FastQCFastQC Report
Thu 26 May 2016
SRR1545455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545455_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1665491
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142090.8531418062301147No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22240.13353419502116795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC750.042.012
CGTTTTT109450.041.7519381
TCGACGG700.038.571432
GCCCTAC11850.038.1645626
GGGCGAT38350.037.8422477
CGAGGGC4000.037.68754
ACGCATT301.1401421E-437.49999613
TATACTA11750.037.14893744
AGGGCGA19450.036.9023136
GGCGATT10750.036.6279078
AAGGGCG8700.036.2068985
TTTACGG1750.036.02
TAACGCG504.881258E-836.01
TCGGTAA250.0021073836.015
TCGTCCC4700.035.90425538
TATGGGC6400.035.8593754
TGGGCGA11350.035.682826
ACCCACC3850.035.64935313
GCGTAAG1200.035.6251
TACGGGA4050.035.5555534
Produced by FastQC (version 0.11.5)