGG501.0822987E-940.4999962
GCGTAAG1000.040.4999961
AATACGG1350.040.0000042
TCGACGG900.040.02
TATCGAG403.458863E-739.3751
TAGGGAC10700.038.691595
ACGGGAT6000.038.2500045
TCACGAC659.094947E-1238.0769225
GGCGATA5500.037.6363648
GTTTTTT150250.037.482532
TATACGG1150.037.1739122
GGGCGAT26100.036.7241367
TACGGGA5300.036.5094344
CGTAAGG2350.036.382982
Produced by FastQC (version 0.11.5)