##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545460_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1109075
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92693550932083	31.0	31.0	33.0	28.0	34.0
2	31.214451682708564	31.0	31.0	34.0	28.0	34.0
3	31.314997633162772	31.0	31.0	34.0	28.0	34.0
4	35.1526650587201	35.0	35.0	37.0	32.0	37.0
5	35.01822149088204	35.0	35.0	37.0	32.0	37.0
6	35.071098888713564	37.0	35.0	37.0	32.0	37.0
7	35.56009196853233	37.0	35.0	37.0	35.0	37.0
8	35.47813357978496	37.0	35.0	37.0	35.0	37.0
9	37.15017199017199	39.0	37.0	39.0	34.0	39.0
10	36.524971710659784	39.0	35.0	39.0	32.0	39.0
11	36.23217906814237	38.0	35.0	39.0	31.0	39.0
12	35.52451096634583	37.0	35.0	39.0	30.0	39.0
13	35.086615422762215	37.0	34.0	39.0	29.0	39.0
14	36.102139170029076	38.0	35.0	40.0	30.0	41.0
15	36.41378716497982	38.0	35.0	40.0	31.0	41.0
16	36.48558303090413	38.0	35.0	40.0	31.0	41.0
17	36.39119356220274	38.0	35.0	40.0	31.0	41.0
18	36.34678808917341	38.0	35.0	40.0	31.0	41.0
19	36.25574014381354	37.0	35.0	40.0	30.0	41.0
20	36.11570903680995	37.0	34.0	40.0	30.0	41.0
21	35.94635890268918	37.0	34.0	40.0	30.0	41.0
22	35.84045623605257	37.0	34.0	40.0	30.0	41.0
23	35.777727385433806	37.0	34.0	40.0	30.0	41.0
24	35.66116718887361	37.0	34.0	40.0	29.0	41.0
25	35.52058427067601	36.0	34.0	40.0	29.0	41.0
26	35.360222708112616	36.0	34.0	40.0	29.0	41.0
27	35.263536731059666	36.0	34.0	40.0	29.0	41.0
28	35.312530712530716	36.0	34.0	40.0	29.0	41.0
29	35.3369844239569	36.0	34.0	40.0	29.0	41.0
30	35.2317228320898	36.0	34.0	40.0	29.0	41.0
31	35.09093433717287	36.0	34.0	40.0	27.0	41.0
32	34.88428555327638	36.0	34.0	40.0	27.0	41.0
33	34.65268534589635	36.0	33.0	40.0	25.0	41.0
34	34.48715551247661	36.0	33.0	40.0	24.0	41.0
35	34.33514685661475	36.0	33.0	40.0	24.0	41.0
36	34.15655568829881	36.0	33.0	40.0	23.0	41.0
37	34.1142573766427	36.0	33.0	40.0	23.0	41.0
38	34.065101999413926	36.0	33.0	40.0	23.0	41.0
39	33.99708766314271	36.0	33.0	40.0	23.0	41.0
40	33.83671077249059	35.0	33.0	40.0	23.0	41.0
41	33.76583278858508	35.0	33.0	40.0	23.0	41.0
42	33.72315668462458	35.0	33.0	40.0	23.0	41.0
43	33.66026012668215	35.0	33.0	40.0	23.0	41.0
44	33.582389829362306	35.0	32.0	40.0	23.0	41.0
45	33.53294592340464	35.0	32.0	40.0	23.0	41.0
46	33.47929851452787	35.0	32.0	39.0	23.0	41.0
47	33.372073123999726	35.0	32.0	39.0	23.0	41.0
48	33.31929130130965	35.0	32.0	39.0	22.0	41.0
49	33.34881590514618	35.0	32.0	39.0	22.0	41.0
50	33.256292856659826	35.0	32.0	39.0	22.0	41.0
51	33.06997903658454	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	5.0
13	5.0
14	22.0
15	49.0
16	161.0
17	418.0
18	885.0
19	1726.0
20	2824.0
21	4302.0
22	6389.0
23	9128.0
24	12585.0
25	17302.0
26	22061.0
27	26167.0
28	28763.0
29	31948.0
30	37217.0
31	44339.0
32	54276.0
33	68028.0
34	107227.0
35	138116.0
36	84410.0
37	99902.0
38	133843.0
39	176909.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.28584631336925	23.53582940738904	27.059666839483356	16.11865743975836
2	32.29727475599035	26.082906926943622	28.752789486734443	12.867028830331584
3	29.841444446949033	24.66541938101571	32.115681987241615	13.377454184793635
4	27.185267001780762	27.352072673173588	30.53481504857652	14.927845276469128
5	23.44900029303699	31.64474900254717	30.3773865608728	14.528864143543045
6	21.839009985798977	40.82032324234159	27.446205170975812	9.89446160088362
7	84.40348939431507	5.123098077226517	8.393390888803733	2.080021639654667
8	85.73991840046887	4.246421567522485	8.01947568920046	1.994184342808196
9	80.33523431688569	6.331402294705047	9.906724071861687	3.4266393165475737
10	47.85366183531321	23.686856163920382	16.355250997452835	12.104231003313572
11	41.640105493316504	22.544733223632306	21.67130266212835	14.143858620922842
12	37.93034736154002	22.034488199625816	26.40759191217907	13.627572526655097
13	22.515339359376057	34.85913937290084	26.606135743750425	16.01938552397268
14	17.34111759799833	37.544620517097584	28.924193584744046	16.190068300160043
15	16.893447242071094	23.686856163920382	44.24669206320582	15.173004530802697
16	18.968599959425646	19.5947974663571	42.972116403309066	18.464486170908188
17	18.467551788652706	20.012172305750287	31.008272659648807	30.5120032459482
18	22.757703491648446	23.21664450104817	33.75082839303023	20.274823614273156
19	30.428059418885105	23.548813200189347	26.6313820075288	19.39174537339675
20	31.865834141063498	21.994545003719317	27.147758267024326	18.991862588192863
21	24.052115501656786	27.32520343529518	28.187543673782205	20.43513738926583
22	23.654126186236276	24.6946329148164	27.09429028695084	24.556950611996484
23	22.191646191646193	28.52358947771792	26.93623064265266	22.34853368798323
24	21.280165904019118	23.430786917025447	36.58968058968059	18.699366589274845
25	19.81398913509005	24.988571557378897	34.88880373284043	20.30863557469062
26	18.774654554471066	31.74672587516624	28.338029438946872	21.14059013141582
27	19.52212429276649	31.35378581250141	30.03818497396479	19.085904920767305
28	17.605752541532357	27.99783603453328	36.42909631900458	17.967315104929785
29	19.357392421612605	25.185853075761333	35.062822622455656	20.393931880170413
30	19.904695354236637	29.703040822306875	30.52092960349841	19.871334219958072
31	27.987016207199694	26.70279286793048	26.185244460473818	19.124946464396007
32	29.829722967337645	25.781123909564275	27.03865834141064	17.35049478168744
33	27.71047945359872	27.541870477650292	25.573202894303808	19.174447174447174
34	21.011653855690554	27.21051326555914	29.053039695241527	22.72479318350878
35	22.36386177670581	25.913576629172958	30.161891666478823	21.560669927642405
36	28.1328133805198	27.762955616166625	25.148524671460454	18.95570633185312
37	22.31174627504903	33.11840948538196	27.041363298244033	17.52848094132498
38	22.06721817730992	32.39853030678719	24.950341500800217	20.583910015102674
39	22.712981538669613	31.24630886098776	26.09679237202173	19.9439172283209
40	24.613935035953386	26.54274958862115	26.190924869823952	22.652390505601513
41	18.311746275049025	26.45267452606902	27.77783287875031	27.457746320131644
42	21.35049478168744	26.635619773234453	25.93296215314564	26.080923291932468
43	22.585307576133264	27.079863850506054	26.19254784392399	24.142280729436692
44	20.611500574803326	29.639654667177602	28.756576426301194	20.992268331717874
45	19.284268421883098	34.42409214886279	24.660009467348917	21.63162996190519
46	21.37384757568244	32.83006108694182	26.034578364853594	19.761512972522148
47	22.886910263057054	27.188512949980844	27.91542501634245	22.00915177061966
48	22.649865879223675	25.45995536821225	30.799540157338324	21.09063859522575
49	21.93963438000135	26.53842165768771	31.212587065798076	20.30935689651286
50	19.87557198566373	32.170141784820686	27.319883686856162	20.634402542659423
51	19.230529946126275	32.62565651556477	26.351689470955524	21.792124067353424
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	611.0
1	1362.5
2	2114.0
3	7542.0
4	12970.0
5	9571.5
6	6173.0
7	6198.0
8	6223.0
9	6459.0
10	6695.0
11	6605.0
12	6515.0
13	6554.0
14	6593.0
15	6238.5
16	5884.0
17	5768.0
18	5652.0
19	5145.5
20	4639.0
21	5145.5
22	5652.0
23	5574.5
24	5497.0
25	6739.5
26	9203.5
27	10425.0
28	12224.5
29	14024.0
30	15919.0
31	17814.0
32	20609.0
33	23404.0
34	24255.5
35	25107.0
36	27301.0
37	29495.0
38	35294.0
39	41093.0
40	55865.5
41	70638.0
42	81803.5
43	92969.0
44	94397.5
45	95826.0
46	93901.5
47	91977.0
48	86590.0
49	81203.0
50	78084.0
51	74965.0
52	70791.5
53	66618.0
54	63307.5
55	59997.0
56	57883.0
57	55769.0
58	53944.5
59	52120.0
60	48028.0
61	43936.0
62	37989.0
63	32042.0
64	25744.0
65	19446.0
66	15395.0
67	11344.0
68	8750.0
69	6156.0
70	5152.5
71	4149.0
72	3360.5
73	2572.0
74	2010.5
75	1066.5
76	684.0
77	524.5
78	365.0
79	272.5
80	180.0
81	122.5
82	65.0
83	40.5
84	16.0
85	13.5
86	11.0
87	10.0
88	9.0
89	5.5
90	2.0
91	3.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1109075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.37348738980491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46906255813721	15.20219393959884
2	5.476195801761622	2.121860206190628
3	2.050912497918929	1.1919998224807702
4	1.3013543631930933	1.0084708937995597
5	0.8700156710350657	0.8427618815865252
6	0.7009335925271813	0.8147716869549602
7	0.6019375618969858	0.8163140835402818
8	0.5206587116947923	0.8069579988328903
9	0.43353568006870724	0.7559188227757436
>10	6.743929605754492	31.804935029694153
>50	2.418620053578471	32.85314411672089
>100	0.4091035273265208	10.377404028288264
>500	0.0014026406651195	0.15280869189569596
>1k	0.0018701875534926666	0.62165148339073
>5k	4.6754688837316664E-4	0.6288073142501015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6942	0.6259270112481121	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2885	0.26012668214503076	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1654	0.14913328674796564	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1260	0.11360818700268242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004057435250095801	0.0
2	0.0	0.0	0.0	0.017762549872641614	0.0
3	0.0	0.0	0.0	0.026959403106192097	0.0
4	0.0	0.0	0.0	0.04463178775105381	0.0
5	0.0	0.0	0.0	0.07736176543515993	0.0
6	0.0	0.0	0.0	0.12650181457520907	0.0
7	0.0	0.0	0.0	0.156526835425918	0.0
8	0.0	0.0	0.0	0.25129048982259994	0.0
9	0.0	0.0	0.0	0.2916845118679981	0.0
10	0.0	0.0	0.0	0.3550706669972725	0.0
11	0.0	0.0	0.0	0.4061041859206997	0.0
12	0.0	0.0	0.0	0.4535310957329306	0.0
13	0.0	0.0	0.0	0.4737281067556297	0.0
14	0.0	0.0	0.0	0.48256429907806053	0.0
15	0.0	0.0	0.0	0.4930234655005297	0.0
16	0.0	0.0	0.0	0.5165565899510853	0.0
17	0.0	0.0	0.0	0.5459504542073349	0.0
18	0.0	0.0	0.0	0.5901314158194892	0.0
19	0.0	0.0	0.0	0.6117710704866668	0.0
20	9.016522777990667E-5	0.0	0.0	0.6344025426594234	0.0
21	9.016522777990667E-5	0.0	0.0	0.6653292157879314	0.0
22	9.016522777990667E-5	0.0	0.0	0.6985100196109371	0.0
23	9.016522777990667E-5	0.0	0.0	0.7344859454951198	0.0
24	9.016522777990667E-5	0.0	0.0	0.7619863399679914	0.0
25	9.016522777990667E-5	0.0	0.0	0.7886752473908437	0.0
26	9.016522777990667E-5	0.0	0.0	0.8149133286747966	0.0
27	9.016522777990667E-5	0.0	0.0	0.837634966075333	0.0
28	9.016522777990667E-5	0.0	0.0	0.8638730473592859	0.0
29	9.016522777990667E-5	0.0	0.0	0.894709555260014	0.0
30	9.016522777990667E-5	0.0	0.0	0.9351937425331921	0.0
31	9.016522777990667E-5	0.0	0.0	0.9742352861618917	0.0
32	9.016522777990667E-5	0.0	0.0	1.0143588125239502	0.0
33	9.016522777990667E-5	0.0	0.0	1.0527691995581905	0.0
34	9.016522777990667E-5	0.0	0.0	1.089827108175732	0.0
35	9.016522777990667E-5	0.0	0.0	1.1425737664269775	0.0
36	9.016522777990667E-5	0.0	0.0	1.18846786736695	0.0
37	9.016522777990667E-5	0.0	0.0	1.232738994206884	0.0
38	9.016522777990667E-5	0.0	0.0	1.2789937560579763	0.0
39	9.016522777990667E-5	0.0	0.0	1.3318305795370016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCGA	30	2.1654323E-6	45.000004	27
ATGTCGC	20	7.033179E-4	45.0	25
CGTCGAA	25	3.8907165E-5	45.0	27
ACCCGTA	25	3.8907165E-5	45.0	33
CCTATAC	20	7.033179E-4	45.0	28
TGTCGTT	20	7.033179E-4	45.0	28
TCGAATC	20	7.033179E-4	45.0	30
CATACGA	290	0.0	44.224136	18
ATACGAA	290	0.0	44.224136	19
CGCATCG	65	0.0	41.538464	21
ACGGGTA	170	0.0	41.02941	5
CGGTCTA	55	6.184564E-11	40.909092	31
TACGAAA	310	0.0	40.645164	20
GTTGATC	295	0.0	40.42373	16
CGTAAGG	195	0.0	40.384617	2
CGTTTTT	5420	0.0	40.226017	1
GCGTAAG	90	0.0	40.0	1
GGCCTAT	175	0.0	39.857143	8
CGGGATA	130	0.0	39.807693	6
ACGGGCT	165	0.0	39.545452	5
>>END_MODULE