##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545459_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1269621
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88833518034122	31.0	31.0	33.0	28.0	34.0
2	31.175457872861273	31.0	31.0	34.0	28.0	34.0
3	31.26571945486094	31.0	31.0	34.0	28.0	34.0
4	35.09625313380922	35.0	35.0	37.0	32.0	37.0
5	34.960811927338945	35.0	35.0	37.0	32.0	37.0
6	35.01769189388014	37.0	35.0	37.0	32.0	37.0
7	35.52607352902953	37.0	35.0	37.0	35.0	37.0
8	35.44773046444569	37.0	35.0	37.0	35.0	37.0
9	37.143251411247924	39.0	37.0	39.0	34.0	39.0
10	36.534307482311654	39.0	35.0	39.0	32.0	39.0
11	36.199543013229935	38.0	35.0	39.0	31.0	39.0
12	35.37606813371865	37.0	35.0	39.0	30.0	39.0
13	34.84105177844412	37.0	33.0	39.0	27.0	39.0
14	35.81864036590447	38.0	34.0	40.0	28.0	41.0
15	36.208614224244876	38.0	34.0	40.0	30.0	41.0
16	36.325569599116584	38.0	35.0	40.0	31.0	41.0
17	36.252076013235445	38.0	34.0	40.0	31.0	41.0
18	36.19207464274772	37.0	35.0	40.0	30.0	41.0
19	36.091244552508186	37.0	35.0	40.0	30.0	41.0
20	35.937467953034805	37.0	34.0	40.0	30.0	41.0
21	35.75650056197873	36.0	34.0	40.0	30.0	41.0
22	35.631717654323616	36.0	34.0	40.0	30.0	41.0
23	35.571016862512515	36.0	34.0	40.0	29.0	41.0
24	35.47473852433128	36.0	34.0	40.0	29.0	41.0
25	35.31638103024446	36.0	34.0	40.0	29.0	41.0
26	35.14935559509492	35.0	34.0	40.0	29.0	41.0
27	35.026863922383136	36.0	34.0	40.0	27.0	41.0
28	35.07969071084993	36.0	34.0	40.0	28.0	41.0
29	35.135872831341004	36.0	34.0	40.0	29.0	41.0
30	35.038858052915	36.0	34.0	40.0	28.0	41.0
31	34.870189607764836	36.0	34.0	40.0	27.0	41.0
32	34.62899164396304	35.0	33.0	40.0	26.0	41.0
33	34.371163520452164	35.0	33.0	40.0	24.0	41.0
34	34.210655778377955	35.0	33.0	40.0	24.0	41.0
35	34.02569979545077	35.0	33.0	40.0	23.0	41.0
36	33.81339864416231	35.0	33.0	40.0	23.0	41.0
37	33.75064448366875	35.0	33.0	40.0	23.0	41.0
38	33.73037859329674	35.0	33.0	40.0	23.0	41.0
39	33.683444902061325	35.0	33.0	40.0	23.0	41.0
40	33.51357294814752	35.0	32.0	40.0	22.0	41.0
41	33.46203709610978	35.0	32.0	40.0	22.0	41.0
42	33.40932293968042	35.0	32.0	40.0	22.0	41.0
43	33.30960893053911	35.0	32.0	39.0	22.0	41.0
44	33.2339099621068	35.0	32.0	39.0	21.0	41.0
45	33.17819648540785	35.0	32.0	39.0	22.0	41.0
46	33.12784051303499	35.0	32.0	39.0	22.0	41.0
47	33.03984102342353	35.0	32.0	39.0	22.0	41.0
48	33.004527335322905	35.0	32.0	39.0	21.0	41.0
49	33.03835002729161	35.0	32.0	39.0	21.0	41.0
50	32.905902627634546	35.0	32.0	39.0	20.0	40.0
51	32.73003990954781	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	10.0
14	27.0
15	78.0
16	194.0
17	553.0
18	1155.0
19	2248.0
20	3570.0
21	5592.0
22	8068.0
23	11473.0
24	15334.0
25	21285.0
26	27350.0
27	31426.0
28	34068.0
29	37957.0
30	44226.0
31	52862.0
32	64247.0
33	81497.0
34	128855.0
35	168538.0
36	92414.0
37	107307.0
38	142539.0
39	186663.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.80987003208044	23.54623938955011	27.3457984705672	16.29809210780225
2	32.23284744029911	26.281543862302215	28.905949098195443	12.579659599203227
3	29.32670458349381	24.418468188538153	32.605714618772055	13.649112609195974
4	26.467819924213604	27.222218284039094	31.243182020461223	15.066779771286075
5	22.501124351282783	32.21457427058942	30.8797664814933	14.404534896634507
6	22.158108600913188	39.262740613143606	28.559152692023844	10.019998093919366
7	83.4266288916141	5.551656754259736	9.077039525968774	1.9446748281573791
8	84.79420236432762	4.627050119681385	8.616193336436622	1.962554179554371
9	80.17707646612651	6.091896715634036	10.56740554858497	3.163621269654487
10	50.57989746546411	22.292558172872063	15.996899862242353	11.13064449942148
11	44.673174120465866	21.698916448294412	20.84417318239065	12.783736248849065
12	40.4477399160852	21.086371444706728	25.248322137078706	13.217566502129378
13	21.983962143033235	38.30284785774653	24.829535743343882	14.88365425587636
14	15.515181302136622	40.927016802652126	28.35121662291345	15.206585272297795
15	15.487062674609195	22.507976789923923	47.330660094626666	14.674300440840218
16	16.297619525827	18.13887766506698	46.49836447254732	19.065138336558704
17	16.872830553369862	18.763867327336268	31.049029592295653	33.31427252699821
18	21.371574666770634	22.556180151399513	35.23161636425358	20.840628817576267
19	30.46594219849861	22.826890859555725	26.605971388311943	20.101195553633723
20	31.930710030788717	21.969627156450624	26.722620372536372	19.377042440224287
21	23.156201732643048	27.166689901947116	29.099077598747975	20.57803076666186
22	23.80513554832505	25.40112364240982	25.38119643578674	25.412544373478386
23	20.566452508268217	30.285100829302603	26.09400758179016	23.054439080639025
24	20.02605501956883	23.29923654381898	39.7409935720975	16.933714864514684
25	18.29404208027435	24.497074323754884	37.37524820399158	19.833635391979183
26	18.082325355361956	33.42430536356913	27.962202893619438	20.531166387449485
27	19.563318502135676	33.858293144174525	28.67777076781181	17.90061758587799
28	16.105042370912265	28.92532495918073	37.54663793368257	17.42299473622443
29	16.53091749427585	24.806457990219126	37.326020914902955	21.336603600602068
30	18.875317909832933	30.457199431956468	31.46568936714185	19.201793291068753
31	28.61554747440378	26.28879011925606	25.70609654377172	19.389565862568436
32	30.24059935996648	25.525964047538597	27.591777388685284	16.641659203809642
33	28.220941525069293	27.051301136323357	25.509817496717524	19.217939841889827
34	20.06409786857653	27.65053508094148	28.868378831163	23.416988219318995
35	22.03350448677204	24.31536655427092	31.169931814297335	22.481197144659706
36	29.63238635781859	26.16363465947712	25.890167223131943	18.313811759572346
37	20.455947089722052	33.707933312382195	28.24031738605458	17.59580221184117
38	20.848347656505368	33.36696541723869	24.380740394180624	21.40394653207532
39	21.802805719187067	31.707572574807756	26.685286396491552	19.804335309513625
40	24.134131366762208	26.775785844752097	26.02036355731356	23.069719231172137
41	18.335077948458633	25.662067656410848	27.265695825762176	28.737158569368336
42	21.331090144224145	26.860929363959794	24.931219631685362	26.876760860130698
43	22.096200362155322	26.676858684599576	26.558713190786857	24.668227762458244
44	20.010381050723012	30.735550215379238	28.048212813115097	21.20585592078266
45	17.90148398616595	36.497269657637986	23.74519640113073	21.85604995506533
46	20.846929910579615	33.32742605864269	25.64347943205098	20.182164598726708
47	22.386444458621906	28.256306409550568	26.854391979968828	22.502857151858706
48	23.389657228416986	24.286854108430784	30.946479303666212	21.37700935948602
49	21.409144933803077	26.2018350358099	31.41630455072813	20.97271547965889
50	19.63743510858752	33.748339071266145	26.86108689128488	19.753138928861446
51	19.405554886064426	33.07884793966073	24.52944618905957	22.98615098521527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1196.0
1	2135.5
2	3075.0
3	9199.5
4	15324.0
5	11465.0
6	7606.0
7	7698.0
8	7790.0
9	8054.0
10	8318.0
11	8405.0
12	8492.0
13	8347.5
14	8203.0
15	7813.0
16	7423.0
17	7087.5
18	6752.0
19	6287.5
20	5823.0
21	6049.0
22	6275.0
23	5864.0
24	5453.0
25	6405.5
26	9059.0
27	10760.0
28	12856.0
29	14952.0
30	17841.0
31	20730.0
32	23851.5
33	26973.0
34	28076.0
35	29179.0
36	31331.5
37	33484.0
38	38941.5
39	44399.0
40	64276.5
41	84154.0
42	99573.0
43	114992.0
44	116230.0
45	117468.0
46	112812.5
47	108157.0
48	98798.0
49	89439.0
50	86089.5
51	82740.0
52	81422.5
53	80105.0
54	74305.5
55	68506.0
56	64614.5
57	60723.0
58	57712.0
59	54701.0
60	51297.0
61	47893.0
62	39886.0
63	31879.0
64	25778.0
65	19677.0
66	15753.0
67	11829.0
68	9675.0
69	7521.0
70	5913.0
71	4305.0
72	3518.5
73	2732.0
74	2136.5
75	1231.0
76	921.0
77	692.5
78	464.0
79	311.5
80	159.0
81	126.0
82	93.0
83	56.0
84	19.0
85	17.5
86	16.0
87	15.5
88	15.0
89	8.0
90	1.0
91	2.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1269621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.150089769524925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11294844828679	15.341701544860145
2	5.737552353711376	2.1974928526184376
3	2.220431776955414	1.2756440356740577
4	1.2113481874512044	0.927897061273675
5	0.7890409187140882	0.7555102212601605
6	0.6035261824587816	0.6934548343406608
7	0.4813017628720542	0.6451880375661306
8	0.40114964412279897	0.6145641356771662
9	0.33721563443973224	0.5811938704087356
>10	5.128050313782753	23.599400808123036
>50	2.1081892354882408	29.206435202315014
>100	0.8622154873831951	22.238818090227834
>500	0.004962391294291771	0.6124698887901306
>1k	0.0016541304314305902	0.658242787124847
>5k	4.1353260785764756E-4	0.6519866297399982
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8233	0.6484612337067519	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3349	0.26377950585253396	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1902	0.14980848615452957	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1533	0.12074469467660033	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1528	0.12035087636389127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.876366254181365E-5	0.009766694155184894	0.0
2	0.0	0.0	7.876366254181365E-5	0.04072081353411766	0.0
3	0.0	0.0	7.876366254181365E-5	0.056867364355189465	0.0
4	0.0	0.0	7.876366254181365E-5	0.09081450291071115	0.0
5	0.0	0.0	7.876366254181365E-5	0.15303779631874395	0.0
6	0.0	0.0	7.876366254181365E-5	0.23046247659734675	0.0
7	0.0	0.0	7.876366254181365E-5	0.2777994377849768	0.0
8	0.0	0.0	7.876366254181365E-5	0.4146906832826489	0.0
9	0.0	0.0	7.876366254181365E-5	0.4700615380495439	0.0
10	0.0	0.0	7.876366254181365E-5	0.5374044695227946	0.0
11	0.0	0.0	7.876366254181365E-5	0.6024632547823326	0.0
12	0.0	0.0	7.876366254181365E-5	0.6702787682308342	0.0
13	0.0	0.0	7.876366254181365E-5	0.6988699777335126	0.0
14	0.0	0.0	7.876366254181365E-5	0.710133181476992	0.0
15	0.0	0.0	7.876366254181365E-5	0.7279337692114418	0.0
16	0.0	0.0	7.876366254181365E-5	0.769284692045894	0.0
17	0.0	0.0	7.876366254181365E-5	0.8104780875552625	0.0
18	0.0	0.0	7.876366254181365E-5	0.8785298919913895	0.0
19	0.0	0.0	7.876366254181365E-5	0.9031041547044354	0.0
20	7.876366254181365E-5	0.0	7.876366254181365E-5	0.9309077275816956	0.0
21	7.876366254181365E-5	0.0	7.876366254181365E-5	0.9680054126388898	0.0
22	7.876366254181365E-5	0.0	7.876366254181365E-5	1.0112466633743455	0.0
23	7.876366254181365E-5	0.0	7.876366254181365E-5	1.0619704620512735	0.0
24	7.876366254181365E-5	0.0	7.876366254181365E-5	1.0996194927462606	0.0
25	7.876366254181365E-5	0.0	7.876366254181365E-5	1.1327002310138223	0.0
26	7.876366254181365E-5	0.0	7.876366254181365E-5	1.164914568993424	0.0
27	7.876366254181365E-5	0.0	7.876366254181365E-5	1.1984678892362366	0.0
28	7.876366254181365E-5	0.0	7.876366254181365E-5	1.229658299602795	0.0
29	7.876366254181365E-5	0.0	7.876366254181365E-5	1.2688038398860761	0.0
30	7.876366254181365E-5	0.0	7.876366254181365E-5	1.3178736016496262	0.0
31	7.876366254181365E-5	0.0	7.876366254181365E-5	1.3611148523850818	0.0
32	7.876366254181365E-5	0.0	7.876366254181365E-5	1.406719012996792	0.0
33	7.876366254181365E-5	0.0	7.876366254181365E-5	1.4479124085061605	0.0
34	7.876366254181365E-5	0.0	7.876366254181365E-5	1.492492641504827	0.0
35	7.876366254181365E-5	0.0	7.876366254181365E-5	1.5452642954078422	0.0
36	7.876366254181365E-5	0.0	7.876366254181365E-5	1.5963031487349375	0.0
37	1.575273250836273E-4	0.0	7.876366254181365E-5	1.6441126918978184	0.0
38	1.575273250836273E-4	0.0	7.876366254181365E-5	1.6938913266242446	0.0
39	1.575273250836273E-4	0.0	7.876366254181365E-5	1.7502861090041832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.033566E-4	45.0	9
ATAATCG	20	7.033566E-4	45.0	44
CGACACT	20	7.033566E-4	45.0	18
TCGATAG	40	6.8157533E-9	45.0	1
ATTCACG	20	7.033566E-4	45.0	39
CGTAGCA	20	7.033566E-4	45.0	28
TCATCGG	50	2.1827873E-11	45.0	44
CTCGAGG	25	3.8910373E-5	45.0	2
TATAGCG	35	1.2120654E-7	45.0	1
TACTAGC	35	1.2120654E-7	45.0	36
TTACGAC	25	3.8910373E-5	45.0	14
ATAACGG	50	2.1827873E-11	45.0	2
ACCGTCC	20	7.033566E-4	45.0	27
ACTCGAC	30	2.1656815E-6	44.999996	34
ACGTAAG	60	0.0	44.999996	1
GGCCTAT	440	0.0	41.931816	8
ACGTATG	70	0.0	41.785713	1
TCGATGG	125	0.0	41.399998	2
GTCGATG	60	3.6379788E-12	41.249996	1
CGTTGAT	445	0.0	40.95506	25
>>END_MODULE