##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545458_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1342939
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.921738813155326	31.0	31.0	33.0	28.0	34.0
2	31.231070063495064	31.0	31.0	34.0	28.0	34.0
3	31.301919893606485	31.0	31.0	34.0	28.0	34.0
4	35.14178008085252	35.0	35.0	37.0	32.0	37.0
5	34.99668637220306	35.0	35.0	37.0	32.0	37.0
6	35.039222928219374	37.0	35.0	37.0	32.0	37.0
7	35.545901191342274	37.0	35.0	37.0	35.0	37.0
8	35.478296482565476	37.0	35.0	37.0	35.0	37.0
9	37.16772541418486	39.0	37.0	39.0	34.0	39.0
10	36.576352313842996	39.0	35.0	39.0	32.0	39.0
11	36.23719469015346	38.0	35.0	39.0	31.0	39.0
12	35.24542589052816	37.0	35.0	39.0	30.0	39.0
13	34.664954253320516	37.0	33.0	39.0	27.0	39.0
14	35.606369313870545	37.0	33.0	40.0	27.0	41.0
15	36.032536101788686	37.0	34.0	40.0	30.0	41.0
16	36.204164150419345	37.0	34.0	40.0	31.0	41.0
17	36.11392848074261	37.0	34.0	40.0	31.0	41.0
18	36.0309820475837	37.0	34.0	40.0	30.0	41.0
19	35.89195265012037	37.0	34.0	40.0	30.0	41.0
20	35.71371074933411	36.0	34.0	40.0	30.0	41.0
21	35.52667917157816	36.0	34.0	40.0	29.0	41.0
22	35.39260234455921	35.0	34.0	40.0	29.0	41.0
23	35.36084066364891	35.0	34.0	40.0	29.0	41.0
24	35.26018679925149	35.0	34.0	40.0	29.0	41.0
25	35.123666823288325	35.0	34.0	40.0	29.0	41.0
26	34.959553635719864	35.0	34.0	40.0	29.0	41.0
27	34.842677887826625	35.0	34.0	40.0	27.0	41.0
28	34.8923763476971	35.0	34.0	40.0	27.0	41.0
29	34.96899859189435	36.0	34.0	40.0	29.0	41.0
30	34.876331687440754	36.0	34.0	40.0	28.0	41.0
31	34.6432198335144	35.0	33.0	40.0	27.0	41.0
32	34.37895243194218	35.0	33.0	40.0	25.0	41.0
33	34.10890070211678	35.0	33.0	40.0	24.0	41.0
34	33.901647803809404	35.0	33.0	40.0	23.0	41.0
35	33.70476023110506	35.0	33.0	40.0	23.0	41.0
36	33.47575355246962	35.0	33.0	40.0	21.0	41.0
37	33.41268814145691	35.0	32.0	40.0	22.0	41.0
38	33.4146554683422	35.0	32.0	40.0	22.0	41.0
39	33.37051869072236	35.0	32.0	40.0	22.0	41.0
40	33.192583579745616	35.0	32.0	39.0	21.0	41.0
41	33.15146927745788	35.0	32.0	39.0	21.0	41.0
42	33.10437853096827	35.0	32.0	39.0	21.0	41.0
43	32.99649500088984	35.0	32.0	39.0	20.0	41.0
44	32.937090962433885	35.0	32.0	39.0	20.0	41.0
45	32.88207431610818	35.0	32.0	39.0	20.0	41.0
46	32.82070444003786	35.0	32.0	39.0	20.0	41.0
47	32.736066939749314	35.0	31.0	39.0	20.0	41.0
48	32.724962191134516	35.0	31.0	39.0	20.0	41.0
49	32.77059270748708	35.0	32.0	39.0	20.0	41.0
50	32.68300496150607	35.0	31.0	39.0	20.0	40.0
51	32.499007773249566	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	22.0
15	83.0
16	219.0
17	646.0
18	1330.0
19	2566.0
20	4238.0
21	6457.0
22	9420.0
23	12965.0
24	17760.0
25	24296.0
26	31385.0
27	35935.0
28	37943.0
29	41564.0
30	47247.0
31	56192.0
32	69034.0
33	86626.0
34	142600.0
35	188660.0
36	90895.0
37	104650.0
38	140371.0
39	189720.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.51596386730894	24.434095666296084	28.26375583701121	18.78618462938376
2	31.86704682789017	26.552062305138207	28.46071191617788	13.120178950793745
3	27.731788264396222	25.043654253841762	33.845766635714654	13.378790846047364
4	26.410656031286607	27.365874399358418	32.04635504665514	14.17711452269984
5	23.03604259016977	31.64589009627392	31.22718157712301	14.0908857364333
6	21.55585622280684	39.120094062351306	28.737641843747184	10.586407871094666
7	82.89721275500972	5.41737189850023	9.841549020469284	1.8438663260207648
8	84.56184532581152	4.2731650506836125	9.399161093690777	1.7658285298140868
9	80.06320465784373	6.060141227561341	11.104525224153889	2.77212889044104
10	52.825630948241134	20.32594183354568	16.18599206665381	10.662435151559379
11	48.03546549768828	19.564552075708576	21.159188913271564	11.24079351333158
12	43.000091590161574	20.536748132268105	24.827263189169425	11.635897088400887
13	21.21295159348265	40.03733602196377	24.760692779046554	13.989019605507025
14	14.663882722893593	41.76727312260646	28.864229871945042	14.704614282554903
15	14.108459133289003	22.21016740149776	50.793818632119546	12.887554833093684
16	15.897073508178702	16.775371033233824	49.136260098187634	18.191295360399838
17	16.244073632532828	17.36542017172783	31.613572917310467	34.77693327842888
18	22.180307519552265	22.619940295128817	35.67958038302559	19.52017180229333
19	31.148548072548344	23.307238824697173	26.52369169411269	19.02052140864179
20	32.67788038027044	21.924748629684597	26.094632742067958	19.30273824797701
21	22.52589283653241	28.58193856906382	29.066845180607608	19.82532341379616
22	22.655459406570216	25.64755361189153	26.800993939411992	24.895993042126264
23	20.05027778625835	30.312769232258503	27.160131621763906	22.476821359719242
24	20.288337742816314	22.48456556850311	40.82828780756237	16.398808881118203
25	17.62217047833148	24.403416685344606	37.74050794563267	20.23390489069124
26	16.25278586741468	34.002363472949995	29.11398060522481	20.630870054410515
27	18.21594279412542	33.04133694829028	30.91078597017437	17.831934287409926
28	15.20903034315036	28.430554180048382	40.40756877266949	15.95284670413176
29	15.813227555384124	24.38487526239092	39.45272272232767	20.349174459897284
30	17.977212665653468	30.190500089728573	32.916684972288394	18.915602272329572
31	27.898586607433394	26.674331447668138	27.12319770294853	18.303884241949934
32	30.671385669788425	26.219210254523844	27.461783446604798	15.647620629082928
33	28.50136901229319	27.121485041390564	25.994628199791652	18.382517746524602
34	19.05954030674513	27.666260343917333	30.36251088098566	22.911688468351876
35	20.79513663688373	25.946673676168462	32.07137479811071	21.186814888837098
36	29.315553424243397	26.528457361056606	26.692575016437825	17.46341419826217
37	20.09785999215154	33.507553209788384	29.449960124771117	16.94462667328896
38	20.66296384273597	33.75521896378019	24.37593963687107	21.205877556612773
39	21.503359422877732	32.39834422859117	27.061169569131582	19.03712677939951
40	23.967060305792	26.617664689163096	26.47625841531149	22.939016589733413
41	16.673207048123555	25.832744450790397	28.12108368287763	29.372964818208423
42	21.218089578156565	27.712278815344554	24.317411289716063	26.752220316782815
43	22.18976438989411	27.564245285899062	26.122482108271484	24.123508215935345
44	19.6341010276714	31.350195355112927	28.0813946128603	20.934309004355374
45	17.54956852098271	37.678926593091724	23.31647230440102	21.455032581524552
46	21.04607878689948	34.111973812660146	25.70079504728063	19.14115235315975
47	22.162659659150567	27.4084675476697	27.611157319878266	22.817715473301465
48	23.413349377745377	23.838610688944172	32.11605292570995	20.631987007600493
49	20.469582013777245	24.96263791579513	32.79694759032242	21.770832480105202
50	18.60032361857091	33.72312517545473	26.953495281617407	20.723055924356952
51	18.10097107910337	34.00340596259398	25.16689142246967	22.728731535832974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	888.0
1	1884.0
2	2880.0
3	10216.5
4	17553.0
5	13148.5
6	8744.0
7	8954.5
8	9165.0
9	9713.5
10	10262.0
11	10232.5
12	10203.0
13	10126.0
14	10049.0
15	9837.5
16	9626.0
17	8775.0
18	7924.0
19	7485.0
20	7046.0
21	6744.5
22	6443.0
23	6604.0
24	6765.0
25	8021.5
26	9392.5
27	9507.0
28	13296.0
29	17085.0
30	19841.0
31	22597.0
32	24858.5
33	27120.0
34	29161.0
35	31202.0
36	33174.5
37	35147.0
38	46221.5
39	57296.0
40	74082.0
41	90868.0
42	109624.0
43	128380.0
44	129972.0
45	131564.0
46	125723.5
47	119883.0
48	107931.0
49	95979.0
50	91244.5
51	86510.0
52	80116.5
53	73723.0
54	70548.0
55	67373.0
56	63167.5
57	58962.0
58	56369.5
59	53777.0
60	49735.5
61	45694.0
62	37717.0
63	29740.0
64	24232.0
65	18724.0
66	14539.0
67	10354.0
68	8389.5
69	6425.0
70	5026.0
71	3627.0
72	2811.0
73	1995.0
74	1480.5
75	886.5
76	807.0
77	613.5
78	420.0
79	280.0
80	140.0
81	137.0
82	134.0
83	99.5
84	65.0
85	47.0
86	29.0
87	22.0
88	15.0
89	10.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1342939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.533421512437947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.28665572049911	15.250532753168736
2	5.683428188862903	2.1066674051973595
3	1.9778534707733961	1.0996917619104518
4	1.0391414533272676	0.7703538626224646
5	0.6616440259031742	0.613126381162497
6	0.45501689118872213	0.505981190380782
7	0.3789901690377344	0.4916789186292509
8	0.31334317614602175	0.464585692924825
9	0.26112447873583033	0.43555770284641054
>10	3.9038066656567345	17.777723594355198
>50	1.696624690857268	23.2359251426617
>100	1.3367223780090827	35.43270887044293
>500	0.0036313013589138977	0.3996898277519853
>1k	0.0012104337863046326	0.31698506638740237
>5k	8.069558575364217E-4	1.0987918295580303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9374	0.6980212801921755	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5320	0.39614606471328934	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1813	0.13500240889571305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.44635459987386E-5	0.0	0.0	0.0038721043919344067	0.0
2	1.489270919974772E-4	0.0	0.0	0.020849792879646806	0.0
3	1.489270919974772E-4	0.0	0.0	0.033731986337428584	0.0
4	1.489270919974772E-4	0.0	0.0	0.05748585751102619	0.0
5	1.489270919974772E-4	0.0	0.0	0.09568565660837909	0.0
6	1.489270919974772E-4	0.0	0.0	0.15287365993541033	0.0
7	1.489270919974772E-4	0.0	0.0	0.18533976599086036	0.0
8	1.489270919974772E-4	0.0	0.0	0.28623787081915114	0.0
9	1.489270919974772E-4	0.0	0.0	0.33314990479835643	0.0
10	1.489270919974772E-4	0.0	0.0	0.4038902734971581	0.0
11	1.489270919974772E-4	0.0	0.0	0.4601847142722045	0.0
12	1.489270919974772E-4	0.0	0.0	0.5147664934892798	0.0
13	1.489270919974772E-4	0.0	0.0	0.5364353853749128	0.0
14	1.489270919974772E-4	0.0	0.0	0.5459667192627513	0.0
15	1.489270919974772E-4	0.0	0.0	0.557434105346557	0.0
16	1.489270919974772E-4	0.0	0.0	0.5843154454521017	0.0
17	1.489270919974772E-4	0.0	0.0	0.614994426403582	0.0
18	1.489270919974772E-4	0.0	0.0	0.6626510958427747	0.0
19	1.489270919974772E-4	0.0	0.0	0.6840965970904114	0.0
20	1.489270919974772E-4	0.0	0.0	0.7105311559199636	0.0
21	1.489270919974772E-4	0.0	0.0	0.7394974753134729	0.0
22	1.489270919974772E-4	0.0	0.0	0.768240404068986	0.0
23	1.489270919974772E-4	0.0	0.0	0.8027170258664019	0.0
24	1.489270919974772E-4	0.0	0.0	0.8295239024259479	0.0
25	1.489270919974772E-4	0.0	0.0	0.8529799194155505	0.0
26	1.489270919974772E-4	0.0	0.0	0.8764359364051532	0.0
27	2.2339063799621577E-4	0.0	0.0	0.9001898075787508	0.0
28	2.2339063799621577E-4	0.0	0.0	0.9232735068383597	0.0
29	2.2339063799621577E-4	0.0	0.0	0.9513462636798842	0.0
30	2.2339063799621577E-4	0.0	0.0	0.9894715992312384	0.0
31	2.2339063799621577E-4	0.0	0.0	1.0246928564886417	0.0
32	2.2339063799621577E-4	0.0	0.0	1.0570100354520942	0.0
33	2.2339063799621577E-4	0.0	0.0	1.0926036104394914	0.0
34	2.978541839949544E-4	0.0	0.0	1.132292680456819	0.0
35	2.978541839949544E-4	0.0	0.0	1.1797259592580154	0.0
36	2.978541839949544E-4	0.0	0.0	1.2200107376433331	0.0
37	2.978541839949544E-4	0.0	0.0	1.2583594638326834	0.0
38	2.978541839949544E-4	0.0	0.0	1.2978996067580135	0.0
39	2.978541839949544E-4	0.0	0.0	1.3434712969092415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	75	0.0	45.000004	1
TATAGTC	30	2.165776E-6	45.000004	26
ACGTAAA	30	2.165776E-6	45.000004	29
TTCGATC	30	2.165776E-6	45.000004	21
TATCGGA	30	2.165776E-6	45.000004	36
ATCGCAC	30	2.165776E-6	45.000004	22
CGGTTGA	35	1.2121382E-7	45.0	31
CGACACG	20	7.033712E-4	45.0	41
CCGTACC	20	7.033712E-4	45.0	15
ATTCACG	40	6.8157533E-9	45.0	1
TACTATC	20	7.033712E-4	45.0	40
CTCGAAT	20	7.033712E-4	45.0	35
GCTACGA	110	0.0	45.0	10
GATCTCG	35	1.2121382E-7	45.0	10
GTCCACT	20	7.033712E-4	45.0	45
CGGTAAT	20	7.033712E-4	45.0	6
TCGAATA	20	7.033712E-4	45.0	36
AATCGTC	20	7.033712E-4	45.0	20
GTTTCGA	25	3.891159E-5	44.999996	24
ATCCGTG	25	3.891159E-5	44.999996	1
>>END_MODULE