FastQCFastQC Report
Thu 26 May 2016
SRR1545461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545461_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1773899
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99780.5624897471614787No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC95270.5370655262785536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG406.8193913E-945.00000420
AACCGTA207.034323E-445.00000410
ACTATCG207.034323E-445.0000041
CGACGGT1300.045.00000428
CCACTCG207.034323E-445.00000434
CTACGTT207.034323E-445.00000441
CGTAGAT406.8193913E-945.00000431
ACTACGT207.034323E-445.00000431
CGAATCG207.034323E-445.00000412
CCGAATC207.034323E-445.00000411
CGGTCTA1300.045.00000431
GAACCGT207.034323E-445.0000049
AGGATCG406.8193913E-945.00000418
TATACGC207.034323E-445.00000420
CGCCGAT207.034323E-445.00000421
ACGTTCA253.891666E-545.013
ATTAACG253.891666E-545.01
GGGCGTA453.8562575E-1045.07
AACGCCA502.1827873E-1145.011
CGCATCC302.1661726E-644.99999626