FastQCFastQC Report
Thu 26 May 2016
SRR1545457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545457_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1047728
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66670.6363292763007192No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50170.47884565459737644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT351.2117926E-745.00000425
CGGGTGT351.2117926E-745.0000046
CTATGCG453.8562575E-1045.0000041
TACTTGA302.1653177E-645.00000415
ACCATCG453.8562575E-1045.00000437
GTAGGCC351.2117926E-745.00000423
ACCCTAT302.1653177E-645.00000418
GACTTAT302.1653177E-645.00000424
CGTTACG302.1653177E-645.00000424
GGCGGTA302.1653177E-645.00000437
TCGTATC302.1653177E-645.00000429
CCGACAG302.1653177E-645.00000444
TATAGCG302.1653177E-645.0000041
GTCTTAC302.1653177E-645.00000431
CGCCCAC453.8562575E-1045.00000416
TCGCATA253.8905688E-545.01
ACCGGGT253.8905688E-545.04
GATCGAT207.033002E-445.021
GTACAAC406.8139343E-945.036
TAGCGTG253.8905688E-545.01
Produced by FastQC (version 0.11.5)