FastQCFastQC Report
Thu 26 May 2016
SRR1545458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545458_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1342939
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93740.6980212801921755No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53200.39614606471328934No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18130.13500240889571305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG750.045.0000041
TATAGTC302.165776E-645.00000426
ACGTAAA302.165776E-645.00000429
TTCGATC302.165776E-645.00000421
TATCGGA302.165776E-645.00000436
ATCGCAC302.165776E-645.00000422
CGGTTGA351.2121382E-745.031
CGACACG207.033712E-445.041
CCGTACC207.033712E-445.015
ATTCACG406.8157533E-945.01
TACTATC207.033712E-445.040
CTCGAAT207.033712E-445.035
GCTACGA1100.045.010
GATCTCG351.2121382E-745.010
GTCCACT207.033712E-445.045
CGGTAAT207.033712E-445.06
TCGAATA207.033712E-445.036
AATCGTC207.033712E-445.020
GTTTCGA253.891159E-544.99999624
ATCCGTG253.891159E-544.9999961