FastQCFastQC Report
Thu 26 May 2016
SRR1545456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545456_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1562740
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113870.7286560784263537No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29470.1885790342603376No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16700.10686358575322831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG302.1660053E-645.00000440
CTATACG207.034067E-445.01
TATAGCG900.045.01
ACCGTTG253.8914517E-545.01
TTCGATA406.8175723E-945.015
CCGTTAT207.034067E-445.029
CGTAAGG1900.041.4473722
ACGGGAT4350.041.379315
GTATGCG603.6379788E-1241.2500041
CACGACG1750.039.85714326
ATATACG403.4592813E-739.3751
CGTTTTT86600.039.206121
ATACTAT12900.039.06976745
ACGTTAG750.039.01
AGTTACG1100.038.8636321
ATTATAC13100.038.6450442
CGAGTCA356.249731E-638.5714317
CGTTGAT700.038.5714325
CATCGTT356.249731E-638.5714336
GTTGATC8650.038.4971116
Produced by FastQC (version 0.11.5)