##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545457_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1047728
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.963379808499916	31.0	31.0	33.0	30.0	34.0
2	31.25016893697601	31.0	31.0	34.0	30.0	34.0
3	31.33699108929035	31.0	31.0	34.0	28.0	34.0
4	35.15762106195501	35.0	35.0	37.0	32.0	37.0
5	35.023980460577555	35.0	35.0	37.0	32.0	37.0
6	35.07611612937709	37.0	35.0	37.0	32.0	37.0
7	35.582587274559806	37.0	35.0	37.0	35.0	37.0
8	35.52318254356092	37.0	35.0	37.0	35.0	37.0
9	37.267224890429574	39.0	37.0	39.0	34.0	39.0
10	36.68828646366232	39.0	37.0	39.0	32.0	39.0
11	36.320712055037184	38.0	35.0	39.0	32.0	39.0
12	35.03461299115801	35.0	34.0	39.0	30.0	39.0
13	34.33022597468045	35.0	33.0	39.0	25.0	39.0
14	35.21382648931784	36.0	33.0	40.0	26.0	41.0
15	35.73278083624758	36.0	33.0	40.0	30.0	41.0
16	35.95446337217293	36.0	34.0	40.0	31.0	41.0
17	35.90673915367347	36.0	34.0	40.0	31.0	41.0
18	35.86775957118641	36.0	34.0	40.0	30.0	41.0
19	35.746907594337465	36.0	34.0	40.0	30.0	41.0
20	35.56287223401493	35.0	34.0	40.0	30.0	41.0
21	35.34230640013438	35.0	34.0	40.0	30.0	41.0
22	35.23067151016294	35.0	34.0	40.0	29.0	41.0
23	35.24331124108547	35.0	34.0	40.0	29.0	41.0
24	35.101138845196466	35.0	34.0	40.0	29.0	41.0
25	35.0050280225402	35.0	34.0	39.0	29.0	41.0
26	34.849705267015864	35.0	34.0	39.0	29.0	41.0
27	34.69294893331094	35.0	34.0	39.0	28.0	41.0
28	34.7861324694959	35.0	34.0	39.0	29.0	41.0
29	34.89542037628087	35.0	34.0	39.0	29.0	41.0
30	34.8370703083243	35.0	34.0	39.0	29.0	41.0
31	34.58727551425561	35.0	33.0	39.0	27.0	41.0
32	34.29237931982347	35.0	33.0	39.0	26.0	41.0
33	33.98968148221676	35.0	33.0	39.0	24.0	41.0
34	33.82136489623261	35.0	33.0	39.0	23.0	41.0
35	33.626361994716184	35.0	33.0	39.0	23.0	41.0
36	33.36305701479773	35.0	33.0	39.0	22.0	41.0
37	33.28786097154987	35.0	32.0	39.0	23.0	41.0
38	33.34990188293145	35.0	32.0	39.0	23.0	41.0
39	33.351409907915034	35.0	33.0	39.0	23.0	41.0
40	33.121276705404455	35.0	32.0	39.0	22.0	41.0
41	33.179144778033994	35.0	32.0	39.0	22.0	41.0
42	33.13982445825634	35.0	32.0	39.0	22.0	41.0
43	33.02742982911595	35.0	32.0	39.0	22.0	41.0
44	32.95724462837683	35.0	32.0	39.0	22.0	41.0
45	32.92579371745339	35.0	32.0	38.0	22.0	41.0
46	32.85466266053785	35.0	32.0	38.0	22.0	40.0
47	32.7979866912023	35.0	32.0	38.0	22.0	40.0
48	32.819504680604126	35.0	32.0	38.0	22.0	40.0
49	32.87197822335568	35.0	32.0	38.0	21.0	40.0
50	32.71979273246492	35.0	32.0	38.0	20.0	40.0
51	32.50215800284043	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	7.0
14	8.0
15	32.0
16	120.0
17	389.0
18	812.0
19	1681.0
20	2858.0
21	4475.0
22	6846.0
23	9646.0
24	13548.0
25	18441.0
26	24074.0
27	27475.0
28	28756.0
29	31712.0
30	36855.0
31	44361.0
32	55259.0
33	71021.0
34	123949.0
35	171927.0
36	64943.0
37	73096.0
38	98889.0
39	136492.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.44613869248507	23.879289281187482	28.012709405494558	16.66186262083289
2	31.249140998427073	25.912832338164105	30.747102301360655	12.090924362048165
3	27.388310706595604	24.994655101324007	33.861651115556704	13.755383076523678
4	24.75537544095414	27.912015332223632	32.05097124444512	15.281637982377106
5	21.776548875280607	32.33472809736878	31.958008185330545	13.930714842020068
6	20.02246766336301	39.506055006642946	30.240386817952754	10.231090512041293
7	84.60048791289341	5.17777514774827	8.534562405509828	1.6871745338484796
8	86.94976177023044	3.844700151184277	7.732636714872562	1.4729013637127193
9	83.52196371577357	4.470816853229082	9.564218957592047	2.443000473405311
10	58.42031519631049	18.240134844158025	13.946558648809615	9.392991310721866
11	54.090660934899134	18.067189194141992	17.99999618221523	9.842153688743643
12	47.96378449368539	20.42037628086679	21.77158575508147	9.844253470366354
13	20.255066200387887	48.08471282622971	20.491196188323688	11.169024785058717
14	13.361769466884535	48.44978849472382	26.272372218743794	11.916069819647849
15	11.49105493028725	21.17457966189698	56.21869416489776	11.11567124291801
16	12.624459783455247	15.243841913168305	54.077298688209154	18.054399615167295
17	13.259452835086968	15.548978360795932	29.945749278438676	41.24581952567842
18	19.552880136829405	20.61260174396408	40.07385504634791	19.760663072858605
19	31.54664187651757	21.325095826397693	26.586480460577555	20.541781836507187
20	34.2434295924133	20.327985889467495	25.515687277614035	19.91289724050517
21	20.394701678298187	29.426244216056073	29.37527678939572	20.80377731625002
22	22.05553349724356	24.45357955499901	24.501683643082938	28.989203304674493
23	17.69505062382603	32.18039414809951	24.823904677549898	25.300650550524562
24	17.78868179527511	21.33339950826932	45.66996396011179	15.207954736343785
25	14.717178504344638	23.315975138585586	41.92004031580716	20.04680604126262
26	14.576970358719057	36.73577493395233	29.138478689125424	19.5487760182032
27	16.07211031870867	38.25277171174198	28.8009865155842	16.874131453965152
28	12.516034696027978	29.44609669685262	42.993792281966314	15.044076325153094
29	12.855435761953485	22.72526839026923	42.95943221904922	21.45986362872807
30	15.971129911580103	32.4029710001069	34.818006200082465	16.807892888230533
31	30.684299741917748	26.10467602278454	26.314463295817237	16.896560939480477
32	33.2793434937312	24.988355756455874	27.289620970328176	14.442679779484752
33	31.605435761953483	26.079001420215935	24.853969732602355	17.461593085228227
34	18.488672632591665	26.5625238611548	28.87037475375288	26.07842875250065
35	19.398259853702488	24.65449047844479	33.86861857275934	22.07863109509338
36	33.4763411877892	23.560122474535376	25.693309713971562	17.270226623703863
37	19.020585495472105	35.24454820335049	29.647866621871326	16.08699967930608
38	20.3158644228273	34.66653558938961	24.214968006963637	20.80263198081945
39	18.695882900905577	34.63704320205244	27.641048058274663	19.026025838767314
40	25.19518424629293	24.513805109723133	25.951487408945834	24.3395232350381
41	15.274193302078402	24.224035245788983	27.74422369164516	32.757547760487455
42	20.973573293831986	24.231193592230042	24.589588137379167	30.205644976558805
43	21.775785318326893	26.01009040514332	25.74150924667471	26.472615029855078
44	18.12149718247484	30.620924514759558	30.267206755951925	20.990371546813677
45	14.787043965609396	41.96604462226837	21.660201884458562	21.58670952766367
46	20.636749232625263	35.55321610188904	25.876467938243515	17.933566727242184
47	21.587950307713452	28.172388253439824	25.552051677534628	24.687609761312096
48	22.726031947222943	23.37725058412107	32.791621489546905	21.10509597910908
49	20.724558282302276	23.15352839668311	35.07475222576852	21.047161095246096
50	18.82177435364904	36.944989539269734	25.519600507001815	18.71363560007941
51	17.056717010521815	36.77500267244934	22.872443993097445	23.2958363239314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	820.0
1	1171.5
2	1523.0
3	5367.5
4	9212.0
5	7189.0
6	5166.0
7	5711.5
8	6257.0
9	6888.0
10	7519.0
11	7824.0
12	8129.0
13	7690.0
14	7251.0
15	6856.5
16	6462.0
17	6034.5
18	5607.0
19	5203.0
20	4799.0
21	4767.5
22	4736.0
23	4131.5
24	3527.0
25	4070.5
26	4911.0
27	5208.0
28	7249.5
29	9291.0
30	11417.0
31	13543.0
32	15482.0
33	17421.0
34	20953.5
35	24486.0
36	27226.0
37	29966.0
38	36527.0
39	43088.0
40	64162.5
41	85237.0
42	105339.5
43	125442.0
44	122363.0
45	119284.0
46	110656.5
47	102029.0
48	92864.0
49	83699.0
50	76819.5
51	69940.0
52	66849.5
53	63759.0
54	57129.5
55	50500.0
56	43380.5
57	36261.0
58	32820.5
59	29380.0
60	26645.0
61	23910.0
62	20259.0
63	16608.0
64	13390.5
65	10173.0
66	7943.5
67	5714.0
68	4332.0
69	2950.0
70	2380.0
71	1810.0
72	1476.0
73	1142.0
74	885.5
75	452.0
76	275.0
77	231.0
78	187.0
79	137.5
80	88.0
81	61.5
82	35.0
83	29.5
84	24.0
85	18.0
86	12.0
87	13.0
88	14.0
89	7.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1047728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.947587143915225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.37765187963636	14.964276729648008
2	5.726837876920727	2.055658437102184
3	1.7944248366493356	0.9661678838690938
4	0.9236752385623576	0.6631096734709839
5	0.537777521627254	0.4825904466721948
6	0.3955265962400742	0.4259248832252942
7	0.3047988720925929	0.3829283022774217
8	0.25150561393280907	0.36111351385943924
9	0.21970219944122538	0.35488119331631124
>10	3.3193728287373205	14.930229236996285
>50	1.5887434467802684	20.95505205653108
>100	1.5557222956906336	41.941935152674326
>500	0.0031955952667387204	0.39927403141181855
>1k	0.0	0.0
>5k	0.00106519842224624	1.11685845894558
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6667	0.6363292763007192	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5017	0.47884565459737644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018707145365972847	0.0
2	0.0	0.0	0.0	0.0625162255852664	0.0
3	0.0	0.0	0.0	0.08360948643159294	0.0
4	0.0	0.0	0.0	0.12923201441595528	0.0
5	0.0	0.0	0.0	0.20778293602919842	0.0
6	0.0	0.0	0.0	0.302845776766489	0.0
7	0.0	0.0	0.0	0.35371775880762946	0.0
8	0.0	0.0	0.0	0.49698013224806437	0.0
9	0.0	0.0	0.0	0.5527197898691263	0.0
10	0.0	0.0	0.0	0.6527457508055526	0.0
11	0.0	0.0	0.0	0.7407456897209963	0.0
12	0.0	0.0	0.0	0.8213009483377365	0.0
13	0.0	0.0	0.0	0.8526067834399768	0.0
14	0.0	0.0	0.0	0.8655872516531008	0.0
15	0.0	0.0	0.0	0.8849625093535727	0.0
16	0.0	0.0	0.0	0.9323030404837896	0.0
17	0.0	0.0	0.0	0.9819342424751463	0.0
18	0.0	0.0	0.0	1.0670708428141655	0.0
19	0.0	0.0	0.0	1.0995220133469756	0.0
20	9.544461921414718E-5	0.0	0.0	1.132927630071927	0.0
21	9.544461921414718E-5	0.0	0.0	1.1768321549104348	0.0
22	9.544461921414718E-5	0.0	0.0	1.2226455721332254	0.0
23	9.544461921414718E-5	0.0	0.0	1.2782897851350732	0.0
24	9.544461921414718E-5	0.0	0.0	1.3130316265290227	0.0
25	9.544461921414718E-5	0.0	0.0	1.3491096925919703	0.0
26	9.544461921414718E-5	0.0	0.0	1.3814654185055664	0.0
27	9.544461921414718E-5	0.0	0.0	1.4148710352305178	0.0
28	9.544461921414718E-5	0.0	0.0	1.4488493196707541	0.0
29	9.544461921414718E-5	0.0	0.0	1.481777713299635	0.0
30	9.544461921414718E-5	0.0	0.0	1.5399034864010506	0.0
31	9.544461921414718E-5	0.0	0.0	1.5911572469190476	0.0
32	9.544461921414718E-5	0.0	0.0	1.6358253287112685	0.0
33	9.544461921414718E-5	0.0	0.0	1.675625734923568	0.0
34	9.544461921414718E-5	0.0	0.0	1.7208664844310737	0.0
35	9.544461921414718E-5	0.0	0.0	1.7931180611761832	0.0
36	9.544461921414718E-5	0.0	0.0	1.84551715712475	0.0
37	9.544461921414718E-5	0.0	0.0	1.8969618068811753	0.0
38	9.544461921414718E-5	0.0	0.0	1.9398164409083274	0.0
39	9.544461921414718E-5	0.0	0.0	1.9937426507643206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	35	1.2117926E-7	45.000004	25
CGGGTGT	35	1.2117926E-7	45.000004	6
CTATGCG	45	3.8562575E-10	45.000004	1
TACTTGA	30	2.1653177E-6	45.000004	15
ACCATCG	45	3.8562575E-10	45.000004	37
GTAGGCC	35	1.2117926E-7	45.000004	23
ACCCTAT	30	2.1653177E-6	45.000004	18
GACTTAT	30	2.1653177E-6	45.000004	24
CGTTACG	30	2.1653177E-6	45.000004	24
GGCGGTA	30	2.1653177E-6	45.000004	37
TCGTATC	30	2.1653177E-6	45.000004	29
CCGACAG	30	2.1653177E-6	45.000004	44
TATAGCG	30	2.1653177E-6	45.000004	1
GTCTTAC	30	2.1653177E-6	45.000004	31
CGCCCAC	45	3.8562575E-10	45.000004	16
TCGCATA	25	3.8905688E-5	45.0	1
ACCGGGT	25	3.8905688E-5	45.0	4
GATCGAT	20	7.033002E-4	45.0	21
GTACAAC	40	6.8139343E-9	45.0	36
TAGCGTG	25	3.8905688E-5	45.0	1
>>END_MODULE